NFAT2 (NFATC1) (NM_001278673) Human Tagged ORF Clone

CAT#: RG238423

NFATC1 (GFP-tagged) - Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1), transcript variant 10

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NFATC1
• Synonyms: NF-ATC; NF-ATc1.2; NFAT2; NFATc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238423 representing NM_001278673.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCAGCTTTTCATTGGGACGGCGGACGACCGCCTGCTGCGCCCGCACGCCTTCTACCAGGTGCAC
  CGCATCACAGGGAAGACCGTGTCCACCACCAGCCACGAGGCCATCCTCTCCAACACCAAAGTCCTGGAG
  ATCCCACTCCTGCCGGAGAACAGCATGCGAGCCGTCATTGACTGTGCCGGAATCCTGAAACTCAGAAAC
  TCCGACATTGAACTTCGGAAAGGAGAGACGGACATCGGGAGGAAGAACACACGGGTACGGCTGGTGTTC
  CGCGTTCACGTCCCGCAACCCAGCGGCCGCACGCTGTCCCTGCAGGTGGCCTCCAACCCCATCGAATGC
  TCCCAGCGCTCAGCTCAGGAGCTGCCTCTGGTGGAGAAGCAGAGCACGGACAGCTATCCGGTCGTGGGC
  GGGAAGAAGATGGTCCTGTCTGGCCACAACTTCCTGCAGGACTCCAAGGTCATTTTCGTGGAGAAAGCC
  CCAGATGGCCACCATGTCTGGGAGATGGAAGCGAAAACTGACCGGGACCTGTGCAAGCCGAATTCTCTG
  GTGGTTGAGATCCCGCCATTTCGGAATCAGAGGATAACCAGCCCCGTTCACGTCAGTTTCTACGTCTGC
  AACGGGAAGAGAAAGCGAAGCCAGTACCAGCGTTTCACCTACCTTCCCGCCAACGTTCCAATTATAAAA
  ACAGAACCCACTGATGATTATGAGCCTGCTCCAACCTGTGGACCGGTGAGCCAGGGGTTAAGTCCTCTC
  CCAAGACCATACTACAGCCAGCAGCTCGCGATGCCACCCGACCCCAGCTCCTGCCTCGTGGCCGGCTTC
  CCGCCCTGTCCGCAGAGAAGCACCCTGATGCCAGCGGCCCCTGGCGTGAGCCCCAAGCTCCACGACCTT
  TCTCCCGCTGCCTACACCAAGGGCGTTGCCAGCCCGGGCCACTGTCACCTCGGACTCCCGCAGCCGGCC
  GGAGAGGCCCCCGCCGTCCAGGACGTGCCCAGGCCAGTGGCCACGCACCCCGGCTCGCCCGGGCAGCCA
  CCCCCGGCCCTGCTGCCACAGCAGGTGAGTGCGCCTCCAAGCAGTAGCTGCCCCCCTGGTCTCGAACAC
  TCGCTCTGCCCCAGCAGCCCCTCTCCTCCACTCCCGCCTGCCACCCAAGAGCCGACCTGCCTGCAGCCC
  TGCAGCCCAGCGTGCCCGCCCGCCACGGGCCGCCCGCAGCACCTGCCGTCCACGGTCCGCAGGGACGAG
  TCTCCGACTGCCGGGCCACGGCTGCTGCCAGAGGTGCATGAGGACGGTAGTCCTAATTTGGCCCCTATT
  CCTGTAACGGTCAAGCGAGAGCCTGAAGAGTTGGACCAGTTGTACCTGGATGACGTAAATGAAATAATA
  CGAAATGACCTCTCCAGCACGAGCACCCACTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238423
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLQLFIGTADDRLLRPHAFYQVHRITGKTVSTTSHEAILSNTKVLEIPLLPENSMRAVIDCAGILKLRN
  SDIELRKGETDIGRKNTRVRLVFRVHVPQPSGRTLSLQVASNPIECSQRSAQELPLVEKQSTDSYPVVG
  GKKMVLSGHNFLQDSKVIFVEKAPDGHHVWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPVHVSFYVC
  NGKRKRSQYQRFTYLPANVPIIKTEPTDDYEPAPTCGPVSQGLSPLPRPYYSQQLAMPPDPSSCLVAGF
  PPCPQRSTLMPAAPGVSPKLHDLSPAAYTKGVASPGHCHLGLPQPAGEAPAVQDVPRPVATHPGSPGQP
  PPALLPQQVSAPPSSSCPPGLEHSLCPSSPSPPLPPATQEPTCLQPCSPACPPATGRPQHLPSTVRRDE
  SPTAGPRLLPEVHEDGSPNLAPIPVTVKREPEELDQLYLDDVNEIIRNDLSSTSTHS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278673
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278673.1, NP_001265602.1
• RefSeq Size: 3558 bp
• RefSeq ORF: 1416 bp
• Locus ID: 4772
• Cytogenetics: 18q23
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 51.6 kDa
• Gene Summary: 'The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]'