LAMA3 (NM_001302996) Human Tagged ORF Clone

CAT#: RG238518

LAMA3 (GFP-tagged) - Human laminin, alpha 3 (LAMA3), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: LAMA3
• Synonyms: BM600; E170; LAMNA; LOCS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238518 representing NM_001302996.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCGGCCGCGCGGCCTCGGGGTCGGGCACTGGGGCCAGTACTGCCGCCGACGCCGCTGCTCCTG
  CTGGTACTGCGGGTGCTGCCAGCCTGCGGGGCGACCGCTCGGGATCCCGGGGCCGCGGCCGGGCTCAGC
  CTTCACCCGACTTACTTCAACCTGGCCGAGGCGGCGAGGATTTGGGCCACCGCCACCTGCGGGGAGAGG
  GGACCCGGCGAGGGGAGGCCCCAGCCCGAGCTCTACTGCAAGTTGGTCGGGGGCCCCACCGCCCCAGGC
  AGCGGCCACACCATCCAGGGCCAGTTCTGTGACTATTGCAATTCTGAAGACCCCAGGAAAGCACATCCT
  GTCACCAATGCCATCGATGGATCTGAACGTTGGTGGCAAAGCCCTCCCCTGTCCTCAGGCACACAGTAC
  AACAGAGTCAACCTCACCTTGGATCTGGGGCAGCTCTTCCATGTGGCCTATATTTTAATCAAATTTGCA
  AATTCTCCTCGCCCTGATCTTTGGGTCTTGGAAAGATCTGTAGACTTTGGAAGCACCTACTCACCATGG
  CAATATTTTGCTCATTCTAAAGTAGACTGTTTAAAAGAATTTGGGCGGGAGGCAAATATGGCTGTCACC
  CGGGATGATGATGTACTTTGTGTTACTGAATATTCCCGTATTGTACCTTTGGAAAATGGTGAGGTTGTG
  GTGTCCTTGATAAACGGTCGTCCAGGTGCAAAAAATTTTACTTTCTCTCACACCCTGAGGGAGTTTACC
  AAGGCAACAAACATCCGCTTGCGTTTTCTTAGAACCAATACGCTTCTTGGACACCTCATCTCCAAAGCC
  CAGCGAGATCCAACTGTCACTCGGCGGTATTATTACAGCATAAAGGACATCAGCATTGGTGGGCAGTGT
  GTTTGCAATGGCCATGCTGAAGTGTGCAATATAAACAATCCTGAAAAACTGTTTCGGTGTGAATGCCAG
  CACCACACCTGTGGGGAGACGTGTGATCGCTGCTGCACAGGGTACAATCAGAGGCGCTGGCGGCCCGCC
  GCTTGGGAGCAGAGCCACGAGTGTGAAGCATGCAACTGCCACGGCCATGCCAGCAACTGTTACTATGAT
  CCAGATGTTGAGCGGCAGCAGGCAAGCTTGAATACCCAGGGCATCTATGCTGGTGGAGGGGTCTGCATT
  AACTGTCAGCACAACACAGCTGGAGTAAACTGTGAACAGTGTGCTAAGGGCTATTACCGCCCTTATGGG
  GTTCCAGTGGATGCCCCTGATGGCTGCATCCGTAAGTTTCATTTCAAGTTGGTGTATCTTAGCCTGTGT
  GTACTGCCTCAGCGAAGTCATCAGGCTAACTTTGGATCAGTTAATAACTTCCTTCATGCCCTCTCTCTT
  CAGAGTATTAGTTGTGCTCGCTATGTGACCTCAGTCACTTACACAGTCTCTTTGAATTTTGGATTTATT
  GCATGTAAATGGAAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238518
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAAAARPRGRALGPVLPPTPLLLLVLRVLPACGATARDPGAAAGLSLHPTYFNLAEAARIWATATCGER
  GPGEGRPQPELYCKLVGGPTAPGSGHTIQGQFCDYCNSEDPRKAHPVTNAIDGSERWWQSPPLSSGTQY
  NRVNLTLDLGQLFHVAYILIKFANSPRPDLWVLERSVDFGSTYSPWQYFAHSKVDCLKEFGREANMAVT
  RDDDVLCVTEYSRIVPLENGEVVVSLINGRPGAKNFTFSHTLREFTKATNIRLRFLRTNTLLGHLISKA
  QRDPTVTRRYYYSIKDISIGGQCVCNGHAEVCNINNPEKLFRCECQHHTCGETCDRCCTGYNQRRWRPA
  AWEQSHECEACNCHGHASNCYYDPDVERQQASLNTQGIYAGGGVCINCQHNTAGVNCEQCAKGYYRPYG
  VPVDAPDGCIRKFHFKLVYLSLCVLPQRSHQANFGSVNNFLHALSLQSISCARYVTSVTYTVSLNFGFI
  ACKWK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302996
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001302996.2
• RefSeq Size: 1749 bp
• RefSeq ORF: 1467 bp
• Locus ID: 3909
• Cytogenetics: 18q11.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• MW: 54.6 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]'