beta IV Tubulin (TUBB4A) (NM_001289123) Human Tagged ORF Clone

CAT#: RG238562

TUBB4A (GFP-tagged) - Human tubulin, beta 4A class IVa (TUBB4A), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TUBB4A
• Synonyms: beta-5; DYT4; TUBB4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238562 representing NM_001289123.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGAAGGGGGGCTGCGGACCGAGAAACTGAGCGGCTCCCGGGGGCGCAGGGACCGTGCTCCGCCGTC
  TCCGCCGCATCTTCCACCCTCGCCGCCGCCGCAGCTCCCCGCGCTCGTGCCACCGCCGCCGCGTCCACC
  CTCAGCGCCACCGCCATGCGGGAGATCGTGCACCTGCAGGCCGGCCAGTGCGGCAACCAGATCGGGGCC
  AAGTTTTGGGAGGTTATCAGTGACGAACATGGCATCGACCCCACAGGCACATACCATGGGGACAGTGAC
  CTGCAACTGGAGAGGATCAACGTGTACTACAACGAGGCCACAGGAGGAAATTATGTCCCCAGAGCGGTG
  CTGGTGGACCTGGAACCCGGCACCATGGACTCTGTCCGTTCTGGCCCCTTCGGTCAGATCTTTCGGCCG
  GACAACTTCGTGTTTGGCCAATCCGGAGCCGGCAACAACTGGGCAAAGGGGCACTACACGGAGGGCGCA
  GAGCTGGTGGACGCTGTCCTGGACGTAGTCCGGAAGGAGGCCGAGAGCTGCGACTGCCTTCAGGGCTTC
  CAGCTGACCCACTCGCTGGGGGGTGGCACGGGGTCCGGAATGGGCACGCTGCTCATCAGTAAGATCCGC
  GAGGAGTTCCCAGACCGCATCATGAACACCTTCAGCGTGGTGCCCTCGCCCAAAGTGTCAGACACGGTG
  GTGGAGCCCTACAACGCCACGCTGTCTGTGCACCAGCTGGTGGAGAATACGGATGAGACCTACTGCATC
  GACAACGAGGCACTCTACGACATCTGTTTCCGCACCCTCAAGCTGACCACCCCCACCTACGGGGACCTC
  AACCACCTGGTGTCGGCCACCATGAGCGGGGTCACCACCTGCCTGCGCTTCCCGGGCCAGCTGAACGCC
  GACCTGCGCAAGCTGGCCGTCAACATGGTTCCCTTTCCTCGCCTGCACTTCTTCATGCCCGGCTTCGCA
  CCCCTGACCAGCCGGGGCAGCCAGCAGTACCGGGCCCTGACGGTGCCCGAGCTCACCCAGCAGATGTTC
  GATGCCAAGAACATGATGGCGGCGTGCGACCCGCGCCACGGCCGCTACCTGACCGTGGCCGCCGTGTTC
  CGGGGCCGCATGTCCATGAAGGAGGTGGACGAGCAGATGCTGAGCGTGCAGAGCAAGAACAGCAGCTAC
  TTCGTGGAGTGGATCCCCAACAACGTGAAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGATG
  GCCGCGACCTTCATCGGCAACAGCACGGCCATCCAGGAGCTGTTCAAGCGCATCTCCGAGCAGTTCACG
  GCCATGTTCCGGCGCAAGGCCTTCTTGCACTGGTACACGGGCGAGGGCATGGACGAGATGGAGTTCACC
  GAGGCCGAGAGCAACATGAATGACCTGGTATCTGAGTACCAGCAGTACCAGGACGCCACGGCCGAGGAG
  GGCGAGTTCGAGGAGGAGGCGGAGGAGGAGGTGGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238562
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRRGAADRETERLPGAQGPCSAVSAASSTLAAAAAPRARATAAASTLSATAMREIVHLQAGQCGNQIGA
  KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRP
  DNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIR
  EEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDL
  NHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMF
  DAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKM
  AATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEE
  GEFEEEAEEEVA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289123
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289123.1, NP_001276052.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1488 bp
• Locus ID: 10382
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Gap junction, Pathogenic Escherichia coli infection
• MW: 54.9 kDa
• Gene Summary: This gene encodes a member of the beta tubulin family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene cause hypomyelinating leukodystrophy-6 and autosomal dominant torsion dystonia-4. Alternate splicing results in multiple transcript variants encoding different isoforms. A pseudogene of this gene is found on chromosome X. [provided by RefSeq, Jan 2014]