WASF3 (NM_001291965) Human Tagged ORF Clone

CAT#: RG238581

WASF3 (GFP-tagged) - Human WAS protein family, member 3 (WASF3), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: WASF3
• Synonyms: Brush-1; SCAR3; WAVE3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238581 representing NM_001291965.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCTTTAGTGAAGAGGAACATTGAGCCCCGGCACTTGTGCCGGGGAGCTCTGCCTGAAGGGATTACC
  AGCGAACTTGAATGTGTAACCAATAGTACTCTTGCCGCTATCATACGCCAGCTGAGCAGTCTGAGCAAA
  CATGCTGAAGACATATTTGGTGAGTTGTTTAATGAGGCTAACAACTTCTACATCAGAGCAAATTCTCTT
  CAAGACAGAATTGATCGCCTTGCTGTCAAAGTCACCCAGCTGGATTCAACAGTGGAAGAGGTCTCACTA
  CAGGATATCAACATGAAAAAAGCTTTCAAAAGTTCCACAGTCCAAGACCAGCAAGTGGTTTCAAAGAAC
  AGCATTCCTAATCCTGTTGCTGATATTTACAACCAGAGTGATAAGCCACCGCCTCTGAACATCCTGACA
  CCATACAGAGATGACAAGAAGGATGGGCTGAAGTTCTATACTGATCCTTCCTATTTCTTTGACCTCTGG
  AAAGAAAAAATGCTACAGGACACAGAAGACAAAAGGAAAGAGAAAAGGCGTCAAAAGAGAGAGAAACAC
  AAGCTGAACCCTAACAGAAACCAGCAAGTAAATGTGAGAAAAGTCAGAACAAGAAAAGAAGAGTGGGAG
  AGAAGGAAAATGGGCATTGAGTTTATGAGTGACGCAAAGAAACTGGAGCAGGCAGGGAGCGCCAAAGAG
  GACAGAGTGCCCAGCGGGTCACATGCATCGGACGTTACGGATTACTCTTACCCGGCTACTCCCAACCAT
  TCTCTGCACCCCCAGCCTGTGACCCCTTCCTATGCAGCTGGTGACGTGCCACCACACGGGCCTGCAAGC
  CAGGCTGCGGAGCATGAGTACCGGCCCCCATCTGCCTCGGCGAGGCACATGGCCCTCAACAGACCTCAG
  CAGCCGCCCCCCCCGCCTCCCCCTCAGGCCCCAGAGGGGTCCCAGGCCTCTGCACCGATGGCTCCAGCA
  GACTACGGGATGCTCCCAGCGCAGATAATTGAGTATTACAACCCATCCGGACCACCTCCTCCGCCACCT
  CCTCCTGTGATTCCCTCAGCACAAACTGCCTTCGTCAGCCCTCTCCAGATGCCCATGCAGCCCCCGTTC
  CCTGCATCAGCCAGCTCCACGCACGCAGCTCCTCCTCACCCACCCTCCACCGGGCTCCTGGTCACAGCC
  CCGCCACCCCCGGGCCCACCACCTCCCCCGCCAGGCCCTCCTGGTCCCGGGTCTTCTCTTTCGTCCTCC
  CCAATGCATGGCCCCCCAGTAGCTGAGGCGAAGCGGCAAGAGCCTGCACAGCCACCAATCAGTGATGCT
  CGAAGCGACCTCCTCGCTGCTATTCGAATGGGAATTCAACTGAAAAAGGTGCAGGAGCAGCGGGAGCAG
  GAGGCCAAGCGGGAGCCAGTGGGGAATGACGTGGCCACGATCCTGTCCCGGCGCATTGCCGTGGAGTAC
  AGCGACTCTGACGACGACTCAGAGTTCGACGAGAACGACTGGTCCGAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238581
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPLVKRNIEPRHLCRGALPEGITSELECVTNSTLAAIIRQLSSLSKHAEDIFGELFNEANNFYIRANSL
  QDRIDRLAVKVTQLDSTVEEVSLQDINMKKAFKSSTVQDQQVVSKNSIPNPVADIYNQSDKPPPLNILT
  PYRDDKKDGLKFYTDPSYFFDLWKEKMLQDTEDKRKEKRRQKREKHKLNPNRNQQVNVRKVRTRKEEWE
  RRKMGIEFMSDAKKLEQAGSAKEDRVPSGSHASDVTDYSYPATPNHSLHPQPVTPSYAAGDVPPHGPAS
  QAAEHEYRPPSASARHMALNRPQQPPPPPPPQAPEGSQASAPMAPADYGMLPAQIIEYYNPSGPPPPPP
  PPVIPSAQTAFVSPLQMPMQPPFPASASSTHAAPPHPPSTGLLVTAPPPPGPPPPPPGPPGPGSSLSSS
  PMHGPPVAEAKRQEPAQPPISDARSDLLAAIRMGIQLKKVQEQREQEAKREPVGNDVATILSRRIAVEY
  SDSDDDSEFDENDWSD
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291965
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291965.1, NP_001278894.1
• RefSeq Size: 4841 bp
• RefSeq ORF: 1500 bp
• Locus ID: 10810
• Cytogenetics: 13q12.13
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Fc gamma R-mediated phagocytosis
• MW: 55.5 kDa
• Gene Summary: This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. A pseudogene of this gene have been defined on chromosome 6. Alternative splicing results in multiple transcript variants [provided by RefSeq, May 2014]