NADPH oxidase 4 (NOX4) (NM_001300995) Human Tagged ORF Clone

CAT#: RG238661

NOX4 (GFP-tagged) - Human NADPH oxidase 4 (NOX4), transcript variant 8

Specifications

Product Data

• Tag: TurboGFP
• Symbol: NOX4
• Synonyms: KOX; KOX-1; RENOX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238661 representing NM_001300995.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGCTGTATAACCAAGGGCCAGAGTATCACTACCTCCAC
  CAGATGTTGGGGCTAGGATTGTGTCTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCCTT
  ATCCTTTTACCCATGTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGA
  ACCAGGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCA
  GGCGTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTT
  GAACTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCTGGCCTG
  ACAGGGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATATGCAATAAGAGTTTCT
  AACTATGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTT
  TCAGGAGGGCTGCTGAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCGA
  ACCAGCTCTCAGAATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGA
  TTTTCAAAACCGGCAGAGTTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAA
  GCTAATTTTCCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTAC
  AGGTATATCCGGAGCAATAAGCCAGTCACCATCATTTCGGTCATGAGTCATCCCTCAGATGTCATGGAA
  ATCCGAATGGTCAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACTCTACATTGTCCCAGTGTA
  TCTGCATTAGAAAATCATCCATTTACCCTCACAATGTGTCCAACTGAAACCAAAGCAACATTTGGGGTT
  CATCTTAAAATAGTAGGAGACTGGACAGAACGATTTCGAGATTTACTACTGCCTCCATCTAGTCAAGAC
  TCCGAAATTCTGCCCTTCATTCAATCTAGAAATTATCCCAAGGATGACTGGAAACCATACAAGCTTAGA
  AGACTATACTTTATTTGGGTATGCAGAGATATCCAGTCCTTCCGTTGGTTTGCAGATTTACTCTGTATG
  TTGCATAACAAGTTTTGGCAAGAGAACAGACCTGACTATGTCAACATCCAGCTGTACCTCAGTCAAACA
  GATGGGATACAGAAGATAATTGGAGAAAAATATCATGCACTGAATTCAAGACTGTTTATAGGACGTCCT
  CGGTGGAAACTTTTGTTTGATGAAATAGCAAAATATAACAGAGGAAAAACAGTTGGTGTTTTCTGTTGT
  GGACCCAATTCACTATCCAAGACTCTTCATAAACTGAGTAACCAGAACAACTCATATGGGACAAGATTT
  GAATACAATAAAGAGTCTTTCAGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238661
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRR
  TRRLLDKSRTFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGL
  TGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNR
  TSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLY
  RYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCPTETKATFGV
  HLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCM
  LHNKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCC
  GPNSLSKTLHKLSNQNNSYGTRFEYNKESFS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300995
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300995.1, NP_001287924.1
• RefSeq Size: 4062 bp
• RefSeq ORF: 1545 bp
• Locus ID: 50507
• Cytogenetics: 11q14.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 60.4 kDa
• Gene Summary: This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]