CDC25B (NM_001287516) Human Tagged ORF Clone

CAT#: RG238677

CDC25B (GFP-tagged) - Human cell division cycle 25B (CDC25B), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CDC25B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238677 representing NM_001287516.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCACAGCGAAACCCCAAAGAGTCAGGTAGGGACCCTGCTCTTCCGCAGCCGCAGCCGCCTGACGCAC
  CTATCCCTGTCTCGACGGGCATCCGAATCCTCCCTGTCGTCTGAATCCTCCGAATCTTCTGATGCAGGT
  CTCTGCATGGATTCCCCCAGCCCTATGGACCCCCACATGGCGGAGCAGACGTTTGAACAGGCCATCCAG
  GCAGCCAGCCGGATCATTCGAAACGAGCAGTTTGCCATCAGACGCTTCCAGTCTATGCCGGTGAGGCTG
  CTGGGCCACAGCCCCGTGCTTCGGAACATCACCAACTCCCAGGCGCCCGACGGCCGGAGGAAGAGCGAG
  GCGGGCAGTGGAGCTGCCAGCAGCTCTGGGGAAGACAAGGAGAATGATGGATTTGTCTTCAAGATGCCA
  TGGAAGCCCACACATCCCAGCTCCACCCATGCTCTGGCAGAGTGGGCCAGCCGCAGGGAAGCCTTTGCC
  CAGAGACCCAGCTCGGCCCCCGACCTGATGTGTCTCAGTCCTGACCGGAAGATGGAAGTGGAGGAGCTC
  AGCCCCCTGGCCCTAGGTCGCTTCTCTCTGACCCCTGCAGAGGGGGATACTGAGGAAGATGATGGATTT
  GTGGACATCCTAGAGAGTGACTTAAAGGATGATGATGCAGTTCCCCCAGGCATGGAGAGTCTCATTAGT
  GCCCCACTGGTCAAGACCTTGGAAAAGGAAGAGGAAAAGGACCTCGTCATGTACAGCAAGTGCCAGCGG
  CTCTTCCGCTCTCCGTCCATGCCCTGCAGCGTGATCCGGCCCATCCTCAAGAGGCTGGAGCGGCCCCAG
  GACAGGGACACGCCCGTGCAGAATAAGCGGAGGCGGAGCGTGACCCCTCCTGAGGAGCAGCAGGAGGCT
  GAGGAACCTAAAGCCCGCGTCCTCCGCTCAAAATCACTGTGTCACGATGAGATCGAGAACCTCCTGGAC
  AGTGACCACCGAGAGCTGATTGGAGATTACTCTAAGGCCTTCCTCCTACAGACAGTAGACGGAAAGCAC
  CAAGACCTCAAGTACATCTCACCAGAAACGATGGTGGCCCTATTGACGGGCAAGTTCAGCAACATCGTG
  GATAAGTTTGTGATTGTAGACTGCAGATACCCCTATGAATATGAAGGCGGGCACATCAAGACTGCGGTG
  AACTTGCCCCTGGAACGCGACGCCGAGAGCTTCCTACTGAAGAGCCCCATCGCGCCCTGTAGCCTGGAC
  AAGAGAGTCATCCTCATTTTCCACTGTGAATTCTCATCTGAGCGTGGGCCCCGCATGTGCCGTTTCATC
  AGGGAACGAGACCGTGCTGTCAACGACTACCCCAGCCTCTACTACCCTGAGATGTATATCCTGAAAGGC
  GGCTACAAGGAGTTCTTCCCTCAGCACCCGAACTTCTGTGAACCCCAGGACTACCGGCCCATGAACCAC
  GAGGCCTTCAAGGATGAGCTAAAGACCTTCCGCCTCAAGACTCGCAGCTGGGCTGGGGAGCGGAGCCGG
  CGGGAGCTCTGTAGCCGGCTGCAGGACCAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238677
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MHSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQ
  AASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMP
  WKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGF
  VDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQ
  DRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKH
  QDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLD
  KRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNH
  EAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001287516
• ORF Size: 1548 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001287516.1, NP_001274445.1
• RefSeq Size: 3071 bp
• RefSeq ORF: 1551 bp
• Locus ID: 994
• Cytogenetics: 20p13
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, MAPK signaling pathway, Progesterone-mediated oocyte maturation
• MW: 59.3 kDa
• Gene Summary: 'CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]'