STIP1 (NM_001282653) Human Tagged ORF Clone

CAT#: RG238694

STIP1 (GFP-tagged) - Human stress-induced phosphoprotein 1 (STIP1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: STIP1
• Synonyms: HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238694 representing NM_001282653.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCGATGATGCC
  TTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCT
  GCCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCT
  GACTGGGGCAAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAG
  GCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGT
  GATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGAAACAAG
  CCTTCTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTC
  GATCTGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAG
  ACCAAGCCAGAGCCAATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTG
  GGGAACGATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTG
  GACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTACAATAAG
  TGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAGATTGCC
  AAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATCCATTTCTAT
  AACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTG
  AAGGAGCAAGAGCGGCTGGCCTACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAG
  TGTTTTCAGAAAGGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAA
  GATGCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCACTCAAG
  GACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCTGCGCTG
  GAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGCTGT
  AAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCCGAAGAT
  GTGAAGCGACGAGCCATGGCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATC
  CTGGAACAGATGCAGAAGGACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAG
  ATCCAGAAGCTGATGGATGTGGGTCTGATTGCAATTCGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238694
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKP
  DWGKGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNK
  PSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKEL
  GNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIA
  KAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNE
  CFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAAL
  EAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLI
  LEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282653
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282653.2
• RefSeq Size: 2147 bp
• RefSeq ORF: 1560 bp
• Locus ID: 10963
• Cytogenetics: 11q13.1
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Prion diseases
• MW: 60.2 kDa
• Gene Summary: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]). [supplied by OMIM, Jul 2009]