MIRO1 (RHOT1) (NM_001288758) Human Tagged ORF Clone

CAT#: RG238712

RHOT1 (GFP-tagged) - Human ras homolog family member T1 (RHOT1), transcript variant 7

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RHOT1
• Synonyms: ARHT1; MIRO-1; MIRO1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238712 representing NM_001288758.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGACCATCCTTCCTATTATGAACCAGTATACAGAAATAGAAACCTGTGTGGAGTGTTCAGCGAAA
  AACCTGAAGAACATATCAGAGCTCTTTTATTACGCACAGAAAGCTGTTCTTCATCCTACAGGGCCCCTG
  TACTGCCCAGAGGAGAAGGAGATGAAACCAGCTTGTATAAAAGCCCTTACTCGTATATTTAAAATATCT
  GATCAAGATAATGATGGTACTCTCAATGATGCTGAACTCAACTTCTTTCAGAGGATTTGTTTCAACACT
  CCATTAGCTCCTCAAGCTCTGGAGGATGTCAAGAATGTAGTCAGAAAACATATAAGTGATGGTGTGGCT
  GACAGTGGGTTGACCCTGAAAGGTTTTCTCTTTTTACACACACTTTTTATCCAGAGAGGGAGACACGAA
  ACTACTTGGACTGTGCTTCGACGATTTGGTTATGATGATGACCTGGATTTGACACCTGAATATTTGTTC
  CCCCTGCTGAAAATACCTCCTGATTGCACTACTGAATTAAATCATCATGCATATTTATTTCTCCAAAGC
  ACCTTTGACAAGCATGATTTGGATAGAGACTGTGCTTTGTCACCTGATGAGCTTAAAGATTTATTTAAA
  GTTTTCCCTTACATACCTTGGGGGCCAGATGTGAATAACACAGTTTGTACCAATGAAAGAGGCTGGATA
  ACCTACCAGGGATTCCTTTCCCAGTGGACGCTCACGACTTATTTAGATGTACAGCGGTGCCTGGAATAT
  TTGGGCTATCTAGGCTATTCAATATTGACTGAGCAAGAGTCTCAAGCTTCAGCTGTTACAGTGACAAGA
  GATAAAAAGATAGACCTGCAGAAAAAACAAACTCAAAGAAATGTGTTCAGATGTAATGTAATTGGAGTG
  AAAAACTGTGGGAAAAGTGGAGTTCTTCAGGCTCTTCTTGGAAGAAACTTAATGAGGCAGAAGAAAATT
  CGTGAAGATCATAAATCCTACTATGCGATTAACACTGTTTATGTATATGGACAAGAGAAATACTTGTTG
  TTGCATGATATCTCAGAATCGGAATTTCTAACTGAAGCTGAAATCATTTGTGATGTTGTATGCCTGGTA
  TATGATGTCAGCAATCCCAAATCCTTTGAATACTGTGCCAGGATTTTTAAGCAACACTTTATGGACAGC
  AGAATACCTTGCTTAATCGTAGCTGCAAAGTCAGACCTGCATGAAGTTAAACAAGAATACAGTATTTCA
  CCTACTGATTTCTGCAGGAAACACAAAATGCCTCCACCACAAGCCTTCACTTGCAATACTGCTGATGCC
  CCCAGTAAGGATATCTTTGTTAAATTGACAACAATGGCCATGTATCCAGAGGATCATTACAGAGACAGA
  CTCTCCCGAGACATGGGCCACACTGATAGAATAGAGAATTTGAGAAAAATCTGGGTCTTTCTAAAAACT
  GCTTTGCACGTGACACAAGCTGACCTCAAGAGCTCCACGTTTTGGCTTCGAGCAAGTTTTGGTGCTACT
  GTTTTTGCAGTTTTGGGCTTTGCTATGTACAAAGCATTATTGAAACAGCGA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238712
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  METILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKIS
  DQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHE
  TTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFK
  VFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTR
  DKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLL
  LHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSIS
  PTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPEDHYRDRLSRDMGHTDRIENLRKIWVFLKT
  ALHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288758
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288758.1, NP_001275687.1
• RefSeq Size: 3360 bp
• RefSeq ORF: 1572 bp
• Locus ID: 55288
• Cytogenetics: 17q11.2
• Protein Families: Transmembrane
• MW: 60.9 kDa