Complement factor 8 beta (C8B) (NM_001278544) Human Tagged ORF Clone

CAT#: RG238737

C8B (GFP-tagged) - Human complement component 8, beta polypeptide (C8B), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: C8B
• Synonyms: C82
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238737 representing NM_001278544.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACACTTGTATGACTCTGGCCTTCACTCTCTCTGGGCGTTTCTTCATGCTGCTTTCTCAGTACAGG
  TATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAA
  GACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGA
  AGGTGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAAC
  TGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGT
  GGGATAAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGC
  TCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACC
  CAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTTGAACGCAATGTCACAGAG
  AAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATACCTGGAATATTTGAACTTGGCATCAGT
  AGTCAAAGTGATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCTCTCATACTAAAAGCGTATTT
  CTGCATGCACGCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTAC
  GAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGT
  GATTTTGGGACCCACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATGAAC
  AAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAAAATGATTTTAAA
  ATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTAGGCAAATGCAGAGGTATTCTG
  AATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCCTGGTACGAGGAGGG
  GCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGA
  GACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCC
  ACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAA
  GTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGCTGTGAC
  TGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAGAATACCCCCATTGAT
  GGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAAGGCAGTGT
  AACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGACTGCTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238737
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDTCMTLAFTLSGRFFMLLSQYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVRCEGFVCAQTG
  RCVNRRLLCNGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPVLDHRYYAGGC
  SPHYILNTRFRKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFKIPGIFELGIS
  SQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEYSYGEYRDLFR
  DFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLGVSVGKCRGIL
  NEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVKVEPLYELVTA
  TDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACEVSYRKNTPID
  GKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278544
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278544.1, NP_001265473.1
• RefSeq Size: 2307 bp
• RefSeq ORF: 1590 bp
• Locus ID: 732
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 60.6 kDa
• Gene Summary: 'This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]'