EHD1 (NM_001282444) Human Tagged ORF Clone

CAT#: RG238761

EHD1 (GFP-tagged) - Human EH-domain containing 1 (EHD1), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: EHD1
• Synonyms: H-PAST; HPAST1; PAST; PAST1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238761 representing NM_001282444.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAG
  GGGCTGCGGCAGCTGTACGCGCAGAAGCTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCAC
  TCGCCCGCGCTGGAGGACGCTGACTTCGACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACG
  GGCAAGACCACCTTCATCCGACACCTGATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCCC
  ACCACCGACTCCTTCATCGCCGTCATGCACGGCCCCACTGAGGGCGTGGTGCCGGGCAACGCGCTCGTG
  GTGGACCCGCGGCGCCCCTTCCGCAAGCTCAACGCGTTTGGCAACGCTTTCCTCAACAGGTTCATGTGT
  GCCCAGCTGCCCAACCCCGTCCTGGACAGCATCAGCATCATCGACACCCCCGGGATCCTGTCTGGAGAG
  AAGCAGCGGATCAGCAGAGGCTATGACTTTGCAGCCGTCCTGGAGTGGTTCGCGGAGCGTGTGGACCGC
  ATCATCCTGCTCTTCGACGCCCACAAGCTGGACATCTCCGATGAGTTCTCGGAAGTGATCAAGGCTCTG
  AAGAACCATGAGGACAAGATCCGCGTGGTGCTGAACAAGGCAGACCAGATCGAGACGCAGCAGCTGATG
  CGGGTGTACGGGGCCCTCATGTGGTCCCTGGGCAAGATCATCAACACCCCCGAGGTGGTCAGGGTCTAC
  ATCGGCTCCTTCTGGTCCCACCCGCTCCTCATCCCCGACAACCGCAAGCTCTTTGAGGCCGAGGAGCAG
  GACCTCTTCAAGGACATCCAGTCACTGCCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAG
  CGGGCACGGCTGGCCAAGGTTCACGCCTACATCATCAGCTCCCTCAAGAAAGAGATGCCCAATGTCTTT
  GGTAAAGAGAGCAAAAAGAAAGAGCTGGTGAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAG
  CACCAGATCTCCCCTGGGGACTTCCCGAGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTC
  AGCAAGTTCCAGGCGCTGAAGCCCAAGCTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCG
  CGGCTGATGGTGATGGTGCGGCAGGAGGAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTT
  GACGGCACCATGAACGGGCCGTTCGGGCACGGCTACGGCGAGGGGGCCGGCGAGGGCATCGACGACGTG
  GAGTGGGTGGTGGGCAAGGACAAGCCCACCTACGACGAGATCTTCTACACGCTGTCCCCTGTCAACGGC
  AAGATCACGGGCGCCAACGCCAAGAAGGAGATGGTGAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAG
  ATCTGGAAGCTGGCCGACGTGGACAAGGACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCAC
  CTCATCAAGGTCAAGCTGGAGGGCCACGAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCC
  AAGCGCAGACATGAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238761
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYST
  GKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMC
  AQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKAL
  KNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQ
  DLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIERE
  HQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQVVKGGAF
  DGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGK
  IWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282444
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282444.1, NP_001269373.1
• RefSeq Size: 3676 bp
• RefSeq ORF: 1605 bp
• Locus ID: 10938
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 61.1 kDa
• Gene Summary: This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]