Complement factor 8 beta (C8B) (NM_001278543) Human Tagged ORF Clone

CAT#: RG238796

C8B (GFP-tagged) - Human complement component 8, beta polypeptide (C8B), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: C8B
• Synonyms: C82
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238796 representing NM_001278543.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCGGAGTGTGGATGTTACCCTGATGCCCATTGATTGTGAGCTGTCTAGTTGGTCCTCTTGGACCACA
  TGTGACCCCTGTCAGAAGAAAAGGTACAGGTATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAA
  CCGTGCAACTTCTCTGACAAGGAAGTCGAAGACTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGA
  TGTGAAGGCTTTGTGTGTGCACAGACAGGAAGGTGTGTAAACCGCAGACTTCTTTGCAATGGGGACAAT
  GACTGTGGAGACCAGTCAGATGAAGCAAACTGTAGAAGGATTTATAAAAAATGTCAGCATGAAATGGAC
  CAATACTGGGGAATTGGCAGTCTGGCCAGTGGGATAAATTTGTTCACAAACAGTTTTGAGGGCCCAGTT
  CTTGATCACAGGTATTATGCAGGTGGATGCTCCCCGCATTACATCCTGAACACGAGGTTTAGGAAGCCC
  TACAATGTGGAAAGCTACACGCCACAGACCCAAGGCAAATACGAATTCATATTAAAAGAGTATGAATCA
  TACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGCAAGCAAGTCTGGTTTCAGTTTTGGTTTTAAA
  ATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGTGATCGAGGCAAACACTATATTAGGAGAACC
  AAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCACGCTCTGACCTTGAAGTAGCACATTACAAG
  CTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCAGAGAGTTAAGCGGCTGCCCCTGGAGTAC
  AGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACCCACTACATCACAGAGGCTGTGCTTGGG
  GGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGGAGAGAGGAGATTATACTCTTAACAAC
  GTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCATTGAAGAGGTCTACGTCAGTCTGGGT
  GTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGACAGAAACAAGAGGGACACCATGGTG
  GAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCACCACCCTGGCATACCAGGAGCTG
  CCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAACCCAGCCATCATCAAAGTTAAG
  GTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCCAGCACAGTGAGGCAGAACATG
  AAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTGCTCCCTGCCAAGGAAATGGA
  GTCCCTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATCCCAAGGCCTAGCCTGTGAG
  GTCTCCTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCAAATTGGTCTTCATGCTCT
  GGAAGACGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATGGGGGTAGCCCCTGTTCA
  GGCCCTGCTTCAGAAACACTTGACTGCTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238796
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRSVDVTLMPIDCELSSWSSWTTCDPCQKKRYRYAYLLQPSQFHGEPCNFSDKEVEDCVTNRPCRSQVR
  CEGFVCAQTGRCVNRRLLCNGDNDCGDQSDEANCRRIYKKCQHEMDQYWGIGSLASGINLFTNSFEGPV
  LDHRYYAGGCSPHYILNTRFRKPYNVESYTPQTQGKYEFILKEYESYSDFERNVTEKMASKSGFSFGFK
  IPGIFELGISSQSDRGKHYIRRTKRFSHTKSVFLHARSDLEVAHYKLKPRSLMLHYEFLQRVKRLPLEY
  SYGEYRDLFRDFGTHYITEAVLGGIYEYTLVMNKEAMERGDYTLNNVHACAKNDFKIGGAIEEVYVSLG
  VSVGKCRGILNEIKDRNKRDTMVEDLVVLVRGGASEHITTLAYQELPTADLMQEWGDAVQYNPAIIKVK
  VEPLYELVTATDFAYSSTVRQNMKQALEEFQKEVSSCHCAPCQGNGVPVLKGSRCDCICPVGSQGLACE
  VSYRKNTPIDGKWNCWSNWSSCSGRRKTRQRQCNNPPPQNGGSPCSGPASETLDCS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278543
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278543.1, NP_001265472.1
• RefSeq Size: 2400 bp
• RefSeq ORF: 1620 bp
• Locus ID: 732
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 61.8 kDa
• Gene Summary: 'This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]'