EHD1 (NM_001282445) Human Tagged ORF Clone

CAT#: RG238847

EHD1 (GFP-tagged) - Human EH-domain containing 1 (EHD1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: EHD1
• Synonyms: H-PAST; HPAST1; PAST; PAST1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238847 representing NM_001282445.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAACAGCCAGGAACTGCAGCCAGCCCCGTGTCCGGCAGTATGTTCAGCTGGGTCAGCAAGGATGCC
  CGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAGAAG
  CTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCACTCGCCCGCGCTGGAGGACGCTGACTTC
  GACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACGGGCAAGACCACCTTCATCCGACACCTG
  ATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCCCACCACCGACTCCTTCATCGCCGTCATG
  CACGGCCCCACTGAGGGCGTGGTGCCGGGCAACGCGCTCGTGGTGGACCCGCGGCGCCCCTTCCGCAAG
  CTCAACGCGTTTGGCAACGCTTTCCTCAACAGGTTCATGTGTGCCCAGCTGCCCAACCCCGTCCTGGAC
  AGCATCAGCATCATCGACACCCCCGGGATCCTGTCTGGAGAGAAGCAGCGGATCAGCAGAGGCTATGAC
  TTTGCAGCCGTCCTGGAGTGGTTCGCGGAGCGTGTGGACCGCATCATCCTGCTCTTCGACGCCCACAAG
  CTGGACATCTCCGATGAGTTCTCGGAAGTGATCAAGGCTCTGAAGAACCATGAGGACAAGATCCGCGTG
  GTGCTGAACAAGGCAGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACGGGGCCCTCATGTGGTCC
  CTGGGCAAGATCATCAACACCCCCGAGGTGGTCAGGGTCTACATCGGCTCCTTCTGGTCCCACCCGCTC
  CTCATCCCCGACAACCGCAAGCTCTTTGAGGCCGAGGAGCAGGACCTCTTCAAGGACATCCAGTCACTG
  CCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAGCGGGCACGGCTGGCCAAGGTTCACGCC
  TACATCATCAGCTCCCTCAAGAAAGAGATGCCCAATGTCTTTGGTAAAGAGAGCAAAAAGAAAGAGCTG
  GTGAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAGCACCAGATCTCCCCTGGGGACTTCCCG
  AGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCGCTGAAGCCCAAG
  CTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCGCGGCTGATGGTGATGGTGCGGCAGGAG
  GAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTTGACGGCACCATGAACGGGCCGTTCGGG
  CACGGCTACGGCGAGGGGGCCGGCGAGGGCATCGACGACGTGGAGTGGGTGGTGGGCAAGGACAAGCCC
  ACCTACGACGAGATCTTCTACACGCTGTCCCCTGTCAACGGCAAGATCACGGGCGCCAACGCCAAGAAG
  GAGATGGTGAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAGATCTGGAAGCTGGCCGACGTGGACAAG
  GACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCTGGAGGGCCAC
  GAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAGACATGAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238847
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEQPGTAASPVSGSMFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADF
  DNKPMVLLVGQYSTGKTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRK
  LNAFGNAFLNRFMCAQLPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHK
  LDISDEFSEVIKALKNHEDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPL
  LIPDNRKLFEAEEQDLFKDIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKEL
  VNNLGEIYQKIEREHQISPGDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQE
  ESLMPSQVVKGGAFDGTMNGPFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKK
  EMVKSKLPNTVLGKIWKLADVDKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLVPPSKRRHE
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282445
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282445.1, NP_001269374.1
• RefSeq Size: 3354 bp
• RefSeq ORF: 1647 bp
• Locus ID: 10938
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 62.4 kDa
• Gene Summary: This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]