Trehalase (TREH) (NM_001301065) Human Tagged ORF Clone

CAT#: RG238869

TREH (GFP-tagged) - Human trehalase (brush-border membrane glycoprotein) (TREH), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TREH
• Synonyms: TRE; TREA; TREHD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238869 representing NM_001301065.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGGGAGGACCTGGGAGCTGTGCCTGCTACTGCTGCTGGGGCTGGGACTGGGGTCCCAGGAGGCC
  CTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAACCAAGTTCAAATGGCCAAG
  CTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCTATAGCTCCAGAACAAGTCCTGCAGACC
  TTCACTGAGCTGTCCAGGGACCACAATCACAGCATCCCCAGGGAGCAGCTGCAGGCGTTTGTCCACGAA
  CACTTCCAGGCCAAGGGGCAGGAGCTGCAGCCCTGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTC
  CTGCAGAAGATTTCAGATGCCAAACTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTG
  GGGAAGAAGATGAAGCCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACATCCC
  TTCATTGTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTGCTATGGGCATGTCCCCAATGGTGGGCGC
  GTGTACTACCTGCAGCGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTACTTGACTCACACC
  AATGACACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAATTGGACTTTTGGACCAAGAAC
  AGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTCCTGAATCGCTATTATGTCCCTTATGGGGGA
  CCCAGGCCTGAGTCCTACAGCAAAGATGTGGAGTTGGCTGACACCTTGCCAGAAGGAGACCGGGAGGCT
  CTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACGCTGGCTCATTGGAGGC
  CCAAACCCCAACTCGCTTAGCGGCATCCGAACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTA
  TGCCAAGCAGAGGAGCTGATGAGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACGAAGTAC
  AGAATCCTGCGGTCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAGCAGACCGGAGCCTGG
  TTCGATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTACCCATCCAACCTCACTCCACTCTGG
  GCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAGGCTCTGAAATACCTGGAGGACAACCGGATCCTG
  ACTTACCAGTATGGGATCCCGACCTCTCTCCAGAAGACAGGCCAGCAGTGGGATTTCCCCAATGCCTGG
  GCCCCCCTGCAGGACCTGGTCATCAGAGGCCTGGCCAAGGCACCTTTACGTCGGGCCCAGGAAGTGGCT
  TTCCAGCTGGCTCAGAATTGGATCCGAACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAG
  AAGTATGACGTCAGCAACGGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAGGGATTTGGC
  TGGACGAATGGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTGACCTCAGGGGCCAAGCTG
  GCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCCAGCCTCCTGCTCAGCCTCCTGCCATGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238869
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPGRTWELCLLLLLGLGLGSQEALPPPCESEIYCHGELLNQVQMAKLYQDDKQFVDMPLSIAPEQVLQT
  FTELSRDHNHSIPREQLQAFVHEHFQAKGQELQPWTPADWKDSPQFLQKISDAKLRAWAGQLHQLWKKL
  GKKMKPEVLSHPERFSLIYSEHPFIVPGGRFVEFYYCYGHVPNGGRVYYLQRSQPPLLTLMMDCYLTHT
  NDTAFLQENIETLALELDFWTKNRTVSVSLEGKNYLLNRYYVPYGGPRPESYSKDVELADTLPEGDREA
  LWAELKAGAESGWDFSSRWLIGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNFYSRLGNDSQATKY
  RILRSQRLAALNTVLWDEQTGAWFDYDLEKKKKNREFYPSNLTPLWAGCFSDPGVADKALKYLEDNRIL
  TYQYGIPTSLQKTGQQWDFPNAWAPLQDLVIRGLAKAPLRRAQEVAFQLAQNWIRTNFDVYSQKSAMYE
  KYDVSNGGQPGGGGEYEVQEGFGWTNGVVLMLLDRYGDRLTSGAKLAFLEPHCLAATLLPSLLLSLLPW
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301065
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301065.2
• RefSeq Size: 1781 bp
• RefSeq ORF: 1659 bp
• Locus ID: 11181
• Cytogenetics: 11q23.3
• Protein Pathways: Starch and sucrose metabolism
• MW: 63.4 kDa
• Gene Summary: This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]