CDK5RAP1 (NM_001278169) Human Tagged ORF Clone

CAT#: RG238897

CDK5RAP1 (GFP-tagged) - Human CDK5 regulatory subunit associated protein 1 (CDK5RAP1), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CDK5RAP1
• Synonyms: C20orf34; C42; CGI-05; HSPC167
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238897 representing NM_001278169.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCCGGCCTGAAGTAGCGGCGGAACGGAAGTCGCTTGTGTATGAACGCAGCGGCGGACCTGTGAGG
  GGATCCGACTTGCCGGCAGAACTTACGCTGCGGGACCCCGGGCACTGTTGCTGCTGCGGGAGTGCCTCA
  GCTCCTCAGGAGAAGCTGTCTTCAGAAGTGGAAGACCCACCTCCCTATCTCATGATGGATGAACTTCTT
  GGAAGGCAGAGAAAAGTCTACCTCGAGACCTATGGCTGCCAGATGAATGTGAATGACACAGAGATAGCC
  TGGTCCATCTTACAGAAGAGTGGCTACCTGCGGACCAGTAACCTCCAAGAGGCAGATGTGATTCTCCTT
  GTCACATGCTCTATCAGGGAGAAGGCTGAGCAGACCATCTGGAACCGTTTACATCAGCTTAAAGCCTTG
  AAGACAAGGCGGCCCCGCTCCCGGGTTCCTCTGAGGATTGGAATTCTAGGCTGCATGGCTGAGAGGTTG
  AAGGAGGAGATTCTCAACAGAGAGAAAATGGTAGATATTTTGGCTGGTCCTGATGCCTACCGGGACCTT
  CCCCGGCTGCTGGCTGTTGCTGAGTCGGGCCAGCAAGCTGCCAACGTGCTGCTCTCTCTGGACGAGACC
  TATGCTGATGTCATGCCAGTCCAGACAAGCGCCAGTGCCACGTCTGCCTTTGTGTCAATCATGCGAGGC
  TGTGACAACATGTGTAGCTACTGCATTGTTCCTTTCACCCGGGGCAGGGAGAGGAGTCGGCCTATTGCC
  TCCATTCTAGAGGAAGTGAAGAAGCTTTCTGAGCAGGGGCTGAAAGAAGTGACACTTCTTGGTCAGAAT
  GTTAATAGTTTTCGGGACAATTCGGAGGTCCAGTTCAACAGTGCAGTGCCTACCAATCTCAGTCGTGGC
  TTTACCACCAACTATAAAACCAAGCAAGGAGGACTTCGTTTTGCTCATCTTCTGGATCAGGTCTCCAGA
  GTAGATCCTGAAATGAGGATCCGTTTTACCTCTCCCCACCCCAAGGATTTTCCTGATGAGGTTCTGCAG
  CTGATTCATGAGAGAGATAACATCTGTAAACAGATCCACCTGCCAGCCCAGAGTGGAAGCAGCCGTGTG
  TTGGAGGCCATGCGGAGGGGATATTCAAGAGAAGCTTATGTGGAGTTAGTTCACCATATTAGAGAATCT
  ATTCCAGGTGTGAGCCTCAGCAGCGATTTCATTGCTGGCTTTTGTGGTGAGACGGAGGAAGATCACGTC
  CAGACAGTCTCTTTGCTCCGGGAAGTTCAGTACAACATGGGCTTCCTCTTTGCCTACAGCATGAGACAG
  AAGACACGGGCATATCATAGGCTGAAGGATGATGTCCCGGAAGAGGTAAAATTAAGGCGTTTGGAGGAA
  CTCATCACTATCTTCCGAGAAGAAGCAACAAAAGCCAATCAGACCTCTGTGGGCTGTACCCAGTTGGTG
  CTAGTGGAAGGGCTCAGTAAACGCTCTGCCACTGACCTGTGTGGCAGGAATGATGGAAACCTTAAGGTG
  ATCTTCCCTGATGCAGAGATGGAGGATGTCAATAACCCTGGGCTCAGGGTCAGAGCCCAGCCTGGGGAC
  TATGTGCTGGTGAAGATCACCTCAGCCAGTTCTCAGACACTTAGGGGACATGTTCTCTGCAGGACCACT
  CTGAGGGACTCTTCTGCATATTGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238897
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTRPEVAAERKSLVYERSGGPVRGSDLPAELTLRDPGHCCCCGSASAPQEKLSSEVEDPPPYLMMDELL
  GRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKAL
  KTRRPRSRVPLRIGILGCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDET
  YADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQN
  VNSFRDNSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRVDPEMRIRFTSPHPKDFPDEVLQ
  LIHERDNICKQIHLPAQSGSSRVLEAMRRGYSREAYVELVHHIRESIPGVSLSSDFIAGFCGETEEDHV
  QTVSLLREVQYNMGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITIFREEATKANQTSVGCTQLV
  LVEGLSKRSATDLCGRNDGNLKVIFPDAEMEDVNNPGLRVRAQPGDYVLVKITSASSQTLRGHVLCRTT
  LRDSSAYC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278169
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278169.1, NP_001265098.1
• RefSeq Size: 1911 bp
• RefSeq ORF: 1683 bp
• Locus ID: 51654
• Cytogenetics: 20q11.21
• MW: 63.4 kDa
• Gene Summary: This gene encodes a regulator of cyclin-dependent kinase 5 activity. This protein has also been reported to modify RNA by adding a methylthio-group and may thus have a dual function as an RNA methylthiotransferase and as an inhibitor of cyclin-dependent kinase 5 activity. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013]