CRNKL1 (NM_001278627) Human Tagged ORF Clone

CAT#: RG238911

CRNKL1 (GFP-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238911 representing NM_001278627.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCT
  CGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGG
  CAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAG
  CTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTT
  AAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTAT
  GAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTT
  GAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAA
  CAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATT
  GAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAATTAT
  GATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTAT
  GAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGG
  ATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCC
  TCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAA
  ATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAAC
  AAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTAT
  GAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATC
  CTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCA
  GAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTT
  TACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCT
  TCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAAC
  TGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACA
  GCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACT
  GATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAA
  CCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAG
  CACCATCCAGATGAGGACGTCGATGAGAGTGAATCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238911
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFE
  LRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKF
  EENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNY
  DAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQA
  SLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLY
  EKFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNL
  YRRLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGT
  ASDKERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAE
  HHPDEDVDESES
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278627
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278627.1, NP_001265556.1
• RefSeq Size: 4100 bp
• RefSeq ORF: 1695 bp
• Locus ID: 51340
• Cytogenetics: 20p11.23
• Protein Pathways: Spliceosome
• MW: 69.1 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]