CENTG3 (AGAP3) (NM_001281300) Human Tagged ORF Clone

CAT#: RG238973

AGAP3 (GFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AGAP3
• Synonyms: AGAP-3; CENTG3; cnt-g3; CRAG; MRIP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG238973 representing NM_001281300.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTGCTTGTGGGCACGCAGGATGCCATCAGCGCTGCGAATCCCCGGGTTATCGACGACAGCAGAGCC
  CGCAAGCTCTCCACAGATCTGAAGCGGTGCACCTACTATGAGACGTGCGCGACCTACGGGCTCAATGTG
  GAGCGTGTCTTCCAGGACGTGGCCCAGAAGGTAGTGGCCTTGCGAAAGAAGCAGCAACTGGCCATCGGG
  CCCTGCAAGTCACTGCCCAACTCGCCCAGCCACTCGGCCGTGTCCGCCGCCTCCATCCCGGCCGTGCAC
  ATCAACCAGGCCACGAATGGCGGCGGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCC
  AGCATCAGCCAGCGGGAGCTGCGCATCGAGACCATCGCTGCCTCCTCCACCCCCACACCCATCCGAAAG
  CAGTCCAAGCGGCGCTCCAACATCTTCACGTCTCGGAAGGGTGCTGACCTGGACCGGGAGAAGAAGGCT
  GCCGAGTGCAAGGTGGACAGCATCGGGAGCGGCCGCGCCATCCCCATCAAGCAGGGGATCCTGCTAAAG
  CGGAGCGGCAAGTCCCTGAACAAGGAGTGGAAGAAGAAGTATGTGACGCTCTGTGACAACGGGCTGCTC
  ACCTATCACCCCAGCCTGCATGATTACATGCAGAACATCCACGGCAAGGAGATTGACCTGCTGCGGACA
  ACGGTGAAAGTGCCAGGGAAGCGCCTGCCCCGAGCCACACCTGCCACAGCCCCGGGCACCAGCCCCCGT
  GCCAACGGGCTGTCCGTGGAGCGGAGTAACACACAGCTGGGTGGGGGCACAGAGGCAGAGGAGTCGTTT
  GAATTTGTGGTGGTGTCCCTCACTGGGCAGACGTGGCACTTCGAGGCTTCAACGGCGGAGGAGCGGGAG
  CTGTGGGTTCAGAGTGTGCAGGCCCAGATCCTTGCCAGCCTGCAAGGCTGCCGCAGTGCCAAGGACAAG
  ACTCGACTGGGGAACCAGAACGCAGCTCTGGCTGTGCAGGCCGTCCGCACCGTCCGCGGCAACAGCTTT
  TGTATCGACTGCGATGCACCCAATCCAGACTGGGCCAGCCTGAACCTGGGTGCCCTGATGTGCATTGAG
  TGCTCAGGCATCCACCGACACCTGGGGGCTCACCTGTCCCGGGTGCGCTCCCTTGACCTCGATGACTGG
  CCGCCTGAGCTGCTGGCTGTCATGACTGCCATGGGCAATGCCCTCGCCAACAGCGTCTGGGAGGGGGCC
  TTGGGTGGCTACTCCAAGCCAGGGCCTGATGCCTGCAGAGAGGAGAAGGAACGCTGGATACGGGCCAAG
  TATGAACAGAAGCTCTTCCTGGCCCCACTGCCAAGCTCAGATGTGCCACTGGGGCAGCAGCTGCTCCGG
  GCCGTGGTGGAAGATGACCTGCGGCTGTTGGTGATGCTCCTGGCACATGGCTCCAAAGAGGAGGTGAAT
  GAGACCTATGGGGACGGGGACGGGCGGACGGCTCTACATCTCTCCAGTGCCATGGCCAACGTTGTCTTC
  ACGCAGCTGCTCATCTGGTACGGGGTGGACGTGAGGAGCCGGGACGCCCGGGGCCTGACTCCACTGGCA
  TATGCTCGCCGGGCCGGCAGCCAGGAGTGTGCAGACATCTTGATCCAGCATGGCTGCCCTGGGGAGGGC
  TGTGGCTTAGCGCCTACCCCCAACAGAGAGCCTGCCAATGGCACCAACCCCTCTGCTGAGCTGCACCGT
  AGTCCTAGCCTCCTA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG238973
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQDVAQKVVALRKKQQLAIG
  PCKSLPNSPSHSAVSAASIPAVHINQATNGGGSAFSDYSSSVPSTPSISQRELRIETIAASSTPTPIRK
  QSKRRSNIFTSRKGADLDREKKAAECKVDSIGSGRAIPIKQGILLKRSGKSLNKEWKKKYVTLCDNGLL
  TYHPSLHDYMQNIHGKEIDLLRTTVKVPGKRLPRATPATAPGTSPRANGLSVERSNTQLGGGTEAEESF
  EFVVVSLTGQTWHFEASTAEERELWVQSVQAQILASLQGCRSAKDKTRLGNQNAALAVQAVRTVRGNSF
  CIDCDAPNPDWASLNLGALMCIECSGIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGA
  LGGYSKPGPDACREEKERWIRAKYEQKLFLAPLPSSDVPLGQQLLRAVVEDDLRLLVMLLAHGSKEEVN
  ETYGDGDGRTALHLSSAMANVVFTQLLIWYGVDVRSRDARGLTPLAYARRAGSQECADILIQHGCPGEG
  CGLAPTPNREPANGTNPSAELHRSPSLL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281300
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001281300.2
• RefSeq Size: 2783 bp
• RefSeq ORF: 1743 bp
• Locus ID: 116988
• Cytogenetics: 7q36.1
• MW: 63.3 kDa
• Gene Summary: This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017]