STIP1 (NM_001282652) Human Tagged ORF Clone

CAT#: RG239010

STIP1 (GFP-tagged) - Human stress-induced phosphoprotein 1 (STIP1), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: STIP1
• Synonyms: HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239010 representing NM_001282652.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGTCCGGCAGCCCAATGGGAGAGGTGGAAATTTCCAGAACGATCAGAACCAATGGGCGCGGCCAG
  CGCGGCTACGATTGGCAGTGCAAAAGACCAATCCGTGTCGCAGAAGTTCGCTCCTCCCTCCATTCGTGG
  AGCCTGAGATGGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCGATGAT
  GCCTTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGTACAGCAACCGTTCT
  GCTGCCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAG
  CCTGACTGGGGCAAGGGCTATTCACGAAAAGCAGCAGCTCTAGAGTTCTTAAACCGCTTTGAAGAAGCC
  AAGCGAACCTATGAGGAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAAT
  ATGGAGGCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTG
  GAGAGTGATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGA
  AACAAGCCTTCTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCGTCCTCCTT
  GGGGTCGATCTGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAA
  AAGGAGACCAAGCCAGAGCCAATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAA
  GAGCTGGGGAACGATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAG
  GAGCTGGACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTAC
  AATAAGTGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAG
  ATTGCCAAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATCCAT
  TTCTATAACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAA
  ATCCTGAAGGAGCAAGAGCGGCTGGCCTACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGC
  AACGAGTGTTTTCAGAAAGGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAAC
  CCGAAAGATGCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCA
  CTCAAGGACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCT
  GCGCTGGAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCC
  AGCTGTAAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCC
  GAAGATGTGAAGCGACGAGCCATGGCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGC
  CTTATCCTGGAACAGATGCAGAAGGACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCA
  CAGAAGATCCAGAAGCTGATGGATGTGGGTCTGATTGCAATTCGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239010
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MESGSPMGEVEISRTIRTNGRGQRGYDWQCKRPIRVAEVRSSLHSWSLRWVNELKEKGNKALSVGNIDD
  ALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEA
  KRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLR
  NKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEK
  ELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQ
  IAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKG
  NECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAA
  ALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMR
  LILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282652
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282652.1, NP_001269581.1
• RefSeq Size: 2766 bp
• RefSeq ORF: 1773 bp
• Locus ID: 10963
• Cytogenetics: 11q13.1
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Prion diseases
• MW: 68.5 kDa
• Gene Summary: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]). [supplied by OMIM, Jul 2009]