Metabotropic Glutamate Receptor 4 (GRM4) (NM_001282847) Human Tagged ORF Clone

CAT#: RG239059

GRM4 (GFP-tagged) - Human glutamate receptor, metabotropic 4 (GRM4), transcript variant 8

Specifications

Product Data

• Tag: TurboGFP
• Symbol: GRM4
• Synonyms: GPRC1D; mGlu4; MGLUR4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239059 representing NM_001282847.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCTCTGACAGCTGGGGCTCCAAGATTGCACCTGTGCTGCACCTGGAGGAGGTGGCTGAGGGTGCT
  GTCACGATCCTCCCCAAGAGGATGTCCGTACGAGGCTTCGACCGCTACTTCTCCAGCCGCACGCTGGAC
  AACAACCGGCGCAACATCTGGTTTGCCGAGTTCTGGGAGGACAACTTCCACTGCAAGCTGAGCCGCCAC
  GCCCTCAAGAAGGGCAGCCACGTCAAGAAGTGCACCAACCGTGAGCGAATTGGGCAGGATTCAGCTTAT
  GAGCAGGAGGGGAAGGTGCAGTTTGTGATCGATGCCGTGTACGCCATGGGCCACGCGCTGCACGCCATG
  CACCGTGACCTGTGTCCCGGCCGCGTGGGGCTCTGCCCGCGCATGGACCCTGTAGATGGCACCCAGCTG
  CTTAAGTACATCCGAAACGTCAACTTCTCAGGCATCGCAGGGAACCCTGTGACCTTCAATGAGAATGGA
  GATGCGCCTGGGCGCTATGACATCTACCAATACCAGCTGCGCAACGATTCTGCCGAGTACAAGGTCATT
  GGCTCCTGGACTGACCACCTGCACCTTAGAATAGAGCGGATGCACTGGCCGGGGAGCGGGCAGCAGCTG
  CCCCGCTCCATCTGCAGCCTGCCCTGCCAACCGGGTGAGCGGAAGAAGACAGTGAAGGGCATGCCTTGC
  TGCTGGCACTGCGAGCCTTGCACAGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACGTGTCCC
  TATGACATGCGGCCCACAGAGAACCGCACGGGCTGCCGGCCCATCCCCATCATCAAGCTTGAGTGGGGC
  TCGCCCTGGGCCGTGCTGCCCCTCTTCCTGGCCGTGGTGGGCATCGCTGCCACGTTGTTCGTGGTGATC
  ACCTTTGTGCGCTACAACGACACGCCCATCGTCAAGGCCTCGGGCCGTGAACTGAGCTACGTGCTGCTG
  GCAGGCATCTTCCTGTGCTATGCCACCACCTTCCTCATGATCGCTGAGCCCGACCTTGGCACCTGCTCG
  CTGCGCCGAATCTTCCTGGGACTAGGGATGAGCATCAGCTATGCAGCCCTGCTCACCAAGACCAACCGC
  ATCTACCGCATCTTCGAGCAGGGCAAGCGCTCGGTCAGTGCCCCACGCTTCATCAGCCCCGCCTCACAG
  CTGGCCATCACCTTCAGCCTCATCTCGCTGCAGCTGCTGGGCATCTGTGTGTGGTTTGTGGTGGACCCC
  TCCCACTCGGTGGTGGACTTCCAGGACCAGCGGACACTCGACCCCCGCTTCGCCAGGGGTGTGCTCAAG
  TGTGACATCTCGGACCTGTCGCTCATCTGCCTGCTGGGCTACAGCATGCTGCTCATGGTCACGTGCACC
  GTGTATGCCATCAAGACACGCGGCGTGCCCGAGACCTTCAATGAGGCCAAGCCCATTGGCTTCACCATG
  TACACCACTTGCATCGTCTGGCTGGCCTTCATCCCCATCTTCTTTGGCACCTCGCAGTCGGCCGACAAG
  CTGTACATCCAGACGACGACGCTGACGGTCTCGGTGAGTCTGAGCGCCTCGGTGTCCCTGGGAATGCTC
  TACATGCCCAAAGTCTACATCATCCTCTTCCACCCGGAGCAGAACGTGCCCAAGCGCAAGCGCAGCCTC
  AAAGCCGTCGTTACGGCGGCCACCATGTCCAACAAGTTCACGCAGAAGGGCAACTTCCGGCCCAACGGA
  GAGGCCAAGTCTGAGCTCTGCGAGAACCTTGAGGCCCCAGCGCTGGCCACCAAACAGACTTACGTCACT
  TACACCAACCATGCAATC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239059
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGSDSWGSKIAPVLHLEEVAEGAVTILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRH
  ALKKGSHVKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVDGTQL
  LKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLRIERMHWPGSGQQL
  PRSICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTCPYDMRPTENRTGCRPIPIIKLEWG
  SPWAVLPLFLAVVGIAATLFVVITFVRYNDTPIVKASGRELSYVLLAGIFLCYATTFLMIAEPDLGTCS
  LRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQLAITFSLISLQLLGICVWFVVDP
  SHSVVDFQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTM
  YTTCIVWLAFIPIFFGTSQSADKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSL
  KAVVTAATMSNKFTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNHAI
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282847
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282847.2
• RefSeq Size: 7217 bp
• RefSeq ORF: 1815 bp
• Locus ID: 2914
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction, Taste transduction
• MW: 68.4 kDa
• Gene Summary: 'L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]'