MCAK (KIF2C) (NM_001297657) Human Tagged ORF Clone

CAT#: RG239082

KIF2C (GFP-tagged) - Human kinesin family member 2C (KIF2C), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: KIF2C
• Synonyms: CT139; KNSL6; MCAK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239082 representing NM_001297657.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCACTGTCTCAGAGCTTCGCATCACGGCTCAGGAGAATGACATGGAGGTGGAGCTGCCTGCAGCT
  GCAAACTCCCGCAAGCAGTTTTCAGTTCCTCCTGCCCCCACTAGGCCTTCCTGCCCTGCAGTGGCTGAA
  ATACCATTGAGGATGGTCAGCGAGGAGATGGAAGAGCAAGTCCATTCCATCCGAGGCAGCTCTTCTGCA
  AACCCTGTGAACTCAGTTCGGAGGAAATCATGTCTTGTGAAGGAAGTGGAAAAAATGAAGAACAAGCGA
  GAAGAGAAGAAGGCCCAGAACTCTGAAATGAGAATGAAGAGAGCTCAGGAGTATGACAGTAGTTTTCCA
  AACTGGGAATTTGCCCGAATGATTAAAGAATTTCGGGCTACTTTGGAATGTCATCCACTTACTATGACT
  GATCCTATCGAAGAGCACAGAATATGTGTCTGTGTTAGGAAACGCCCACTGAATAAGCAAGAATTGGCC
  AAGAAAGAAATTGATGTGATTTCCATTCCTAGCAAGTGTCTCCTCTTGGTACATGAACCCAAGTTGAAA
  GTGGACTTAACAAAGTATCTGGAGAACCAAGCATTCTGCTTTGACTTTGCATTTGATGAAACAGCTTCG
  AATGAAGTTGTCTACAGGTTCACAGCAAGGCCACTGGTACAGACAATCTTTGAAGGTGGAAAAGCAACT
  TGTTTTGCATATGGCCAGACAGGAAGTGGCAAGACACATACTATGGGCGGAGACCTCTCTGGGAAAGCC
  CAGAATGCATCCAAAGGGATCTATGCCATGGCCTCCCGGGACGTCTTCCTCCTGAAGAATCAACCCTGC
  TACCGGAAGTTGGGCCTGGAAGTCTATGTGACATTCTTCGAGATCTACAATGGGAAGCTGTTTGACCTG
  CTCAACAAGAAGGCCAAGCTGCGCGTGCTGGAGGACGGCAAGCAACAGGTGCAAGTGGTGGGGCTGCAG
  GAGCATCTGGTTAACTCTGCTGATGATGTCATCAAGATGATCGACATGGGCAGCGCCTGCAGAACCTCT
  GGGCAGACATTTGCCAACTCCAATTCCTCCCGCTCCCACGCGTGCTTCCAAATTATTCTTCGAGCTAAA
  GGGAGAATGCATGGCAAGTTCTCTTTGGTAGATCTGGCAGGGAATGAGCGAGGCGCGGACACTTCCAGT
  GCTGACCGGCAGACCCGCATGGAGGGCGCAGAAATCAACAAGAGTCTCTTAGCCCTGAAGGAGTGCATC
  AGGGCCCTGGGACAGAACAAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACACAGGTGCTGAGGGAC
  TCCTTCATTGGGGAGAACTCTAGGACTTGCATGATTGCCACGATCTCACCAGGCATAAGCTCCTGTGAA
  TATACTTTAAACACCCTGAGATATGCAGACAGGGTCAAGGAGCTGAGCCCCCACAGTGGGCCCAGTGGA
  GAGCAGTTGATTCAAATGGAAACAGAAGAGATGGAAGCCTGCTCTAACGGGGCGCTGATTCCAGGCAAT
  TTATCCAAGGAAGAGGAGGAACTGTCTTCCCAGATGTCCAGCTTTAACGAAGCCATGACTCAGATCAGG
  GAGCTGGAGGAGAAGGCTATGGAAGAGCTCAAGGAGATCATACAGCAAGGACCAGACTGGCTTGAGCTC
  TCTGAGATGACCGAGCAGCCAGACTATGACCTGGAGACCTTTGTGAACAAAGCGGAATCTGCTCTGGCC
  CAGCAAGCCAAGCATTTCTCAGCCCTGCGAGATGTCATCAAGGCCTTGCGCCTGGCCATGCAGCTGGAA
  GAGCAGGCTAGCAGACAAATAAGCAGCAAGAAACGGCCCCAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239082
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSA
  NPVNSVRRKSCLVKEVEKMKNKREEKKAQNSEMRMKRAQEYDSSFPNWEFARMIKEFRATLECHPLTMT
  DPIEEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETAS
  NEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPC
  YRKLGLEVYVTFFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTS
  GQTFANSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECI
  RALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPSG
  EQLIQMETEEMEACSNGALIPGNLSKEEEELSSQMSSFNEAMTQIRELEEKAMEELKEIIQQGPDWLEL
  SEMTEQPDYDLETFVNKAESALAQQAKHFSALRDVIKALRLAMQLEEQASRQISSKKRPQ
  SGPTRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLL
  SHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGF
  PEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGP
  MFAFRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001297657
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001297657.2
• RefSeq Size: 2701 bp
• RefSeq ORF: 1839 bp
• Locus ID: 11004
• Cytogenetics: 1p34.1
• Protein Families: Druggable Genome
• MW: 69.3 kDa
• Gene Summary: This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]