PPWD1 (NM_001278926) Human Tagged ORF Clone

CAT#: RG239109

PPWD1 (GFP-tagged) - Human peptidylprolyl isomerase domain and WD repeat containing 1 (PPWD1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PPWD1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239109 representing NM_001278926.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGAGAACGAAGAGCGCTGGGTTGGACCTTTACCTGTGGAGGCAACACTGGCCAAGAAGAGGAAAG
  CAGCCACATGAAGTTGGAATTTCGAACTCTTATTTAGTCTTAGAGTTTGAAAGAGTCTATCTTGATAAT
  CTCCCCAGTGCATCCATGTATGAGCGCAGTTACATGCATAGAGATGTTATCACCCATGTGGTATGCACC
  AAAACAGATTTTATTATTACTGCCAGTCATGATGGACATGTCAAGTTCTGGAAAAAAATAGAAGAGGGA
  ATTGAATTTGTTAAACATTTTCGTAGTCACCTGGGAGTTATTGAGAGTATTGCAGTTAGCTCTGAGGGA
  GCATTGTTCTGTTCTGTGGGTGATGATAAAGCAATGAAGGTGTTTGATGTAGTGAACTTTGACATGATC
  AACATGCTGAAACTTGGCTATTTTCCTGGACAGTGTGAGTGGATCTATTGCCCAGGGGATGCAATTTCT
  TCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAAATTTTCATTTATGATGGCCGAGGAGATAACCAGCCA
  CTTCATATTTTTGACAAACTCCATACATCACCTCTTACTCAGATACGGCTAAACCCAGTTTACAAAGCA
  GTAGTGTCTTCTGACAAATCTGGGATGATTGAATACTGGACTGGGCCTCCTCATGAATATAAATTCCCC
  AAAAATGTGAACTGGGAATATAAAACTGACACTGATTTATATGAATTTGCCAAGTGTAAGGCTTATCCA
  ACCAGCGTATGTTTTTCACCAGATGGGAAGAAAATAGCTACTATTGGTTCTGATAGAAAAGTTAGAATT
  TTCAGATTTGTAACTGGAAAACTCATGAGAGTCTTTGATGAATCACTAAGCATGTTTACTGAACTGCAA
  CAGATGAGGCAACAGTTACCAGACATGGAATTTGGCCGACGAATGGCTGTAGAACGTGAGTTGGAGAAG
  GTTGATGCTGTAAGATTAATTAATATAGTTTTTGATGAAACTGGACACTTCGTGCTGTATGGAACAATG
  CTGGGCATTAAAGTTATAAATGTAGAGACAAACCGGTGTGTGCGGATTTTAGGCAAACAAGAAAATATT
  AGAGTGATGCAATTGGCTTTGTTCCAGGGGATAGCCAAAAAGCATCGTGCTGCAACTACTATAGAAATG
  AAAGCTTCTGAAAATCCTGTTCTTCAGAATATTCAAGCTGACCCAACAATAGTCTGTACATCATTCAAA
  AAGAATAGATTTTATATGTTTACCAAACGAGAACCAGAAGATACGAAAAGTGCAGATTCTGATCGAGAT
  GTTTTTAATGAGAAACCTTCTAAAGAAGAAGTCATGGCAGCTACTCAAGCTGAAGGACCTAAACGAGTT
  TCGGACAGTGCCATTATCCACACCAGCATGGGAGACATTCACACCAAACTTTTTCCTGTTGAGTGCCCT
  AAGACAGTGGAAAACTTCTGTGTTCACAGCAGAAATGGTTATTATAATGGGCATACATTTCACCGTATA
  ATTAAGGGCTTTATGATTCAGACTGGAGATCCAACAGGTACTGGTATGGGAGGAGAAAGCATATGGGGA
  GGAGAATTTGAAGATGAATTTCATTCAACATTACGACATGACAGGCCATACACACTCAGCATGGCTAAC
  GCGGGATCAAATACTAATGGATCCCAGTTTTTCATAACGGTAGTACCAACGCCTTGGCTTGATAATAAG
  CATACAGTATTTGGACGAGTGACTAAAGGAATGGAAGTTGTACAGAGGATCTCCAACGTCAAAGTCAAT
  CCCAAAACAGATAAGCCCTATGAGGATGTCAGCATCATAAATATTACTGTCAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239109
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRRTKSAGLDLYLWRQHWPRRGKQPHEVGISNSYLVLEFERVYLDNLPSASMYERSYMHRDVITHVVCT
  KTDFIITASHDGHVKFWKKIEEGIEFVKHFRSHLGVIESIAVSSEGALFCSVGDDKAMKVFDVVNFDMI
  NMLKLGYFPGQCEWIYCPGDAISSVAASEKSTGKIFIYDGRGDNQPLHIFDKLHTSPLTQIRLNPVYKA
  VVSSDKSGMIEYWTGPPHEYKFPKNVNWEYKTDTDLYEFAKCKAYPTSVCFSPDGKKIATIGSDRKVRI
  FRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVERELEKVDAVRLINIVFDETGHFVLYGTM
  LGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIEMKASENPVLQNIQADPTIVCTSFK
  KNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRVSDSAIIHTSMGDIHTKLFPVECP
  KTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPYTLSMAN
  AGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVSIINITVK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278926
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278926.1, NP_001265855.1
• RefSeq Size: 2216 bp
• RefSeq ORF: 1851 bp
• Locus ID: 23398
• Cytogenetics: 5q12.3
• MW: 70.7 kDa
• Gene Summary: Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. [UniProtKB/Swiss-Prot Function]