ABCB8 (NM_001282293) Human Tagged ORF Clone

CAT#: RG239147

ABCB8 (GFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 8 (ABCB8), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ABCB8
• Synonyms: EST328128; M-ABC1; MABC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239147 representing NM_001282293.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGGGTGAAACTGCTATTGCCGGCGGCTCCTGTTTTACCGCGTCAGCATGCTGGTGCATTTATTTCG
  GGTCGGGATTCGGGGTGGCCCATTCCCAGGCAGGCTGCTACCGCCCCTCCGCTTCCAGACATTCTCAGC
  TGTCAGCTGGCCTTGGGTGCGGCACTCGTGAATGTACAGATCCCCCTGCTCCTGGGCCAGCTGGTAGAG
  GTCGTGGCCAAGTACACAAGGGACCACGTAGGGAGTTTCATGACTGAGTCCCAGAATCTCAGCACCCAC
  CTGCTTATCCTCTATGGTGTCCAGGGACTGCTGACCTTCGGGTACCTGGTGCTGCTGTCCCACGTTGGC
  GAGCGCATGGCTGTGGACATGCGGAGGGCCCTCTTCAGCTCCCTGCTCCGACAAGACATCACCTTCTTT
  GACGCCAATAAGACAGGGCAGCTGGTGAGCCGCTTGACAACTGACGTGCAGGAGTTTAAGTCATCCTTC
  AAGCTTGTCATCTCCCAGGGGCTGCGAAGCTGCACCCAGGTGGCAGGCTGCCTGGTGTCCCTGTCCATG
  CTGTCGACACGCCTCACGCTGCTGCTGATGGTGGCCACACCAGCCCTGATGGGAGTGGGCACCCTGATG
  GGCTCAGGCCTCCGAAAATTGTCTCGCCAGTGTCAGGAGCAGATCGCCAGGGCAATGGGCGTAGCAGAC
  GAGGCCCTGGGCAATGTGCGGACTGTGCGTGCCTTCGCCATGGAGCAACGGGAAGAGGAGCGCTATGGG
  GCAGAGCTGGAAGCCTGCCGCTGCCGGGCAGAGGAGCTGGGCCGCGGCATCGCCTTGTTCCAAGGGCTT
  TCCAACATCGCCTTCAACTGCATGGTCTTGGGTACCCTATTTATTGGGGGCTCCCTTGTGGCCGGACAG
  CAGCTGACAGGGGGAGACCTCATGTCCTTCCTGGTGGCCTCCCAGACAGTGCAAAGGTCCATGGCCAAC
  CTCTCTGTCCTGTTTGGGCAGGTGGTCCGGGGGCTGAGTGCAGGTGCCCGGGTCTTTGAGTACATGGCC
  CTGAACCCCTGCATCCCACTGTCTGGGGGCTGCTGCGTCCCCAAAGAGCAGCTGCGTGGCTCCGTTACA
  TTTCAGAACGTCTGCTTCAGCTACCCCTGCCGCCCCGGCTTCGAGGTGCTGAAAGACTTCACCCTGACG
  CTGCCCCCTGGCAAGATCGTGGCCCTCGTGGGCCAGTCTGGCGGAGGAAAGACCACCGTGGCTTCCCTG
  CTGGAGCGCTTCTACGACCCCACGGCAGGCGTGGTGATGCTGGATGGGCGGGACCTGCGCACCCTTGAC
  CCCTCCTGGCTCCGGGGCCAGGTTGTCGGCTTCATCAGCCAGGAGCCCGTCCTGTTTGGGACGACCATC
  ATGGAAAACATCCGCTTTGGGAAGCTGGAAGCTTCCGATGAAGAGGTGTACACAGCCGCCCGGGAAGCG
  AATGCTCACGAGTTCATCACCAGCTTCCCCGAGGGCTACAACACGGTCGTCGGTGAACGGGGCACTACC
  CTGTCTGGGGGCCAGAAGCAGCGCCTGGCCATCGCCCGAGCCCTTATCAAGCAGCCCACGGTGCTGATA
  CTGGATGAAGCTACCAGCGCGCTGGATGCAGAGTCCGAGCGGGTTGTACAGGAGGCCCTGGACCGGGCC
  AGTGCAGGCCGCACGGTGCTGGTAATTGCCCACCGGCTCAGCACTGTCCGTGGGGCCCACTGCATTGTC
  GTCATGGCCGATGGCCGTGTCTGGGAGGCTGGGACACATGAAGAGCTCCTGAAGAAAGGCGGGCTATAC
  GCCGAGCTCATCCGGAGGCAGGCCCTGGATGCCCCGAGGACAGCGGCCCCACCGCCCAAAAAGCCAGAA
  GGCCCCAGGAGCCACCAGCACAAGTCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239147
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MRVKLLLPAAPVLPRQHAGAFISGRDSGWPIPRQAATAPPLPDILSCQLALGAALVNVQIPLLLGQLVE
  VVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFF
  DANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLM
  GSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGL
  SNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMA
  LNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASL
  LERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREA
  NAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRA
  SAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPE
  GPRSHQHKS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282293
• ORF Size: 1890 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282293.1, NP_001269222.1
• RefSeq Size: 4371 bp
• RefSeq ORF: 1893 bp
• Locus ID: 11194
• Cytogenetics: 7q36.1
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 68.5 kDa
• Gene Summary: This nuclear gene encodes a multi-pass membrane protein that is targeted to the mitochondrial inner membrane. The encoded protein is an ATP-dependent transporter that may mediate the passage of organic and inorganic molecules out of the mitochondria. Loss of function of the related gene in mouse results in a disruption of iron homeostasis between the mitochondria and cytosol. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]