SUV420h1 (KMT5B) (NM_001300908) Human Tagged ORF Clone

CAT#: RG239191

  • TrueORF®

SUV420H1 (GFP-tagged) - Human suppressor of variegation 4-20 homolog 1 (Drosophila) (SUV420H1), transcript variant 4


Reconstitution Protocol

USD 520.00

2 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Tag TurboGFP
Symbol KMT5B
Synonyms CGI-85; CGI85; MRD51; SUV420H1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG239191 representing NM_001300908.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTACTTAGACATGGAGAAAACGACTTCAGTGTCATGTACTCCACAAGGAAAAACTGTGCTCAACTC
TGGCTGGGTCCTGCTGCGTTTATAAACCATGATTGCAGACCTAATTGTAAGTTTGTGTCAACTGGTCGA
GATACAGCATGTGTGAAGGCTCTAAGAGACATTGAACCTGGAGAAGAAATTTCTTGTTATTATGGAGAT
GGGTTCTTTGGAGAAAATAATGAGTTCTGCGAGTGTTACACTTGCGAAAGACGGGGCACTGGTGCTTTT
AAATCCAGAGTGGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATATGGACTCAGAGAAACAGAT
AAACGTTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAATTCAGACAGTCAATCTGTCAGCTCT
AACACTGATGCAGATACCACTCAGGAAAAAAACAATGCAACTTCTAACCGAAAATCTTCAGTTGGCGTA
AAAAAGAATAGCAAGAGCAGAACGTTAACGAGGCAATCTATGTCAAGAATTCCAGCTTCTTCCAACTCT
ACCTCATCTAAGCTAACTCATATAAATAATTCCAGGGTACCAAAGAAACTGAAGAAGCCTGCAAAGCCT
TTACTTTCAAAGATAAAATTGAGAAATCATTGCAAGCGGCTGGAGCAAAAGAATGCTTCAAGAAAACTC
GAAATGGGAAACTTAGTACTGAAAGAGCCTAAAGTAGTTCTGTATAAAAATTTGCCCATTAAAAAAGAT
AAGGAGCCAGAGGGACCAGCCCAAGCCGCAGTTGCCAGCGGGTGCTTGACTAGACACGCGGCGAGAGAA
CACAGACAGAATCCTGTGAGAGGTGCTCATTCGCAGGGGGAGAGCTCGCCCTGCACCTACATAACTCGG
CGGTCAGTGAGGACAAGAACAAATCTGAAGGAGGCCTCTGACATCAAGCTTGAACCAAATACGTTGAAT
GGCTATAAAAGCAGTGTGACGGAACCTTGCCCCGACAGTGGTGAACAGCTGCAGCCAGCTCCTGTGCTG
CAGGAGGAAGAACTGGCTCATGAGACTGCACAAAAAGGGGAGGCAAAGTGTCATAAGAGTGACACAGGC
ATGTCCAAAAAGAAGTCACGACAAGGAAAACTTGTGAAACAGTTTGCAAAAATAGAGGAATCTACTCCA
GTGCACGATTCTCCTGGAAAAGACGACGCGGTACCAGATTTGATGGGTCCCCATTCTGACCAGGGTGAG
CACAGTGGCACTGTGGGCGTGCCTGTGAGCTACACAGACTGTGCTCCTTCACCCGTCGGTTGTTCAGTT
GTGACATCAGATAGCTTCAAAACAAAAGACAGCTTTAGAACTGCAAAAAGTAAAAAGAAGAGGCGAATC
ACAAGGTATGATGCACAGTTAATCCTAGAAAATAACTCTGGGATTCCCAAATTGACTCTTCGTAGGCGT
CATGATAGCAGCAGCAAAACAAATGACCAAGAGAATGATGGAATGAACTCTTCCAAAATAAGCATCAAG
TTAAGCAAAGACCATGACAACGATAACAATCTCTATGTAGCAAAGCTTAATAATGGATTTAACTCAGGA
TCAGGCAGTAGTTCTACAAAATTAAAAATCCAGCTAAAACGAGATGAGGAAAATAGGGGGTCTTATACA
GAGGGGCTTCATGAAAATGGGGTGTGCTGCAGTGATCCTCTTTCTCTCTTGGAGTCTCGAATGGAGGTG
GATGACTATAGTCAGTATGAGGAAGAAAGTACAGATGATTCCTCCTCTTCTGAGGGCGATGAAGAGGAG
GATGACTATGATGATGACTTTGAAGACGATTTTATTCCTCTTCCTCCAGCTAAGCGCTTGAGGTTAATA
GTTGGAAAAGACTCTATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAAT
GCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
>Peptide sequence encoded by RG239191
Blue=ORF Red=Cloning site Green=Tag(s)

MLLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGD
GFFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSDSQSVSS
NTDADTTQEKNNATSNRKSSVGVKKNSKSRTLTRQSMSRIPASSNSTSSKLTHINNSRVPKKLKKPAKP
LLSKIKLRNHCKRLEQKNASRKLEMGNLVLKEPKVVLYKNLPIKKDKEPEGPAQAAVASGCLTRHAARE
HRQNPVRGAHSQGESSPCTYITRRSVRTRTNLKEASDIKLEPNTLNGYKSSVTEPCPDSGEQLQPAPVL
QEEELAHETAQKGEAKCHKSDTGMSKKKSRQGKLVKQFAKIEESTPVHDSPGKDDAVPDLMGPHSDQGE
HSGTVGVPVSYTDCAPSPVGCSVVTSDSFKTKDSFRTAKSKKKRRITRYDAQLILENNSGIPKLTLRRR
HDSSSKTNDQENDGMNSSKISIKLSKDHDNDNNLYVAKLNNGFNSGSGSSSTKLKIQLKRDEENRGSYT
EGLHENGVCCSDPLSLLESRMEVDDYSQYEEESTDDSSSSEGDEEEDDYDDDFEDDFIPLPPAKRLRLI
VGKDSIDIDISSRRREDQSLRLNA

TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV

Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001300908
ORF Size 1935 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_001300908.2
RefSeq Size 5802 bp
RefSeq ORF 1938 bp
Locus ID 51111
Cytogenetics 11q13.2
Protein Families Druggable Genome
Protein Pathways Lysine degradation
MW 72 kDa
Gene Summary This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

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