DDX3Y (NM_001302552) Human Tagged ORF Clone

CAT#: RG239232

DDX3Y (GFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box helicase 3, Y-linked (DDX3Y), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DDX3Y
• Synonyms: DBY
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239232 representing NM_001302552.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAATTGGACGCCCGCGGAAAGACACCTGGAAGCTTGCTAATCTGGACCTGAACTCTGAAAAACAG
  AGTGGAGGAGCAAGTACAGCGAGCAAAGGGCGCTATATACCTCCTCACTTAAGGAACAGAGAAGCATCT
  AAAGGATTCCATGATAAAGACAGTTCAGGTTGGAGTTGCAGCAAAGATAAGGATGCATATAGCAGTTTT
  GGGTCTCGAGATTCTAGAGGAAAGCCTGGTTATTTCAGTGAACGTGGAAGTGGATCAAGGGGAAGATTT
  GATGATCGTGGACGGAGTGACTATGATGGTATTGGCAATCGTGAAAGACCTGGCTTTGGCAGATTTGAA
  CGGAGTGGACATAGTCGTTGGTGTGACAAGTCAGTTGAAGATGATTGGTCAAAACCACTTCCACCAAGT
  GAACGCTTGGAGCAAGAACTGTTTTCTGGAGGAAACACGGGGATTAACTTTGAGAAATATGATGATATA
  CCAGTAGAGGCAACCGGCAGTAACTGTCCTCCACATATTGAGAATTTTAGCGATATTGACATGGGAGAA
  ATTATCATGGGGAACATTGAACTTACTCGCTATACTCGTCCTACTCCAGTGCAAAAACATGCCATTCCT
  ATTATTAAGGGAAAAAGAGACTTAATGGCTTGTGCCCAAACAGGATCTGGGAAAACTGCAGCATTTCTT
  TTACCCATACTGAGTCAGATATATACAGATGGTCCAGGAGAAGCTTTGAAGGCTGTGAAGGAAAATGGA
  AGGTATGGGCGCCGCAAACAATATCCAATATCCTTGGTTTTAGCCCCAACAAGAGAATTGGCTGTACAG
  ATCTATGAGGAAGCCAGAAAATTTTCCTACCGATCTAGAGTTCGTCCTTGTGTAGTTTATGGTGGTGCT
  GATATTGGTCAGCAGATTCGGGACTTAGAACGTGGATGCCACTTGTTAGTAGCCACTCCAGGACGTCTA
  GTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTCTGCAAGTACTTAGTGTTGGATGAAGCTGAT
  AGGATGCTGGATATGGGATTTGAACCTCAGATACGTCGTATAGTTGAACAAGATACTATGCCACCAAAG
  GGCGTTCGTCACACCATGATGTTTAGTGCTACTTTTCCTAAGGAAATACAGATGCTTGCTCGTGACTTT
  TTGGATGAATATATCTTTTTGGCTGTAGGCAGAGTAGGCTCTACCTCTGAGAACATCACACAGAAAGTA
  GTTTGGGTGGAAGACTTAGATAAACGGTCATTTCTACTGGACATTTTAGGTGCAACAGGGAGTGATTCA
  CTTACTTTAGTGTTTGTGGAGACCAAAAAGGGAGCAGATTCCCTGGAGGATTTCTTATACCATGAAGGA
  TATGCTTGTACTAGTATTCATGGAGACCGGTCACAGAGAGATCGAGAGGAGGCCCTTCACCAGTTTCGC
  TCAGGAAAAAGCCCAATTCTAGTGGCTACAGCTGTGGCAGCACGAGGACTAGACATTTCAAATGTGAGA
  CATGTTATCAATTTTGATTTGCCAAGTGATATTGAAGAATATGTGCATCGTATTGGCCGTACAGGACGT
  GTAGGAAACCTGGGCCTTGCCACCTCATTCTTTAATGAAAAAAATATGAATATTACAAAGGATTTGTTG
  GATCTTCTTGTAGAAGCTAAACAAGAAGTGCCTTCTTGGTTGGAAAATATGGCTTATGAACACCACTAC
  AAGGGTGGCAGTCGTGGACGATCTAAAAGTAATAGATTCAGTGGAGGATTTGGTGCCAGAGACTATCGA
  CAAAGTAGTGGTTCCAGCAGTTCTGGCTTTGGTGCTAGTCGCGGAAGCAGCAGCCGCAGTGGTGGAGGT
  GGTTACGGCAACAGCAGAGGATTTGGTGGAGGTGGCTATGGAGGCTTCTACAATAGTGATGGATATGGA
  GGAAATTATAACTCCCAGGGGGTTGACTGGTGGGGCAAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239232
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEIGRPRKDTWKLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSF
  GSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPS
  ERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIP
  IIKGKRDLMACAQTGSGKTAAFLLPILSQIYTDGPGEALKAVKENGRYGRRKQYPISLVLAPTRELAVQ
  IYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEAD
  RMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKV
  VWVEDLDKRSFLLDILGATGSDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFR
  SGKSPILVATAVAARGLDISNVRHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNMNITKDLL
  DLLVEAKQEVPSWLENMAYEHHYKGGSRGRSKSNRFSGGFGARDYRQSSGSSSSGFGASRGSSSRSGGG
  GYGNSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302552
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001302552.2, NP_001289481.1
• RefSeq Size: 2470 bp
• RefSeq ORF: 1974 bp
• Locus ID: 8653
• Cytogenetics: Yq11.221
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 73.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]