CUL4A (NM_001278513) Human Tagged ORF Clone

CAT#: RG239240

CUL4A (GFP-tagged) - Human cullin 4A (CUL4A), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CUL4A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239240 representing NM_001278513.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTCTACAAGCAACTGCGTCAGGCCTGTGAAGACCACGTCCAGGCACAGATCCTTCCGTTTAGAGAA
  GACTCACTAGATAGTGTTTTATTTTTAAAGAAGATTAACACGTGCTGGCAGGACCACTGCAGACAAATG
  ATCATGATCAGAAGCATCTTCCTGTTCTTGGACCGCACCTATGTGCTGCAGAACTCCACGCTGCCCTCC
  ATCTGGGATATGGGATTAGAACTGTTTAGAACCCATATTATTAGTGATAAAATGGTTCAGAGTAAAACC
  ATTGATGGAATCCTACTGCTGATCGAGCGCGAGAGGAGCGGCGAGGCCGTGGACCGGAGCCTGTTGCGG
  AGCCTCCTGGGCATGCTGTCTGACCTGCAGGTGTATAAAGATTCATTTGAACTGAAATTTTTGGAAGAG
  ACTAATTGCTTATATGCTGCCGAAGGCCAAAGGTTAATGCAGGAAAGAGAGGTTCCAGAATATCTTAAC
  CATGTAAGTAAACGCTTAGAGGAAGAGGGAGACAGAGTAATCACTTACTTGGACCACAGCACACAGAAA
  CCACTGATTGCTTGTGTGGAGAAACAGCTATTAGGAGAACATTTAACAGCAATTCTGCAGAAAGGGCTC
  GACCACTTACTGGATGAGAACAGAGTGCCGGACCTCGCACAGATGTACCAGCTGTTCAGCCGGGTGAGG
  GGCGGGCAGCAGGCGCTGCTGCAGCACTGGAGCGAGTACATCAAGACTTTTGGAACAGCGATCGTAATC
  AATCCTGAGAAAGACAAAGACATGGTCCAAGACCTGTTGGACTTCAAGGACAAGGTGGACCACGTGATC
  GAGGTCTGCTTCCAGAAGAATGAGCGGTTCGTCAACCTGATGAAGGAGTCCTTTGAGACGTTCATCAAC
  AAGAGACCCAACAAGCCTGCAGAACTGATCGCAAAGCATGTGGATTCAAAGTTAAGAGCAGGCAACAAA
  GAAGCCACAGACGAGGAGCTGGAGCGGACGTTGGACAAGATCATGATCCTGTTCAGGTTTATCCACGGT
  AAAGATGTCTTTGAAGCATTTTATAAAAAAGATTTGGCAAAAAGACTCCTTGTTGGGAAAAGTGCCTCA
  GTCGATGCTGAAAAGTCTATGTTGTCAAAGCTCAAGCATGAGTGCGGTGCAGCCTTCACCAGCAAGCTG
  GAAGGCATGTTCAAGGACATGGAGCTTTCGAAGGACATCATGGTTCATTTCAAGCAGCATATGCAGAAT
  CAGAGTGACTCAGGCCCTATAGACCTCACAGTGAACATACTCACAATGGGCTACTGGCCAACATACACG
  CCCATGGAAGTGCACTTAACCCCAGAAATGATTAAACTTCAGGAAGTATTTAAGGCATTTTATCTTGGA
  AAGCACAGTGGTCGAAAACTTCAGTGGCAAACTACTTTGGGACATGCTGTTTTAAAAGCGGAGTTTAAA
  GAAGGGAAGAAGGAATTCCAGGTGTCCCTCTTCCAGACACTGGTGCTCCTCATGTTCAACGAGGGAGAT
  GGCTTCAGCTTTGAGGAGATAAAAATGGCCACGGGGATAGAGGATAGTGAATTGCGCAGAACGCTGCAG
  TCCCTGGCCTGTGGCAAAGCACGTGTGCTGATTAAAAGTCCCAAAGGAAAGGAAGTGGAAGATGGAGAC
  AAGTTCATTTTTAATGGAGAGTTCAAGCACAAGTTGTTTAGAATAAAGATCAATCAAATTCAGATGAAG
  GAAACTGTTGAGGAACAGGTTAGCACCACTGAGAGAGTGTTTCAGGATAGACAATATCAGATTGATGCT
  GCTATCGTCAGAATAATGAAGATGAGAAAGACTCTTGGTCATAATCTTCTAGTTTCTGAATTATATAAT
  CAGCTGAAATTTCCAGTAAAGCCTGGAGATTTGAAAAAGAGAATTGAATCTCTGATAGACAGAGACTAT
  ATGGAGAGAGACAAAGACAATCCGAATCAGTACCACTACGTGGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239240
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MLYKQLRQACEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPS
  IWDMGLELFRTHIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEE
  TNCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHLTAILQKGL
  DHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDKDMVQDLLDFKDKVDHVI
  EVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERTLDKIMILFRFIHG
  KDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQN
  QSDSGPIDLTVNILTMGYWPTYTPMEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFK
  EGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGD
  KFIFNGEFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYN
  QLKFPVKPGDLKKRIESLIDRDYMERDKDNPNQYHYVA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278513
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278513.2
• RefSeq Size: 4053 bp
• RefSeq ORF: 1980 bp
• Locus ID: 8451
• Cytogenetics: 13q34
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• MW: 77.3 kDa
• Gene Summary: CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins (Liu et al., 2009 [PubMed 19481525]). [supplied by OMIM, Oct 2009]