BRD2 (NM_001291986) Human Tagged ORF Clone

CAT#: RG239297

BRD2 (GFP-tagged) - Human bromodomain containing 2 (BRD2), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: BRD2
• Synonyms: BRD2-IT1; D6S113E; FSH; FSRG1; NAT; O27.1.1; RING3; RNF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239297 representing NM_001291986.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACATGGGTACTATTAAGAGGAGACTTGAAAACAATTATTATTGGGCTGCTTCAGAGTGTATGCAA
  GATTTTAATACCATGTTCACCAACTGTTACATTTACAACAAGCCCACTGATGATATTGTCCTAATGGCA
  CAAACGCTGGAAAAGATATTCCTACAGAAGGTTGCATCAATGCCACAAGAAGAACAAGAGCTGGTAGTG
  ACCATCCCTAAGAACAGCCACAAGAAGGGGGCCAAGTTGGCAGCGCTCCAGGGCAGTGTTACCAGTGCC
  CATCAGGTGCCTGCCGTCTCTTCTGTGTCACACACAGCCCTGTATACTCCTCCACCTGAGATACCTACC
  ACTGTCCTCAACATTCCCCACCCATCAGTCATTTCCTCTCCACTTCTCAAGTCCTTGCACTCTGCTGGA
  CCCCCGCTCCTTGCTGTTACTGCAGCTCCTCCAGCCCAGCCCCTTGCCAAGAAAAAAGGCGTAAAGCGG
  AAAGCAGATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTCCAGCTAGCCCTCCTGGG
  AGTCTTGAGCCTAAGGCAGCACGGCTTCCCCCTATGCGTAGAGAGAGTGGTCGCCCCATCAAGCCCCCA
  CGCAAAGACTTGCCTGACTCTCAGCAACAACACCAGAGCTCTAAGAAAGGAAAGCTTTCAGAACAGTTA
  AAACATTGCAATGGCATTTTGAAGGAGTTACTCTCTAAGAAGCATGCTGCCTATGCTTGGCCTTTCTAT
  AAACCAGTGGATGCTTCTGCACTTGGCCTGCATGACTACCATGACATCATTAAGCACCCCATGGACCTC
  AGCACTGTCAAGCGGAAGATGGAGAACCGTGATTACCGGGATGCACAGGAGTTTGCTGCTGATGTACGG
  CTTATGTTCTCCAACTGCTATAAGTACAATCCCCCAGATCACGATGTTGTGGCAATGGCACGAAAGCTA
  CAGGATGTATTTGAGTTCCGTTATGCCAAGATGCCAGATGAACCACTAGAACCAGGGCCTTTACCAGTC
  TCTACTGCCATGCCCCCTGGCTTGGCCAAATCGTCTTCAGAGTCCTCCAGTGAGGAAAGTAGCAGTGAG
  AGCTCCTCTGAGGAAGAGGAGGAGGAAGATGAGGAGGACGAGGAGGAAGAAGAGAGTGAAAGCTCAGAC
  TCAGAGGAAGAAAGGGCTCATCGCTTAGCAGAACTACAGGAACAGCTTCGGGCAGTACATGAACAACTG
  GCTGCTCTGTCCCAGGGTCCAATATCCAAGCCCAAGAGGAAAAGAGAGAAAAAAGAGAAAAAGAAGAAA
  CGGAAGGCAGAGAAGCATCGAGGCCGAGCTGGGGCCGATGAAGATGACAAGGGGCCTAGGGCACCCCGC
  CCACCTCAACCTAAGAAGTCCAAGAAAGCAAGTGGCAGTGGGGGTGGCAGTGCTGCTTTAGGCCCTTCT
  GGCTTTGGACCTTCTGGAGGAAGTGGCACCAAGCTCCCCAAAAAGGCCACAAAGACAGCCCCACCTGCC
  CTGCCTACAGGTTATGATTCAGAGGAGGAGGAAGAGAGCAGGCCCATGAGTTACGATGAGAAGCGGCAG
  CTGAGCCTGGACATCAACAAATTACCTGGGGAGAAGCTGGGCCGAGTTGTGCATATAATCCAAGCCAGG
  GAGCCCTCTTTACGTGATTCAAACCCAGAAGAGATTGAGATTGATTTTGAAACACTCAAGCCATCCACA
  CTTAGAGAGCTTGAGCGCTATGTCCTTTCCTGCCTACGTAAGAAACCCCGGAAGCCCTACACCATTAAG
  AAGCCTGTGGGAAAGACAAAGGAGGAACTGGCTTTGGAGAAAAAGCGGGAATTAGAAAAGCGGTTACAA
  GATGTCAGCGGACAGCTCAATTCTACTAAAAAGCCCCCCAAGAAAGCGAATGAGAAAACAGAGTCATCC
  TCTGCACAGCAAGTAGCAGTGTCACGCCTTAGCGCTTCCAGCTCCAGCTCAGATTCCAGCTCCTCCTCT
  TCCTCGTCGTCGTCTTCAGACACCAGTGATTCAGACTCAGGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239297
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQELVV
  TIPKNSHKKGAKLAALQGSVTSAHQVPAVSSVSHTALYTPPPEIPTTVLNIPHPSVISSPLLKSLHSAG
  PPLLAVTAAPPAQPLAKKKGVKRKADTTTPTPTAILAPGSPASPPGSLEPKAARLPPMRRESGRPIKPP
  RKDLPDSQQQHQSSKKGKLSEQLKHCNGILKELLSKKHAAYAWPFYKPVDASALGLHDYHDIIKHPMDL
  STVKRKMENRDYRDAQEFAADVRLMFSNCYKYNPPDHDVVAMARKLQDVFEFRYAKMPDEPLEPGPLPV
  STAMPPGLAKSSSESSSEESSSESSSEEEEEEDEEDEEEEESESSDSEEERAHRLAELQEQLRAVHEQL
  AALSQGPISKPKRKREKKEKKKKRKAEKHRGRAGADEDDKGPRAPRPPQPKKSKKASGSGGGSAALGPS
  GFGPSGGSGTKLPKKATKTAPPALPTGYDSEEEEESRPMSYDEKRQLSLDINKLPGEKLGRVVHIIQAR
  EPSLRDSNPEEIEIDFETLKPSTLRELERYVLSCLRKKPRKPYTIKKPVGKTKEELALEKKRELEKRLQ
  DVSGQLNSTKKPPKKANEKTESSSAQQVAVSRLSASSSSSDSSSSSSSSSSSDTSDSDSG
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291986
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001291986.2
• RefSeq Size: 4899 bp
• RefSeq ORF: 2046 bp
• Locus ID: 6046
• Cytogenetics: 6p21.32
• Protein Families: Protein Kinase
• MW: 75.3 kDa
• Gene Summary: 'This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. [provided by RefSeq, Dec 2010]'