CRNKL1 (NM_001278628) Human Tagged ORF Clone

CAT#: RG239306

CRNKL1 (GFP-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239306 representing NM_001278628.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCG
  GCTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCA
  CCTCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACT
  TTTGAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAA
  AGCCTAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATT
  ACACTCTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGG
  GACCGGGCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATG
  TTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCC
  TGGCACTCCTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGA
  TTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTAT
  TTTGCCCATGCACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCAC
  CTTTATGTTGCCTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAG
  TATGCCCTGGACAGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAG
  AAGTTTGGTGATAGGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAA
  GTGAAGGCGAATCCACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAA
  GCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCAC
  TGGAAGCGCTACATTTATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAG
  AGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATG
  TGGATACTGTATGCACAGTTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACT
  TCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAA
  TTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATT
  AAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATC
  AGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAA
  GAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATC
  AGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAA
  GAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGA
  AGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTC
  AAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATC
  TTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAG
  CAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGATGAGAGTGAATCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239306
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKT
  FEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIW
  DRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRARTIYER
  FVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYK
  YALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAE
  AEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKM
  WILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWI
  KFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWI
  SFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKV
  KKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278628
• ORF Size: 2061 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278628.2
• RefSeq Size: 4048 bp
• RefSeq ORF: 2064 bp
• Locus ID: 51340
• Cytogenetics: 20p11.23
• Protein Pathways: Spliceosome
• MW: 83.6 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]