CRNKL1 (NM_001278628) Human Tagged ORF Clone
CAT#: RG239306
- TrueORF®
CRNKL1 (GFP-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 5
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | CRNKL1 |
Synonyms | CLF; Clf1; CRN; HCRN; MSTP021; SYF3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239306 representing NM_001278628.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCG GCTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCA CCTCCACCTCAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACT TTTGAAGATAATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAA AGCCTAAAGGAGATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATT ACACTCTGGCTGAAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGG GACCGGGCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATG TTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCC TGGCACTCCTACATCAACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGA TTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTAT TTTGCCCATGCACGGAAAGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCAC CTTTATGTTGCCTTTGCCAAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAG TATGCCCTGGACAGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAG AAGTTTGGTGATAGGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAA GTGAAGGCGAATCCACACAATTATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAA GCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCAC TGGAAGCGCTACATTTATCTTTGGATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAG AGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATG TGGATACTGTATGCACAGTTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACT TCCATAGGCAAATGTCCAAAGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAA TTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATT AAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATC AGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAA GAAACAGAAAGAACACGAAACCTTTACCGGCGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATC AGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAA GAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGA AGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTC AAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCTGGGAAGAATACTTTGATTACATC TTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCCATGGCCAAACTGTGGAAGAAACAG CAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGATGAGGACGTCGATGAGAGTGAATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239306
Blue=ORF Red=Cloning site Green=Tag(s) MAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKT FEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIW DRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRARTIYER FVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYK YALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAE AEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKM WILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWI KFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWI SFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKV KKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278628 |
ORF Size | 2061 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001278628.2 |
RefSeq Size | 4048 bp |
RefSeq ORF | 2064 bp |
Locus ID | 51340 |
Cytogenetics | 20p11.23 |
Protein Pathways | Spliceosome |
MW | 83.6 kDa |
Gene Summary | The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013] |
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