TLE3 (NM_001282982) Human Tagged ORF Clone

CAT#: RG239345

TLE3 (GFP-tagged) - Human transducin-like enhancer of split 3 (TLE3), transcript variant 7

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239345 representing NM_001282982.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCTATGGCTTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTA
  GCACAGATCATGCCTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAG
  CAGGTCACCATGACGGAGCTGAACGCCATCATCGGGGTACGTGGACTCCCCAATCTGCCTCTTACCCAG
  CAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCACCCG
  TCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCGCC
  CTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAGA
  GAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCT
  GCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACAGCTTGAGCCGATACGACAGT
  GATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCCCGCAACGCCCCGGGTCAGC
  CCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAAAAAGATGCCCCCACCAGC
  CCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAACGACAAA
  TCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCCAACTCCAGGCACCAGC
  ACGACCCCAGGGCTCAGGTCGATGCCGGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTAT
  GCGGCGCCCTTCGCCATGATGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCC
  GGCCTCCACAACATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCG
  CCAATGGTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATT
  CCTGGAGGAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCC
  CACGACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAG
  GTGGTGTGTGCCGTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAG
  ATCTGGGACATCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAAT
  TACATCCGCTCCTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTC
  ACCATCTGGGACCTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGT
  TATGCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTC
  TGGGACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGAC
  ATCTCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGG
  GAGGGCCGACAGCTACAGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGG
  GAGTGGCTGGCTGTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTAC
  CAGCTGCACCTGCACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGC
  ACTGGGAAAGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAA
  TCCTCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAG
  AAGGCCACAGTTTATGAGGTCATCTAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239345
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPNLPLTQ
  QQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRER
  ESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVS
  PAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTS
  TTPGLRSMPASALRTPISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRS
  PMVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGE
  VVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTL
  TIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCID
  ISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKY
  QLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDK
  KATVYEVIY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282982
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282982.2
• RefSeq Size: 4491 bp
• RefSeq ORF: 2100 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Protein Families: Transcription Factors
• MW: 75.4 kDa
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'