Dynein intermediate chain 1 (DNAI1) (NM_001281428) Human Tagged ORF Clone
CAT#: RG239358
- TrueORF®
DNAI1 (GFP-tagged) - Human dynein, axonemal, intermediate chain 1 (DNAI1), transcript variant 2
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | DNAI1 |
Synonyms | CILD1; DIC1; ICS1; PCD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239358 representing NM_001281428.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGA ACCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAA GCCACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATT TTGACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAG CCTATTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCA GATGAAGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGTATCCTACCAAGGTTCTCAGGAGTCT GTCAAGGTGATTTCAGAAACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCT GGGAGTCAAACAGATGTGCCTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCT AAGCAGCCCAAGGAGAGAAAGCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTACAAC AACCCTGTCCGGGATCGAGAATGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAAT CAGTGGGAGATCTATGATGCCTATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAG GCAAAGACCCCAGTGGCTAAAAAATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAG ACTGATGATCTCATCAAATTGTCCCAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATAT GATGACATTGCTCAAGATTTTAAGTACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACC CTGCTGCCGCTCTGGAAGTTCCAAAATGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAAT CCAAAGTACAGGGATCTGTTTGCAGTGGGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATG CTGCTGCTCTACAGCCTGAAGAACCCCAGCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATG TGTCTCGACATCCACGTGGACCACCCCTACCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATT TACAACCTCAAGAAGCCCCACTCCCAGCCCTCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGAC CCTGTGTGGCAGGTCAAGTGGCAGAAGGATGACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCT GACGGCAGGATTGTGTCTTGGACTCTCGTGAAGAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAG GTGGAAGGCAGCACCACGGAAGTTCCTGAGGGGTTGCAGCTGCACCCAGTGGGTTGTGGCACTGCCTTT GACTTCCACAAAGAGATTGACTACATGTTCCTAGTGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCT AAATCCTACTCCAGCCAATTCCTCGACACCTATGACGCCCACAACATGTCAGTGGACACTGTGTCCTGG AACCCATACCACACCAAGGTCTTCATGTCCTGCAGCTCCGACTGGACAGTGAAGATCTGGGACCACACC ATCAAGACCCCGATGTTCATCTATGACCTGAACTCAGCCGTGGGTGATGTGGCCTGGGCGCCATACTCT TCTACTGTGTTCGCAGCAGTCACCACAGATGGGAAGGCCCACATATTTGACTTAGCCATCAACAAGTAT GAGGCCATCTGCAACCAGCCTGTGGCGGCCAAAAAGAACAGGCTCACCCACGTGCAGTTCAATCTCATC CACCCCATCATCATTGTGGGCGATGACCGTGGGCACATCATCAGCCTCAAGCTCTCACCCAATTTGCGC AAGATGCCAAAGGAAAAGAAGGGGCAGGAGGTGCAGAAGGGTCCAGCTGTGGAGATTGCGAAACTGGAC AAACTGCTGAACCTGGTGAGGGAAGTGAAAATCAAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239358
Blue=ORF Red=Cloning site Green=Tag(s) MIPASAKAPHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRI LTANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQVGNLIPKDSDEGRRQHYRDELVAVSYQGSQES VKVISETGNLEEDEEPKELETEPGSQTDVPAAGAAEKVTEEELMTPKQPKERKLTNQFNFSERASQTYN NPVRDRECQTEPPPRTNFSATANQWEIYDAYVEELEKQEKTKEKEKAKTPVAKKSGKMAMRKLTSMESQ TDDLIKLSQAAKIMERMVNQNTYDDIAQDFKYYDDAADEYRDQVGTLLPLWKFQNDKAKRLSVTALCWN PKYRDLFAVGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAI YNLKKPHSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLK VEGSTTEVPEGLQLHPVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSW NPYHTKVFMSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKY EAICNQPVAAKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLD KLLNLVREVKIKT TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001281428 |
ORF Size | 2109 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001281428.1, NP_001268357.1 |
RefSeq Size | 2605 bp |
RefSeq ORF | 2112 bp |
Locus ID | 27019 |
Cytogenetics | 9p13.3 |
Protein Families | Druggable Genome |
Protein Pathways | Huntington's disease |
MW | 80.2 kDa |
Gene Summary | This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
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