Dynein intermediate chain 1 (DNAI1) (NM_001281428) Human Tagged ORF Clone

CAT#: RG239358

DNAI1 (GFP-tagged) - Human dynein, axonemal, intermediate chain 1 (DNAI1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DNAI1
• Synonyms: CILD1; DIC1; ICS1; PCD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239358 representing NM_001281428.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATTCCTGCTTCTGCGAAGGCTCCCCATAAACAGCCTCATAAGCAGAGCATCAGCATAGGCAGAGGA
  ACCAGGAAGAGAGATGAAGATTCAGGGACTGAAGTGGGAGAAGGCACAGATGAATGGGCCCAATCCAAA
  GCCACAGTTAGACCCCCTGACCAGCTGGAGTTGACCGATGCGGAGTTAAAGGAGGAGTTCACTCGGATT
  TTGACAGCCAACAACCCACACGCACCCCAGAACATTGTCAGGTACAGCTTCAAAGAAGGCACATATAAG
  CCTATTGGCTTTGTGAACCAACTGGCAGTTCACTACACCCAGGTTGGGAACCTGATCCCCAAAGACTCA
  GATGAAGGACGGCGGCAGCATTACCGCGATGAATTAGTGGCAGTATCCTACCAAGGTTCTCAGGAGTCT
  GTCAAGGTGATTTCAGAAACAGGAAACCTCGAAGAAGACGAAGAGCCCAAGGAGTTAGAAACTGAGCCT
  GGGAGTCAAACAGATGTGCCTGCAGCTGGGGCAGCTGAAAAAGTGACTGAAGAAGAATTGATGACTCCT
  AAGCAGCCCAAGGAGAGAAAGCTCACTAACCAGTTCAACTTCAGTGAGAGGGCCTCACAGACCTACAAC
  AACCCTGTCCGGGATCGAGAATGCCAGACGGAGCCTCCTCCCAGGACAAACTTTTCAGCCACAGCCAAT
  CAGTGGGAGATCTATGATGCCTATGTAGAGGAACTTGAGAAGCAGGAAAAGACCAAAGAGAAGGAGAAG
  GCAAAGACCCCAGTGGCTAAAAAATCAGGGAAGATGGCCATGAGGAAGCTGACATCTATGGAGTCTCAG
  ACTGATGATCTCATCAAATTGTCCCAAGCTGCTAAGATCATGGAGCGGATGGTCAACCAGAATACATAT
  GATGACATTGCTCAAGATTTTAAGTACTATGACGATGCTGCTGATGAATACCGGGACCAGGTGGGTACC
  CTGCTGCCGCTCTGGAAGTTCCAAAATGACAAAGCCAAGCGCCTGTCCGTCACTGCCCTCTGCTGGAAT
  CCAAAGTACAGGGATCTGTTTGCAGTGGGATATGGCTCTTATGACTTCATGAAGCAGAGCCGGGGCATG
  CTGCTGCTCTACAGCCTGAAGAACCCCAGCTTCCCTGAGTACATGTTCAGCAGCAACAGCGGCGTCATG
  TGTCTCGACATCCACGTGGACCACCCCTACCTGGTGGCAGTAGGCCACTATGACGGCAACGTGGCCATT
  TACAACCTCAAGAAGCCCCACTCCCAGCCCTCCTTCTGCAGCTCAGCCAAGTCTGGCAAGCACTCAGAC
  CCTGTGTGGCAGGTCAAGTGGCAGAAGGATGACATGGACCAAAACCTTAACTTCTTCTCTGTGTCATCT
  GACGGCAGGATTGTGTCTTGGACTCTCGTGAAGAGAAAGCTGGTTCACATAGATGTCATCAAGCTGAAG
  GTGGAAGGCAGCACCACGGAAGTTCCTGAGGGGTTGCAGCTGCACCCAGTGGGTTGTGGCACTGCCTTT
  GACTTCCACAAAGAGATTGACTACATGTTCCTAGTGGGCACAGAGGAGGGAAAAATCTACAAGTGCTCT
  AAATCCTACTCCAGCCAATTCCTCGACACCTATGACGCCCACAACATGTCAGTGGACACTGTGTCCTGG
  AACCCATACCACACCAAGGTCTTCATGTCCTGCAGCTCCGACTGGACAGTGAAGATCTGGGACCACACC
  ATCAAGACCCCGATGTTCATCTATGACCTGAACTCAGCCGTGGGTGATGTGGCCTGGGCGCCATACTCT
  TCTACTGTGTTCGCAGCAGTCACCACAGATGGGAAGGCCCACATATTTGACTTAGCCATCAACAAGTAT
  GAGGCCATCTGCAACCAGCCTGTGGCGGCCAAAAAGAACAGGCTCACCCACGTGCAGTTCAATCTCATC
  CACCCCATCATCATTGTGGGCGATGACCGTGGGCACATCATCAGCCTCAAGCTCTCACCCAATTTGCGC
  AAGATGCCAAAGGAAAAGAAGGGGCAGGAGGTGCAGAAGGGTCCAGCTGTGGAGATTGCGAAACTGGAC
  AAACTGCTGAACCTGGTGAGGGAAGTGAAAATCAAGACC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239358
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MIPASAKAPHKQPHKQSISIGRGTRKRDEDSGTEVGEGTDEWAQSKATVRPPDQLELTDAELKEEFTRI
  LTANNPHAPQNIVRYSFKEGTYKPIGFVNQLAVHYTQVGNLIPKDSDEGRRQHYRDELVAVSYQGSQES
  VKVISETGNLEEDEEPKELETEPGSQTDVPAAGAAEKVTEEELMTPKQPKERKLTNQFNFSERASQTYN
  NPVRDRECQTEPPPRTNFSATANQWEIYDAYVEELEKQEKTKEKEKAKTPVAKKSGKMAMRKLTSMESQ
  TDDLIKLSQAAKIMERMVNQNTYDDIAQDFKYYDDAADEYRDQVGTLLPLWKFQNDKAKRLSVTALCWN
  PKYRDLFAVGYGSYDFMKQSRGMLLLYSLKNPSFPEYMFSSNSGVMCLDIHVDHPYLVAVGHYDGNVAI
  YNLKKPHSQPSFCSSAKSGKHSDPVWQVKWQKDDMDQNLNFFSVSSDGRIVSWTLVKRKLVHIDVIKLK
  VEGSTTEVPEGLQLHPVGCGTAFDFHKEIDYMFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMSVDTVSW
  NPYHTKVFMSCSSDWTVKIWDHTIKTPMFIYDLNSAVGDVAWAPYSSTVFAAVTTDGKAHIFDLAINKY
  EAICNQPVAAKKNRLTHVQFNLIHPIIIVGDDRGHIISLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLD
  KLLNLVREVKIKT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001281428
• ORF Size: 2109 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001281428.1, NP_001268357.1
• RefSeq Size: 2605 bp
• RefSeq ORF: 2112 bp
• Locus ID: 27019
• Cytogenetics: 9p13.3
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 80.2 kDa
• Gene Summary: This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]