SUV420h1 (KMT5B) (NM_001300907) Human Tagged ORF Clone

CAT#: RG239387

SUV420H1 (GFP-tagged) - Human suppressor of variegation 4-20 homolog 1 (Drosophila) (SUV420H1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: KMT5B
• Synonyms: CGI-85; CGI85; MRD51; SUV420H1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239387 representing NM_001300907.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGGAGAAATTATTCAAAGAACATGTATTTATTTATTTGCGAATGTTTGCAACTGACAGTGGATTT
  GAAATATTGCCATGTAATAGATACTCATCAGAACAAAATGGAGCCAAAATAGTTGCAACAAAAGAGTGG
  AAACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCCGAACTTTCAGAAATTGAGGAGAACATG
  CTACTTAGACATGGAGAAAACGACTTCAGTGTCATGTACTCCACAAGGAAAAACTGTGCTCAACTCTGG
  CTGGGTCCTGCTGCGTTTATAAACCATGATTGCAGACCTAATTGTAAGTTTGTGTCAACTGGTCGAGAT
  ACAGCATGTGTGAAGGCTCTAAGAGACATTGAACCTGGAGAAGAAATTTCTTGTTATTATGGAGATGGG
  TTCTTTGGAGAAAATAATGAGTTCTGCGAGTGTTACACTTGCGAAAGACGGGGCACTGGTGCTTTTAAA
  TCCAGAGTGGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATATGGACTCAGAGAAACAGATAAA
  CGTTTAAATAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAATTCAGACAGTCAATCTGTCAGCTCTAAC
  ACTGATGCAGATACCACTCAGGAAAAAAACAATGCAACTTCTAACCGAAAATCTTCAGTTGGCGTAAAA
  AAGAATAGCAAGAGCAGAACGTTAACGAGGCAATCTATGTCAAGAATTCCAGCTTCTTCCAACTCTACC
  TCATCTAAGCTAACTCATATAAATAATTCCAGGGTACCAAAGAAACTGAAGAAGCCTGCAAAGCCTTTA
  CTTTCAAAGATAAAATTGAGAAATCATTGCAAGCGGCTGGAGCAAAAGAATGCTTCAAGAAAACTCGAA
  ATGGGAAACTTAGTACTGAAAGAGCCTAAAGTAGTTCTGTATAAAAATTTGCCCATTAAAAAAGATAAG
  GAGCCAGAGGGACCAGCCCAAGCCGCAGTTGCCAGCGGGTGCTTGACTAGACACGCGGCGAGAGAACAC
  AGACAGAATCCTGTGAGAGGTGCTCATTCGCAGGGGGAGAGCTCGCCCTGCACCTACATAACTCGGCGG
  TCAGTGAGGACAAGAACAAATCTGAAGGAGGCCTCTGACATCAAGCTTGAACCAAATACGTTGAATGGC
  TATAAAAGCAGTGTGACGGAACCTTGCCCCGACAGTGGTGAACAGCTGCAGCCAGCTCCTGTGCTGCAG
  GAGGAAGAACTGGCTCATGAGACTGCACAAAAAGGGGAGGCAAAGTGTCATAAGAGTGACACAGGCATG
  TCCAAAAAGAAGTCACGACAAGGAAAACTTGTGAAACAGTTTGCAAAAATAGAGGAATCTACTCCAGTG
  CACGATTCTCCTGGAAAAGACGACGCGGTACCAGATTTGATGGGTCCCCATTCTGACCAGGGTGAGCAC
  AGTGGCACTGTGGGCGTGCCTGTGAGCTACACAGACTGTGCTCCTTCACCCGTCGGTTGTTCAGTTGTG
  ACATCAGATAGCTTCAAAACAAAAGACAGCTTTAGAACTGCAAAAAGTAAAAAGAAGAGGCGAATCACA
  AGGTATGATGCACAGTTAATCCTAGAAAATAACTCTGGGATTCCCAAATTGACTCTTCGTAGGCGTCAT
  GATAGCAGCAGCAAAACAAATGACCAAGAGAATGATGGAATGAACTCTTCCAAAATAAGCATCAAGTTA
  AGCAAAGACCATGACAACGATAACAATCTCTATGTAGCAAAGCTTAATAATGGATTTAACTCAGGATCA
  GGCAGTAGTTCTACAAAATTAAAAATCCAGCTAAAACGAGATGAGGAAAATAGGGGGTCTTATACAGAG
  GGGCTTCATGAAAATGGGGTGTGCTGCAGTGATCCTCTTTCTCTCTTGGAGTCTCGAATGGAGGTGGAT
  GACTATAGTCAGTATGAGGAAGAAAGTACAGATGATTCCTCCTCTTCTGAGGGCGATGAAGAGGAGGAT
  GACTATGATGATGACTTTGAAGACGATTTTATTCCTCTTCCTCCAGCTAAGCGCTTGAGGTTAATAGTT
  GGAAAAGACTCTATAGATATTGACATTTCTTCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAATGCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239387
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENM
  LLRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDG
  FFGENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSDSQSVSSN
  TDADTTQEKNNATSNRKSSVGVKKNSKSRTLTRQSMSRIPASSNSTSSKLTHINNSRVPKKLKKPAKPL
  LSKIKLRNHCKRLEQKNASRKLEMGNLVLKEPKVVLYKNLPIKKDKEPEGPAQAAVASGCLTRHAAREH
  RQNPVRGAHSQGESSPCTYITRRSVRTRTNLKEASDIKLEPNTLNGYKSSVTEPCPDSGEQLQPAPVLQ
  EEELAHETAQKGEAKCHKSDTGMSKKKSRQGKLVKQFAKIEESTPVHDSPGKDDAVPDLMGPHSDQGEH
  SGTVGVPVSYTDCAPSPVGCSVVTSDSFKTKDSFRTAKSKKKRRITRYDAQLILENNSGIPKLTLRRRH
  DSSSKTNDQENDGMNSSKISIKLSKDHDNDNNLYVAKLNNGFNSGSGSSSTKLKIQLKRDEENRGSYTE
  GLHENGVCCSDPLSLLESRMEVDDYSQYEEESTDDSSSSEGDEEEDDYDDDFEDDFIPLPPAKRLRLIV
  GKDSIDIDISSRRREDQSLRLNA
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300907
• ORF Size: 2139 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300907.1, NP_001287836.1
• RefSeq Size: 5835 bp
• RefSeq ORF: 2142 bp
• Locus ID: 51111
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 80 kDa
• Gene Summary: This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]