CGK2 (PRKG2) (NM_001282485) Human Tagged ORF Clone

CAT#: RG239441

PRKG2 (GFP-tagged) - Human protein kinase, cGMP-dependent, type II (PRKG2), transcript variant 6

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PRKG2
• Synonyms: cGK2; cGKII; PKG2; PRKGR2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239441 representing NM_001282485.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGAAATGGTTCAGTGAAACCTAAACATTCTAAGCACCCAGATGGACACTCTGGGAACCTCACCACT
  GATGCTCTGCGGAACAAGGTGACAGAGCTGGAGAGAGAGTTGAGGAGGAAGGATGCTGAGATCCAGGAG
  CGGGAGTACCATTTGAAGGAGCTGCGGGAGCAGCTGTCGAAGCAGACTGTGGCCATTGCTGAACTCACA
  GAGGAGCTCCAGAACAAGTGCATCCAGCTGAACAAGCTGCAGGATGTGGTGCATATGCAGGGAGGAAGC
  CCGCTTCAGGCCTCTCCAGATAAAGTGCCTCTTGAGGTCCACCGGAAGACCTCTGGATTGGTCTCTCTC
  CATAGCAGGAGGGGAGCAAAGGCTGGCGTGTCTGCTGAGCCAACAACCCGGACCTATGACCTGAACAAA
  CCCCCTGAATTTTCCTTTGAGAAAGCAAGAGTCAGAAAAGACTCCAGTGAGAAGAAGCTCATTACAGAT
  GCCCTTAATAAAAATCAGTTTCTGAAAAGACTGGATCCTCAGCAGATCAAAGACATGGTGGAATGCATG
  TATGGGAGAAACTATCAGCAAGGGAGTTACATTATTAAGCAAGGAGAACCAGGAAACCATATCTTTGTG
  CTGGCAGAGGGTCGACTAGAGGTGTTCCAAGGGGAGAAATTGCTGTCCTCCATCCCTATGTGGACCACA
  TTTGGGGAGCTTGCCATTTTATACAATTGTACAAGGACTGCCTCTGTGAAAGCTATTACCAATGTTAAA
  ACATGGGCACTAGATCGAGAGGTATTCCAGAATATAATGAGGAGGACAGCCCAAGCTAGAGATGAACAA
  TACAGAAACTTCCTCAGAAGTGTATCCTTGCTGAAGAATTTACCTGAAGATAAATTAACCAAGATCATT
  GACTGCTTGGAAGTGGAATACTATGACAAAGGAGATTACATCATTAGAGAGGGCGAGGAAGGAAGTACC
  TTTTTCATTTTGGCAAAAGGAAAGGTAAAAGTAACACAGAGCACAGAAGGCCATGATCAACCACAGCTG
  ATAAAAACACTGCAGAAAGGAGAATACTTTGGAGAAAAAGCTCTTATCAGTGATGATGTCAGGTCAGCT
  AACATTATTGCTGAAGAAAATGATGTTGCATGCCTGGTTATAGATCGAGAAACATTCAACCAAACTGTC
  GGTACATTTGAAGAGCTGCAAAAATACCTTGAAGGATATGTGGCAAACCTGAACCGTGATGATGAAAAA
  AGACATGCGAAGCGGTCCATGTCTAACTGGAAGCTGTCCAAAGCACTCTCTCTGGAAATGATTCAGCTG
  AAGGAGAAGGTTAAAGTAAAAAATGAGAATGTTGCTTTTGCTATGAAGTGTATAAGGAAGAAGCACATA
  GTTGACACCAAGCAGCAGGAGCATGTCTACTCAGAGAAGAGGATCCTAGAGGAGCTGTGCTCTCCATTC
  ATTGTGAAATTATATCGTACTTTCAAGGACAATAAGTATGTATACATGCTTCTGGAGGCCTGCTTAGGT
  GGGGAGCTCTGGAGTATATTAAGGGACAGAGGCAGCTTTGATGAACCCACCTCCAAATTCTGCGTTGCT
  TGTGTGACAGAAGCATTTGATTACCTGCATCGACTAGGTATTATCTACAGAGACTTGAAACCAGAAAAC
  TTAATTCTAGATGCTGAGGGTTACCTTAAATTGGTTGACTTTGGATTTGCGAAGAAAATAGGGTCTGGA
  CAGAAAACATGGACATTCTGTGGGACTCCAGAATATGTAGCTCCTGAAGTCATTCTCAACAAGGGACAT
  GACTTCAGTGTGGATTTCTGGTCACTGGGAATTCTAGTGTATGAGCTCCTAACGGGCAACCCACCCTTT
  TCTGGGGTTGACCAAATGATGACCTACAATTTGATTCTCAAAGGAATTGAAAAAATGGATTTTCCCAGG
  AAGATAACACGACGACCTGAGGATTTGATTCGGAGGCTTTGCAGGCAAAATCCAACAGAAAGGCTGGGA
  AATCTGAAGAATGGAATAAATGACATTAAGAAACACAGGTGGTTAAATGGTTTTAATTGGGAGGGACTG
  AAAGCACGGAGCCTTCCATCACCTTTGCAAAGAGAGCTCAAGGGACCCATAGATCACAGCTACTTTGAC
  AAATATCCTCCTGAAAAGGGAATGCCTCCAGATGAGCTATCAGGCTGGGATAAAGACTTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239441
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELT
  EELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNK
  PPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFV
  LAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQ
  YRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQL
  IKTLQKGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEK
  RHAKRSMSNWKLSKALSLEMIQLKEKVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSPF
  IVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPEN
  LILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPF
  SGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGL
  KARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282485
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282485.2
• RefSeq Size: 4735 bp
• RefSeq ORF: 2202 bp
• Locus ID: 5593
• Cytogenetics: 4q21.21
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction
• MW: 84.8 kDa
• Gene Summary: 'This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein binds to and inhibits the activation of several receptor tyrosine kinases. The membrane-bound protein is a regulator of intestinal secretion, bone growth and renin secretion. Alternate splicing results in multiple transcript variants encoding distinct isoforms whose regulatory N-termini differ in length but whose C-terminal catalytic domains are identical. [provided by RefSeq, May 2018]'