TLE2 (NM_001300846) Human Tagged ORF Clone

CAT#: RG239459

TLE2 (GFP-tagged) - Human transducin-like enhancer of split 2 (TLE2), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TLE2
• Synonyms: ESG; ESG2; GRG2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239459 representing NM_001300846.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTACCCCCAGGGAAGGCACCCGACCCCGCTCCAGTCCGGCCAGCCCTTCAAGTTCTCGATCTTGGAG
  ATCTGCGACCGCATCAAAGAAGAATTCCAGTTTCTTCAGGCTCAATACCACAGCCTCAAGCTAGAATGT
  GAGAAGCTGGCCAGCGAGAAGACGGAAATGCAGCGACATTATGTCATGTATTATGAGATGTCGTACGGG
  CTCAACATTGAAATGCATAAGCAGGCGGAGATTGTGAAGCGTCTGAGCGGTATCTGCGCTCAGATTATC
  CCCTTCCTGACCCAGGAGCATCAGCAGCAGGTGCTCCAGGCCGTAGAACGCGCCAAGCAGGTCACCGTG
  GGGGAGCTGAACAGCCTCATCGGGCAGCAGCAGCTCCAGCCGCTGTCCCACCACGCACCCCCTGTGCCC
  CTCACCCCCCGCCCAGCCGGGCTGGTGGGCGGCAGTGCTACGGGGCTGCTTGCTCTGTCTGGAGCCCTG
  GCTGCCCAGGCTCAGCTGGCGGCGGCTGTCAAGGAGGACCGTGCGGGCGTGGAGGCCGAGGGGTCCAGA
  GTGGAGAGAGCCCCGAGCAGGAGTGCATCTCCCTCGCCCCCTGAGAGTCTCGTGGAGGAGGAGCGACCG
  AGTGGCCCTGGTGGTGGCGGGAAGCAGAGAGCAGATGAGAAGGAGCCATCAGGACCTTATGAAAGCGAC
  GAAGACAAGAGTGATTACAATCTGGTGGTGGACGAGGACCAACCCTCAGAGCCCCCCAGCCCGGCTACC
  ACCCCCTGCGGAAAGGTACCCATCTGCATTCCTGCCCGTCGGGACCTGGTGGACAGTCCAGCCTCCTTG
  GCCTCTAGCCTTGGCTCACCGCTGCCTAGAGCCAAGGAGCTCATCCTGAATGACCTTCCCGCCAGCACT
  CCTGCCTCCAAATCCTGTGACTCCTCCCCGCCCCAGGACGCTTCCACCCCCGGGCCCAGCTCGGCCAGT
  CACCTCTGCCAGCTTGCTGCCAAGCCAGCACCTTCCACGGACAGCGTCGCCCTGAGGAGCCCCCTGACT
  CTGTCCAGTCCCTTCACCACGTCCTTCAGCCTGGGCTCCCACAGCACTCTCAACGGAGACCTCTCCGTG
  CCCAGCTCCTACGTCAGCCTCCACCTGTCCCCCCAGGTCAGCAGCTCTGTGGTGTACGGACGCTCCCCC
  GTGATGGCATTTGAGTCTCATCCCCATCTCCGAGGGTCATCCGTCTCTTCCTCCCTACCCAGCATCCCT
  GGGGGAAAGCCGGCCTACTCCTTCCACGTGTCTGCGGACGGGCAGATGCAGCCGGTTCCCTTCCCCTCG
  GATGCACTGGTAGGCGCGGGCATCCCGCGGCACGCCCGGCAGCTGCACACGCTGGCCCATGGCGAGGTG
  GTCTGCGCGGTCACCATCAGCGGCTCCACACAGCATGTGTACACGGGCGGCAAGGGCTGTGTGAAGGTG
  TGGGACGTGGGCCAGCCTGGGGCCAAGACGCCCGTGGCCCAGCTCGACTGCCTGAACCGAGACAACTAC
  ATTCGTTCCTGCAAGTTGCTGCCGGATGGCCGGAGTCTGATCGTGGGCGGTGAGGCCAGCACCTTGTCC
  ATTTGGGACCTGGCGGCGCCCACCCCCCGTATCAAGGCCGAGCTGACTTCCTCAGCCCCAGCCTGCTAC
  GCCCTGGCCGTCAGCCCCGACGCCAAGGTTTGCTTCTCCTGCTGCAGCGATGGCAACATTGTGGTCTGG
  GACCTGCAGAATCAGACTATGGTCAGGCAGTTCCAGGGCCACACGGACGGCGCCAGCTGCATTGATATT
  TCCGATTACGGCACTCGGCTCTGGACAGGGGGCCTGGACAACACGGTGCGCTGCTGGGACCTGCGGGAG
  GGCCGCCAGCTGCAGCAGCATGACTTCAGCTCCCAGATTTTCTCCCTGGGCCACTGCCCTAACCAGGAC
  TGGCTGGCGGTCGGAATGGAGAGTAGCAACGTGGAGATCCTGCACGTCCGCAAGCCGGAGAAATACCAG
  CTGCACCTCCACGAGAGCTGCGTGCTGTCCCTGAAGTTTGCCTCCTGCGGACGGTGGTTTGTGAGCACC
  GGGAAGGACAACCTGCTCAACGCCTGGAGGACGCCGTACGGGGCCAGCATTTTCCAGTCCAAGGAGTCG
  TCCTCAGTCCTGAGTTGTGACATCTCCAGAAATAACAAATACATCGTGACAGGCTCGGGGGACAAGAAG
  GCCACCGTGTATGAGGTGGTCTAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239459
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MYPQGRHPTPLQSGQPFKFSILEICDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG
  LNIEMHKQAEIVKRLSGICAQIIPFLTQEHQQQVLQAVERAKQVTVGELNSLIGQQQLQPLSHHAPPVP
  LTPRPAGLVGGSATGLLALSGALAAQAQLAAAVKEDRAGVEAEGSRVERAPSRSASPSPPESLVEEERP
  SGPGGGGKQRADEKEPSGPYESDEDKSDYNLVVDEDQPSEPPSPATTPCGKVPICIPARRDLVDSPASL
  ASSLGSPLPRAKELILNDLPASTPASKSCDSSPPQDASTPGPSSASHLCQLAAKPAPSTDSVALRSPLT
  LSSPFTTSFSLGSHSTLNGDLSVPSSYVSLHLSPQVSSSVVYGRSPVMAFESHPHLRGSSVSSSLPSIP
  GGKPAYSFHVSADGQMQPVPFPSDALVGAGIPRHARQLHTLAHGEVVCAVTISGSTQHVYTGGKGCVKV
  WDVGQPGAKTPVAQLDCLNRDNYIRSCKLLPDGRSLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACY
  ALAVSPDAKVCFSCCSDGNIVVWDLQNQTMVRQFQGHTDGASCIDISDYGTRLWTGGLDNTVRCWDLRE
  GRQLQQHDFSSQIFSLGHCPNQDWLAVGMESSNVEILHVRKPEKYQLHLHESCVLSLKFASCGRWFVST
  GKDNLLNAWRTPYGASIFQSKESSSVLSCDISRNNKYIVTGSGDKKATVYEVVY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300846
• ORF Size: 2232 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300846.2
• RefSeq Size: 2759 bp
• RefSeq ORF: 2235 bp
• Locus ID: 7089
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• MW: 80.4 kDa
• Gene Summary: ''