TPCN1 (NM_001301214) Human Tagged ORF Clone

CAT#: RG239469

TPCN1 (GFP-tagged) - Human two pore segment channel 1 (TPCN1), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TPCN1
• Synonyms: TPC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239469 representing NM_001301214.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATTACCAAGAGGCAGCAATCTACCTCCAGGAAGGCGAGAACAACGACAAGTTCTTCACCCACCCC
  AAGGATGCCAAGGCGCTGGCGGCCTACCTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCC
  ACGGCCCTGCTGCTGCTGCTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATC
  TATGTCCACGCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGC
  TGGCTGGGCCTCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTGGTGGTGCAG
  TTTGTCGAGGCCATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGC
  ATTTTCCTGGTGGACTGTCGGTATTGCGGTGGCGTCCGGCGCAACCTGCGGCAGATCTTCCAGTCCCTG
  CCGCCCTTCATGGACATCCTCCTGCTGCTGCTGTTCTTCATGATCATCTTTGCCATCCTCGGTTTCTAC
  TTGTTCTCCCCTAACCCTTCAGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTC
  CTTCTGACCACAGCCAATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTC
  TTCTTCATCGTGTACCTCTCCATCGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTCGAC
  ACCTTCAATGACATTGAGAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACCGCTATCCAGCAT
  GCCTACCGCCTGCTCATCAGCCAGAGGAGGCCTGCCGGCATCTCCTACAGGCAGTTTGAAGGCCTCATG
  CGCTTCTACAAGCCCCGGATGAGTGCCAGGGAGCGCTATCTTACCTTCAAGGCCCTGAATCAGAACAAC
  ACACCCCTGCTCAGCCTAAAGGACTTTTACGATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAG
  AAAAACAGAGAGCACTGGTTTGATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATC
  CTTGTGAAGTCCAAGGCCTTCCAGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTC
  GTGGAGACATTTATGCTGAAAGGTGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTCGTCTTT
  CTAACTATCTATGGGGTGGAGCTGTTCCTGAAGGTTGCCGGCCTGGGCCCTGTGGAGTACTTGTCTTCC
  GGATGGAACTTGTTTGACTTCTCCGTGACAGTGTTCGCCTTCCTGGGACTGCTGGCGCTGGCCCTCAAC
  ATGGAGCCCTTCTATTTCATCGTGGTCCTGCGCCCCCTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAG
  CGCTACCGCAACGTGCTGGACACCATGTTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTG
  CTCATCTTTTACTACTCCTTCGCCATCGTGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGC
  TGCAACACGAGTACAGTGGCAGATGCCTACCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTG
  GAGGAAGGCTACTATTATCTCAATAATTTTGACAACATCCTCAACAGCTTTGTGACCCTGTTTGAGCTC
  ACAGTTGTCAACAACTGGTACATCATCATGGAAGGCGTCACCTCTCAGACCTCCCACTGGAGCCGCCTC
  TACTTCATGACCTTTTACATTGTGACCATGGTGGTGATGACGATCATTGTCGCCTTTATCCTCGAGGCC
  TTCGTCTTCCGAATGAACTACAGCCGCAAGAACCAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAG
  AAGGAAATCTCCAAAGAAGAGCTGGTTGCCGTCCTGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCG
  GATGTCACCAGGCTGCTGGAGACCCTCTCCCAGATGGAGAGATACCAGCAACATTCCATGGTGTTTCTG
  GGACGGCGATCAAGGACCAAGAGCGACCTGAGCCTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTAT
  GAGGAGCATGCCAGGGAGCAAGAGCAGCAGCGACAACTCAGCAGCAGTGCAGCCCCCGCCGCCCAGCAG
  CCCCCAGGCAGCCGCCAGCGCTCCCAGACCGTTACC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239469
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGI
  YVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRC
  IFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFV
  LLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQH
  AYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAK
  KNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVF
  LTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKE
  RYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVV
  EEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEA
  FVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFL
  GRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301214
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301214.1, NP_001288143.1
• RefSeq Size: 5071 bp
• RefSeq ORF: 2247 bp
• Locus ID: 53373
• Cytogenetics: 12q24.13
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 87.8 kDa
• Gene Summary: Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]). [supplied by OMIM, Mar 2008]