RFX3 (NM_001282116) Human Tagged ORF Clone

CAT#: RG239472

RFX3 (GFP-tagged) - Human regulatory factor X, 3 (influences HLA class II expression) (RFX3), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RFX3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239472 representing NM_001282116.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGACATCAGAGACTGGGTCGGACACAGGCTCGACAGTGACCTTACAAACATCTGTGGCTAGTCAA
  GCAGCAGTGCCTACGCAGGTGGTACAGCAAGTACCAGTACAACAACAGGTACAGCAGGTACAGACTGTG
  CAGCAGGTACAACATGTCTATCCCGCTCAGGTGCAGTATGTGGAAGGAAGCGATACTGTCTATACCAAT
  GGAGCAATCCGAACAACAACGTATCCTTACACAGAGACACAGATGTACAGCCAAAATACTGGAGGGAAT
  TACTTTGATACTCAAGGGAGTTCCGCCCAGGTGACTACCGTGGTCTCATCCCACAGTATGGTGGGCACT
  GGTGGGATTCAGATGGGCGTCACAGGAGGACAACTCATCAGCAGCTCTGGAGGAACCTATCTGATCGGC
  AACTCAATGGAGAATTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCGACAATTGAAATG
  GCGATTGAGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTCAACAGCCATCTC
  CAGTGGCTGTTGGACAATTATGAGACAGCAGAAGGAGTGAGCCTTCCCAGAAGCACTCTGTACAACCAC
  TACCTTCGACACTGTCAGGAACACAAACTGGACCCAGTCAATGCTGCCTCTTTTGGAAAATTAATAAGA
  TCAATTTTTATGGGGCTACGAACCAGGAGATTGGGCACTAGAGGAAACTCCAAATACCACTACTATGGG
  ATTCGTGTCAAGCCAGATTCCCCTCTTAATCGTCTGCAAGAAGACATGCAGTATATGGCTATGAGACAA
  CAACCCATGCAACAGAAACAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACA
  GGAAGTGGTCAACAGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGCCAACATCATCAACAG
  TTTTTAGATGCATCTCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCTCTGCCAGATGGTACT
  ACCTTTGAGGATATCAAGTCACTGCAGAGTCTTTATAGAGAGCACTGTGAGGCAATATTGGACGTTGTT
  GTGAATCTTCAATTTAGCCTGATAGAAAAATTGTGGCAAACATTCTGGCGCTATTCTCCCTCTACTCCA
  ACTGATGGCACTACCATTACCGAATCGAGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAG
  CTGATAACTCTGTGCAAACATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAG
  GCTTTGGTGGAGATTCTCATCCCCGACGTCCTTAGACCTATTCCTAGTGCCTTGACCCAAGCCATTCGA
  AATTTTGCAAAAAGCCTTGAAGGTTGGCTTTCCAATGCCATGAACAATATTCCACAGAGAATGATACAA
  ACCAAGGTTGCCGCTGTAAGTGCCTTTGCCCAGACTCTGCGAAGATACACGTCGCTTAATCACCTGGCC
  CAGGCAGCTCGTGCAGTGCTTCAGAACACTTCCCAAATCAACCAGATGCTTAGTGACCTCAACCGTGTC
  GACTTTGCCAATGTCCAGGAGCAGGCTTCCTGGGTGTGCCAGTGTGATGACAACATGGTTCAGAGACTA
  GAAACAGACTTCAAGATGACTCTTCAGCAGCAGAGCACCCTGGAGCAGTGGGCTGCGTGGCTTGACAAT
  GTGATGATGCAAGCACTGAAACCCTATGAAGGAAGACCCAGTTTTCCTAAAGCCGCCAGGCAGTTTCTG
  CTAAAATGGTCTTTCTACAGCTCAATGGTTATTCGGGACTTAACCTTACGCAGTGCTGCTAGCTTTGGC
  TCCTTCCACCTGATCCGTCTACTCTACGACGAATATATGTTTTACTTAGTAGAACATCGTGTTGCTCAG
  GCAACAGGAGAGACTCCTATAGCAGTCATGGGCGAGTTTGGTGATTTAAATGCCGTGTCTCCTGGAAAT
  CTGGATAAAGATGAAGGCAGTGAAGTAGAAAGTGAAATGGATGAAGAACTGGATGACTCTTCAGAGCCT
  CAAGCCAAAAGAGAGAAAACAGAGCTGAGCCAGGCATTTCCAGTGGGCTGCATGCAGCCTGTTCTCGAG
  ACTGGCGTGCAACCAAGCCTCCTGAATCCAATTCACAGCGAGCACATTGTCACAAGTACTCAGACTATC
  AGACAGTGCAGCGCTACAGGAAATACCTACACTGCAGTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239472
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYVEGSDTVYTN
  GAIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQMGVTGGQLISSSGGTYLIG
  NSMENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNH
  YLRHCQEHKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQ
  QPMQQKQRYKPMQKVDGVADGFTGSGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGT
  TFEDIKSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESSNLSEIESRLPKAK
  LITLCKHESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQRMIQ
  TKVAAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCDDNMVQRL
  ETDFKMTLQQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFG
  SFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFGDLNAVSPGNLDKDEGSEVESEMDEELDDSSEP
  QAKREKTELSQAFPVGCMQPVLETGVQPSLLNPIHSEHIVTSTQTIRQCSATGNTYTAV
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282116
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282116.2
• RefSeq Size: 9271 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5991
• Cytogenetics: 9p24.2
• Protein Families: Transcription Factors
• MW: 83.5 kDa
• Gene Summary: 'This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]'