PSMD2 (NM_001278709) Human Tagged ORF Clone

CAT#: RG239475

PSMD2 (GFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: PSMD2
• Synonyms: P97; RPN1; S2; TRAP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239475 representing NM_001278709.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTATGTCAGGAGAAGTGGCTAAGGAGTGGCAGGAGCTGGATGACGCAGAGAAGGTCCAGCGGGAG
  CCTCTGCTCACTCTGGTGAAGGAAATCGTCCCCTATAACATGGCCCACAATGCAGAGCATGAGGCTTGC
  GACCTGCTTATGGAAATTGAGCAGGTGGACATGCTGGAGAAGGACATTGATGAAAATGCATATGCAAAG
  GTCTGCCTTTATCTCACCAGTTGTGTGAATTACGTGCCTGAGCCTGAGAACTCAGCCCTACTGCGTTGT
  GCCCTGGGTGTGTTCCGAAAGTTTAGCCGCTTCCCTGAAGCTCTGAGATTGGCATTGATGCTCAATGAC
  ATGGAGTTGGTAGAAGACATCTTCACCTCCTGCAAGGATGTGGTAGTACAGAAACAGATGGCATTCATG
  CTAGGCCGGCATGGGGTGTTCCTGGAGCTGAGTGAAGATGTCGAGGAGTATGAGGACCTGACAGAGATC
  ATGTCCAATGTACAGCTCAACAGCAACTTCTTGGCCTTAGCTCGGGAGCTGGACATCATGGAGCCCAAG
  GTGCCTGATGACATCTACAAAACCCACCTAGAGAACAACAGGTTTGGGGGCAGTGGCTCTCAGGTGGAC
  TCTGCCCGCATGAACCTGGCCTCCTCTTTTGTGAATGGCTTTGTGAATGCAGCTTTTGGCCAAGACAAG
  CTGCTAACAGATGATGGCAACAAATGGCTTTACAAGAACAAGGACCACGGAATGTTGAGTGCAGCTGCA
  TCTCTTGGGATGATTCTGCTGTGGGATGTGGATGGTGGCCTCACCCAGATTGACAAGTACCTGTACTCC
  TCTGAGGACTACATTAAGTCAGGAGCTCTTCTTGCCTGTGGCATAGTGAACTCTGGGGTCCGGAATGAG
  TGTGACCCTGCTCTGGCACTGCTCTCAGACTATGTTCTCCACAACAGCAACACCATGAGACTTGGTTCC
  ATCTTTGGGCTAGGCTTGGCTTATGCTGGCTCAAATCGTGAAGATGTCCTAACACTGCTGCTGCCTGTG
  ATGGGAGATTCAAAGTCCAGCATGGAGGTGGCAGGTGTCACAGCTTTAGCCTGTGGAATGATAGCAGTA
  GGGTCCTGCAATGGAGATGTAACTTCCACTATCCTTCAGACCATCATGGAGAAGTCAGAGACTGAGCTC
  AAGGATACTTATGCTCGTTGGCTTCCTCTTGGACTGGGTCTCAACCACCTGGGGAAGGGTGAGGCCATC
  GAGGCAATCCTGGCTGCACTGGAGGTTGTGTCAGAGCCATTCCGCAGTTTTGCCAACACACTGGTGGAT
  GTGTGTGCATATGCAGGCTCTGGGAATGTGCTGAAGGTGCAGCAGCTGCTCCACATTTGTAGCGAACAC
  TTTGACTCCAAAGAGAAGGAGGAAGACAAAGACAAGAAGGAAAAGAAAGACAAGGACAAGAAGGAAGCC
  CCTGCTGACATGGGAGCACATCAGGGAGTGGCTGTTCTGGGGATTGCCCTTATTGCTATGGGGGAGGAG
  ATTGGTGCAGAGATGGCATTACGAACCTTTGGCCACTTGCTGAGATATGGGGAGCCTACACTCCGGAGG
  GCTGTACCTTTAGCACTGGCCCTCATCTCTGTTTCAAATCCACGACTCAACATCCTGGATACCCTAAGC
  AAATTCTCTCATGATGCTGATCCAGAAGTTTCCTATAACTCCATTTTTGCCATGGGCATGGTGGGCAGT
  GGTACCAATAATGCCCGTCTGGCTGCAATGCTGCGCCAGTTAGCTCAATATCATGCCAAGGACCCAAAC
  AACCTCTTCATGGTGCGCTTGGCACAGGGCCTGACACATTTAGGGAAGGGCACCCTTACCCTCTGCCCC
  TACCACAGCGACCGGCAGCTTATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCTCTTTC
  CTGGATGTTCGAAACATTATTCTAGGCAAATCACACTATGTATTGTATGGGCTGGTGGCTGCCATGCAG
  CCCCGAATGCTGGTTACGTTTGATGAGGAGCTGCGGCCATTGCCAGTGTCTGTCCGTGTGGGCCAGGCA
  GTGGATGTGGTGGGCCAGGCTGGCAAGCCGAAGACTATCACAGGGTTCCAGACGCATACAACCCCAGTG
  TTGTTGGCCCACGGGGAACGGGCAGAATTGGCCACTGAGGAGTTTCTTCCTGTTACCCCCATTCTGGAA
  GGTTTTGTTATCCTTCGGAAGAACCCCAATTATGATCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239475
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSMSGEVAKEWQELDDAEKVQREPLLTLVKEIVPYNMAHNAEHEACDLLMEIEQVDMLEKDIDENAYAK
  VCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVEDIFTSCKDVVVQKQMAFM
  LGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYKTHLENNRFGGSGSQVD
  SARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLWDVDGGLTQIDKYLYS
  SEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYAGSNREDVLTLLLPV
  MGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPLGLGLNHLGKGEAI
  EAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKDKKEKKDKDKKEA
  PADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVSNPRLNILDTLS
  KFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTHLGKGTLTLCP
  YHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPLPVSVRVGQA
  VDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278709
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278709.2
• RefSeq Size: 2644 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5708
• Cytogenetics: 3q27.1
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• MW: 82.6 kDa
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]'