Semaphorin 3B (SEMA3B) (NM_001290061) Human Tagged ORF Clone

CAT#: RG239487

SEMA3B (GFP-tagged) - Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: SEMA3B
• Synonyms: LUCA-1; SemA; SEMA5; SEMAA; semaV
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239487 representing NM_001290061.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTCTGGGCAGTGGGGCTGGGGAGT
  GCCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAAGAGCTCCAGGCCTGGCATGGTCTCCAGACT
  TTCAGCCTGGAGCGAACCTGCTGCTACCAGGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTG
  GGTGCCGAGAACCATGTGGCCTCCCTCAACCTGGACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGG
  CCGGCCCCTGTGGAATGGCGAGAGGAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAAC
  TTCGTGAAGTTGCTGCATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTCCACCCA
  ACCTGTGCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTGGACCCAGGAAGGATA
  GAGGATGGCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCTGCCTCCGTGCTGGTGGGGGAGGAG
  CTATACTCAGGGGTGGCAGCAGACCTCATGGGACGAGACTTTACCATCTTTCGCAGCCTAGGGCAACGT
  CCAAGTCTCCGAACAGAGCCACACGACTCCCGCTGGCTCAATGAGCCCAAGTTTGTCAAGGTATTTTGG
  ATCCCGGAGAGCGAGAACCCAGACGACGACAAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCG
  GCGCCGGCACTGGGACGCCTGTCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAG
  CGCAGCCTGGTCAACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCCGGCGTCGAG
  GGCGACACCCACTTCGATCAGCTCCGCCCCTTCCCCGCAGAGGATGTGTTTCTGTTGTCCTCGCGGGAC
  CACCGGACCCCGCTGCTCTATGCCGTCTTCTCCACGTCCAGCAGCATCTTCCAGGGCTCTGCGGTGTGC
  GTGTACAGCATGAACGACGTGCGCCGGGCCTTCTTGGGACCCTTTGCACACAAGGAGGGGCCCATGCAC
  CAGTGGGTGTCATACCAGGGTCGCGTCCCCTACCCGCGGCCAGGCATGTGCCCCAGCAAGACCTTTGGC
  ACCTTCAGTTCCACCAAGGACTTCCCAGACGATGTCATCCAGTTTGCGCGGAACCACCCCCTCATGTAC
  AACTCTGTCCTGCCCACTGGGGGGCGCCCTCTTTTCCTACAAGTTGGAGCCAATTACACCTTCACTCAA
  ATTGCCGCGGACCGGGTTGCAGCCGCTGACGGACACTATGACGTCCTCTTCATTGGCACAGACGTTGGC
  ACGGTGCTGAAGGTGATCTCGGTCCCCAAGGGCAGTAGGCCCAGCGCAGAGGGGCTGCTCCTGGAGGAG
  CTGCACGTGTTTGAGGACTCGGCCGCTGTCACCAGCATGCAAATTTCTTCCAAGAGGCACCAGCTGTAC
  GTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGCACCGCTGCGCTGCCCACGGCCGCGTCTGCACC
  GAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGACGGGGTCGCGTGCACGCGCTTCCAGCCCAGT
  GCCAAGAGGCGGTTCCGGCGGCAAGACGTAAGGAATGGCGACCCCAGCACGTTGTGCTCCGGAGACTCG
  TCTCGTCCCGCGCTGCTGGAACACAAGGTGTTCGGCGTGGAGGGCAGCAGCGCCTTTCTGGAGTGTGAG
  CCCCGCTCGCTGCAGGCGCGCGTGGAGTGGACTTTCCAGCGCGCAGGGGTGACAGCCCACACCCAGGTG
  CTGGCAGAGGAGCGCACCGAGCGCACCGCCCGGGGACTACTGCTGCGCAGGCTGCGGCGCCGGGACTCG
  GGCGTGTACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAACCGCTGCGTCGCCTGTCGCTGCACGTG
  TTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGCTGCGCCCGCCGCGCCGCCGGGCCCC
  AAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCGGGCGGAGGTGGCAGCGCGAACTCCCTGCGC
  ATGTGCCGCCCGCAGCCTGCGCTGCAGTCACTGCCCCTGGAGTCGCGGAGAAAGGGCCGTAACCGGAGG
  ACCCACGCCCCTGAGCCTCGCGCTGAGCGGGGGCCGCGCAGCGCAACGCACTGG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239487
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGLQTFSLERTCCYQALLVDEERGRLFV
  GAENHVASLNLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLLHAYNRTHLLACGTGAFHP
  TCAFVEVGHRAEEPVLRLDPGRIEDGKGKSPYDPRHRAASVLVGEELYSGVAADLMGRDFTIFRSLGQR
  PSLRTEPHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQ
  RSLVNKWTTFLKARLVCSVPGVEGDTHFDQLRPFPAEDVFLLSSRDHRTPLLYAVFSTSSSIFQGSAVC
  VYSMNDVRRAFLGPFAHKEGPMHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMY
  NSVLPTGGRPLFLQVGANYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPSAEGLLLEE
  LHVFEDSAAVTSMQISSKRHQLYVASRSAVAQIALHRCAAHGRVCTECCLARDPYCAWDGVACTRFQPS
  AKRRFRRQDVRNGDPSTLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQV
  LAEERTERTARGLLLRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGP
  KLWYRDFLQLVEPGGGGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGPRSATHW
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290061
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001290061.1, NP_001276990.1
• RefSeq Size: 2830 bp
• RefSeq ORF: 2265 bp
• Locus ID: 7869
• Cytogenetics: 3p21.31
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance
• MW: 84.1 kDa
• Gene Summary: The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]