STAT5A (NM_001288720) Human Tagged ORF Clone

CAT#: RG239510

STAT5A (GFP-tagged) - Human signal transducer and activator of transcription 5A (STAT5A), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: STAT5A
• Synonyms: MGF; STAT5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239510 representing NM_001288720.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGGCTGGATCCAGGCCCAGCAGCTGCAGGGAGACGCGCTGCGCCAGATGCAGGTGCTGTACGGC
  CAGCACTTCCCCATCGAGGTCCGGCACTACTTGGCCCAGTGGATTGAGAGCCAGCCATGGGATGCCATT
  GACTTGGACAATCCCCAGGACAGAGCCCAAGCCACCCAGCTCCTGGAGGGCCTGGTGCAGGAGCTGCAG
  AAGAAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTACTGAAGATCAAGCTGGGGCACTACGCCACG
  CAGCTCCAGAAAACATATGACCGCTGCCCCCTGGAGCTGGTCCGCTGCATCCGGCACATTCTGTACAAT
  GAACAGAGGCTGGTCCGAGAAGCCAACAATTGCAGCTCTCCGGCTGGGATCCTGGTTGACGCCATGTCC
  CAGAAGCACCTTCAGATCAACCAGACATTTGAGGAGCTGCGACTGGTCACGCAGGACACAGAGAATGAG
  CTGAAGAAACTGCAGCAGACTCAGGAGTACTTCATCATCCAGTACCAGGAGAGCCTGAGGATCCAAGCT
  CAGTTTGCCCAGCTGGCCCAGCTGAGCCCCCAGGAGCGTCTGAGCCGGGAGACGGCCCTCCAGCAGAAG
  CAGGTGTCTCTGGAGGCCTGGTTGCAGCGTGAGGCACAGACACTGCAGCAGTACCGCGTGGAGCTGGCC
  GAGAAGCACCAGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGATGACGAGCTGATC
  CAGTGGAAGCGGCGGCAGCAGCTGGCCGGGAACGGCGGGCCCCCCGAGGGCAGCCTGGACGTGCTACAG
  TCCTGGTGTGAGAAGTTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACCTC
  TGCCAGCAGCTGCCCATCCCCGGCCCAGTGGAGGAGATGCTGGCCGAGGTCAACGCCACCATCACGGAC
  ATTATCTCAGCCCTGGTGACCAGCACATTCATCATTGAGAAGCAGCCTCCTCAGGTCCTGAAGACCCAG
  ACCAAGTTTGCAGCCACCGTACGCCTGCTGGTGGGCGGGAAGCTGAACGTGCACATGAATCCCCCCCAG
  GTGAAGGCCACCATCATCAGTGAGCAGCAGGCCAAGTCTCTGCTTAAAAATGAGAACACCCGCAACGAG
  TGCAGTGGTGAGATCCTGAACAACTGCTGCGTGATGGAGTACCACCAAGCCACGGGCACCCTCAGTGCC
  CACTTCAGGAACATGTCACTGAAGAGGATCAAGCGTGCTGACCGGCGGGGTGCAGAGTCCGTGACAGAG
  GAGAAGTTCACAGTCCTGTTTGAGTCTCAGTTCAGTGTTGGCAGCAATGAGCTTGTGTTCCAGGTGAAG
  GGCAGGGTGCCATTTGCCGTGCCTGACAAAGTGCTGTGGCCGCAGCTGTGTGAGGCGCTCAACATGAAA
  TTCAAGGCCGAAGTGCAGAGCAACCGGGGCCTGACCAAGGAGAACCTCGTGTTCCTGGCGCAGAAACTG
  TTCAACAACAGCAGCAGCCACCTGGAGGACTACAGTGGCCTGTCCGTGTCCTGGTCCCAGTTCAACAGG
  GAGAACTTGCCGGGCTGGAACTACACCTTCTGGCAGTGGTTTGACGGGGTGATGGAGGTGTTGAAGAAG
  CACCACAAGCCCCACTGGAATGATGGGGCCATCCTAGGTTTTGTGAATAAGCAACAGGCCCACGACCTG
  CTCATCAACAAGCCCGACGGGACCTTCTTGTTGCGCTTTAGTGACTCAGAAATCGGGGGCATCACCATC
  GCCTGGAAGTTTGACTCCCCGGAACGCAACCTGTGGAACCTGAAACCATTCACCACGCGGGATTTCTCC
  ATCAGGTCCCTGGCTGACCGGCTGGGGGACCTGAGCTATCTCATCTATGTGTTTCCTGACCGCCCCAAG
  GATGAGGTCTTCTCCAAGTACTACACTCCTGTGCTGGCTAAAGCTGTTGATGGATATGTGAAACCACAG
  ATCAAGCAAGTGGTCCCTGAGTTTGTGAATGCATCTGCAGATGCTGGGGGCAGCAGCGCCACGTACATG
  GACCAGGCCCCCTCCCCAGCTGTGTGCCCCCAGGCTCCCTATAACATGTACCCACAGAACCCTGACCAT
  GTACTCGATCAGGATGGAGAATTCGACCTGGATGAGACCATGGATGTGGCCAGGCACGTGGAGGAACTC
  TTACGCCGACCAATGGACAGTCTTGACTCCCGCCTCTCGCCCCCTGCCGGTCTTTTCACCTCTGCCAGA
  GGCTCCCTCTCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239510
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRAQATQLLEGLVQELQ
  KKAEHQVGEDGFLLKIKLGHYATQLQKTYDRCPLELVRCIRHILYNEQRLVREANNCSSPAGILVDAMS
  QKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLAQLSPQERLSRETALQQK
  QVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQ
  SWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQ
  TKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSA
  HFRNMSLKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKGRVPFAVPDKVLWPQLCEALNMK
  FKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGWNYTFWQWFDGVMEVLKK
  HHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSPERNLWNLKPFTTRDFS
  IRSLADRLGDLSYLIYVFPDRPKDEVFSKYYTPVLAKAVDGYVKPQIKQVVPEFVNASADAGGSSATYM
  DQAPSPAVCPQAPYNMYPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFTSAR
  GSLS
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288720
• ORF Size: 2289 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288720.1, NP_001275649.1
• RefSeq Size: 4132 bp
• RefSeq ORF: 2292 bp
• Locus ID: 6776
• Cytogenetics: 17q21.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Chronic myeloid leukemia, ErbB signaling pathway, Jak-STAT signaling pathway, Pathways in cancer
• MW: 87.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]