TLE3 (NM_001282979) Human Tagged ORF Clone

CAT#: RG239518

TLE3 (GFP-tagged) - Human transducin-like enhancer of split 3 (TLE3), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TLE3
• Synonyms: ESG; ESG3; GRG3; HsT18976
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239518 representing NM_001282979.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCT
  GAGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAG
  TACGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTAT
  GGCTTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATC
  ATGCCTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACC
  ATGACGGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACAC
  GGCCCCCCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGG
  AGCAGCTCCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAG
  AACCACCATGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGC
  CTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAG
  GAGAAGGACAGCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCC
  AATGAGGACCCCGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCC
  CGTAGCCTGAAAAAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCC
  AAGACCAAAGACCTTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCA
  AGGAACGACGCCCCAACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCG
  GGCATGGACCCGATAGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCGGCGCCCTTC
  GCCATGATGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGCCTCCACAAC
  ATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCAATGGTTGGT
  TTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAGGAAAA
  CCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGCCCTG
  GCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGTGCC
  GTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATC
  AGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTCC
  TGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGAC
  CTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCC
  ATTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCAC
  AACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATCTCCCATGAT
  GGCACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAG
  CTACAGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCT
  GTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAGCTGCACCTG
  CACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACTGGGAAAGAT
  AACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCCTCGTCTGTC
  TTGAGTTGTGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAGGCCACAGTT
  TATGAGGTCATCTAC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239518
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSY
  GLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATH
  GPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSES
  LRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKA
  RSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPP
  GMDPIASALRTPISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSPMVG
  FDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVCA
  VTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWD
  LASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHD
  GTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHL
  HESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATV
  YEVIY
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282979
• ORF Size: 2292 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282979.2
• RefSeq Size: 5736 bp
• RefSeq ORF: 2295 bp
• Locus ID: 7090
• Cytogenetics: 15q23
• Protein Families: Transcription Factors
• MW: 83.1 kDa
• Gene Summary: 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]'