TLE3 (NM_001282980) Human Tagged ORF Clone
CAT#: RG239528
- TrueORF®
TLE3 (GFP-tagged) - Human transducin-like enhancer of split 3 (TLE3), transcript variant 4
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | TLE3 |
Synonyms | ESG; ESG3; GRG3; HsT18976 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239528 representing NM_001282980.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTATCCGCAGGGCAGACATCCGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCT GAGTCTTGTGACAGGATCAAAGACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAG TACGACAAGCTGGCAAACGAGAAGACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTAT GGCTTGAACATTGAAATGCACAAGCAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATC ATGCCTTTCCTGTCACAAGAGCACCAGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACC ATGACGGAGCTGAACGCCATCATCGGGCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACAC GGCCCCCCGGTCCAGTTGCCACCCCACCCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGG AGCAGCTCCGGGCTGCTGGCACTGGGCGCCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAG AACCACCATGAACTCGATCACAGAGAGAGAGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGC CTCCGGGCCAGTGAGAAGCACCGGGGCTCTGCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAG GAGAAGGACAGCTTGAGCCGATACGACAGTGATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCC AATGAGGACCCCGCAACGCCCCGGGTCAGCCCGGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCC CGTAGCCTGAAAAAAGATGCCCCCACCAGCCCTGCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCC AAGACCAAAGACCTTGGTCATAACGACAAATCCTCCACCCCTGGGCTCAAGTCCAACACACCAACCCCA AGGAACGACGCCCCAACTCCAGGCACCAGCACGACCCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCG GGCATGGACCCGATAGGTATAATGGCCTCGGCTCTGCGCACGCCCATCTCCATCACCAGCTCCTATGCG GCGCCCTTCGCCATGATGAGCCACCATGAGATGAACGGCTCCCTCACCAGTCCTGGCGCCTACGCCGGC CTCCACAACATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGCCGCTGCCTATGGCCGATCGCCA ATGGTTGGTTTTGACCCTCACCCCCCGATGCGGGCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCT GGAGGAAAACCAGCGTACTCATTCCATGTGAGTGCTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCAC GACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGCCCGGCAGATCAACACACTCAGCCACGGGGAGGTG GTGTGTGCCGTGACCATCAGCAACCCCACGAGGCACGTCTACACAGGTGGCAAGGGCTGCGTGAAGATC TGGGACATCAGCCAGCCAGGCAGCAAGAGCCCCATCTCCCAGCTGGACTGCCTGAACAGGGACAATTAC ATCCGCTCCTGCAAGCTGCTCCCTGATGGGCGCACGCTCATCGTGGGCGGCGAGGCCAGCACGCTCACC ATCTGGGACCTGGCCTCGCCCACGCCCCGCATCAAGGCCGAGCTGACGTCCTCGGCTCCCGCCTGTTAT GCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCTGCTGCAGCGATGGGAACATTGCTGTCTGG GACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCACACAGATGGGGCCAGCTGCATAGACATC TCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAACACGGTGCGCTCCTGGGACCTGCGGGAG GGCCGACAGCTACAGCAGCATGACTTCACTTCCCAGATCTTCTCGCTGGGCTACTGCCCCACTGGGGAG TGGCTGGCTGTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCACCACACCAAGCCTGACAAGTACCAG CTGCACCTGCACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTACTGCGGCAAGTGGTTCGTGAGCACT GGGAAAGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCAGCATATTCCAGTCTAAAGAATCC TCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGTAACAGGCTCTGGTGACAAGAAG GCCACAGTTTATGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239528
Blue=ORF Red=Cloning site Green=Tag(s) MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSY GLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATH GPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSES LRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKA RSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPP GMDPIGIMASALRTPISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSP MVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEV VCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLT IWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDI SHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQ LHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKK ATVYEVIY TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282980 |
ORF Size | 2301 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001282980.2 |
RefSeq Size | 5745 bp |
RefSeq ORF | 2304 bp |
Locus ID | 7090 |
Cytogenetics | 15q23 |
Protein Families | Transcription Factors |
MW | 83.4 kDa |
Gene Summary | 'This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]' |
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