HIF1 beta (ARNT) (NM_001286035) Human Tagged ORF Clone

CAT#: RG239539

ARNT (GFP-tagged) - Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: ARNT
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239539 representing NM_001286035.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGT
  GGAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGATGATGATGGAGAAGGG
  AACAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGATAAGGAGCGGTTTGCCAGGTCGGAT
  GATGAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGA
  CGGAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCTGGCTCGA
  AAACCAGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAAC
  ACATCCACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAG
  GCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGAGACAGGCAGGGTGGTGTATGTGTCTGACTCCGTG
  ACTCCTGTTTTGAACCAGCCACAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGAT
  GATGTGGATAAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTA
  AAGACTGGAACAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCG
  TTTATTTGCCGAATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCTTTGTG
  AGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCACTTCGTGGTGGTCCACTGC
  ACAGGCTACATCAAGGCCTGGCCCCCAGCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGC
  CTAGTGGCCATTGGCAGATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAA
  CCAACAGAGTTCATCTCCCGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCT
  ACTGTTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCCTGAAGACCAG
  CAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAAATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGG
  TTCCGGTCTAAGAACCAAGAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTACTCA
  GATGAAATTGAGTACATCATCTGTACCAACACCAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACA
  CTCTCCAACACAATCCAGAGGCCACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGA
  CAGCTGGCACCCAGGCAGCAGCAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCC
  AGCTACAATCATTCCCAGGTGGTTCAGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCTTGAG
  AAGTCAGATGGTTTATTTGCCCAGGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCG
  GATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTATTCTCCCAGGGCAACACA
  TTCCCTCCTACCCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTC
  CAGCCATCAGCTTCTGCAGGACAGATGTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCA
  ACCCCAACTTGGACCCCTACTACCCGCTCAGGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCT
  AAGACTCGTACTTCCCAGTTTGGTGTGGGCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTC
  CCTGGTGCCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGTGGATCTAACTTT
  GCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGACACGGACAGCAGAGGGTGTGGGTGTCTGGCCA
  CAGTGGCAGGGCCAGCAGCCTCATCATCGTTCAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCA
  CAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGATGCTGTCCATGCTGGGAGATCAGAGCAACAGCTAC
  AACAATGAAGAATTCCCTGATCTAACTATGTTTCCCCCCTTTTCAGAA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239539
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSD
  DEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGN
  TSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPD
  DVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFV
  RNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPADDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQ
  PTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFR
  FRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSG
  QLAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEIYHNINA
  DQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSNPTQGA
  TPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNF
  APETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSY
  NNEEFPDLTMFPPFSE
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286035
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286035.2
• RefSeq Size: 4944 bp
• RefSeq ORF: 2328 bp
• Locus ID: 405
• Cytogenetics: 1q21.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• MW: 85.7 kDa
• Gene Summary: 'This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]'