HIF1 beta (ARNT) (NM_001286035) Human Tagged ORF Clone
CAT#: RG239539
- TrueORF®
ARNT (GFP-tagged) - Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 5
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | ARNT |
Synonyms | bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239539 representing NM_001286035.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGT GGAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGATGATGATGGAGAAGGG AACAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGATAAGGAGCGGTTTGCCAGGTCGGAT GATGAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGA CGGAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCTGGCTCGA AAACCAGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAAC ACATCCACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAG GCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGAGACAGGCAGGGTGGTGTATGTGTCTGACTCCGTG ACTCCTGTTTTGAACCAGCCACAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGAT GATGTGGATAAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTA AAGACTGGAACAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCG TTTATTTGCCGAATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCTTTGTG AGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCACTTCGTGGTGGTCCACTGC ACAGGCTACATCAAGGCCTGGCCCCCAGCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGC CTAGTGGCCATTGGCAGATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAA CCAACAGAGTTCATCTCCCGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCT ACTGTTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCCTGAAGACCAG CAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAAATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGG TTCCGGTCTAAGAACCAAGAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTACTCA GATGAAATTGAGTACATCATCTGTACCAACACCAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACA CTCTCCAACACAATCCAGAGGCCACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGA CAGCTGGCACCCAGGCAGCAGCAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCC AGCTACAATCATTCCCAGGTGGTTCAGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCTTGAG AAGTCAGATGGTTTATTTGCCCAGGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCG GATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACAGCTATTCTCCCAGGGCAACACA TTCCCTCCTACCCCCCGGCCGGCAGAGAATTTCAGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTC CAGCCATCAGCTTCTGCAGGACAGATGTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCA ACCCCAACTTGGACCCCTACTACCCGCTCAGGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCT AAGACTCGTACTTCCCAGTTTGGTGTGGGCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTC CCTGGTGCCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGTGGATCTAACTTT GCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGACACGGACAGCAGAGGGTGTGGGTGTCTGGCCA CAGTGGCAGGGCCAGCAGCCTCATCATCGTTCAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCA CAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGATGCTGTCCATGCTGGGAGATCAGAGCAACAGCTAC AACAATGAAGAATTCCCTGATCTAACTATGTTTCCCCCCTTTTCAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239539
Blue=ORF Red=Cloning site Green=Tag(s) MTSDVPSLGPAIASGNSGPGIQGGGAIVQRAIKRRPGLDFDDDGEGNSKFLRCDDDQMSNDKERFARSD DEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGN TSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPD DVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGSSSVDPVSVNRLSFV RNRCRNGLGSVKDGEPHFVVVHCTGYIKAWPPADDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNVCQ PTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFR FRSKNQEWLWMRTSSFTFQNPYSDEIEYIICTNTNVKNSSQEPRPTLSNTIQRPQLGPTANLPLEMGSG QLAPRQQQQQTELDMVPGRDGLASYNHSQVVQPVTTTGPEHSKPLEKSDGLFAQDRDPRFSEIYHNINA DQSKGISSSTVPATQQLFSQGNTFPPTPRPAENFRNSGLAPPVTIVQPSASAGQMLAQISRHSNPTQGA TPTWTPTTRSGFSAQQVATQATAKTRTSQFGVGSFQTPSSFSSMSLPGAPTASPGAAAYPSLTNRGSNF APETGQTAGQFQTRTAEGVGVWPQWQGQQPHHRSSSSEQHVQQPPAQQPGQPEVFQEMLSMLGDQSNSY NNEEFPDLTMFPPFSE TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286035 |
ORF Size | 2325 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001286035.2 |
RefSeq Size | 4944 bp |
RefSeq ORF | 2328 bp |
Locus ID | 405 |
Cytogenetics | 1q21.3 |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Pathways in cancer, Renal cell carcinoma |
MW | 85.7 kDa |
Gene Summary | 'This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]' |
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