Epac2 (RAPGEF4) (NM_001282901) Human Tagged ORF Clone

CAT#: RG239570

RAPGEF4 (GFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: RAPGEF4
• Synonyms: CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239570 representing NM_001282901.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATAAGAGATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGGGAACTGAACTGGTGGAC
  TGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCATGTGGCAAGTCCTGTTA
  GAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATATTTATTCTATCGATTT
  CTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGTGTGATGAGGAGCTC
  CAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGATCCTTCGCAAACCA
  CCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATTAAAGCCTTATCC
  CATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGG
  ACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATCAGTGAATGTA
  GTCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTAGCACTAGTG
  AATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAGTAGACAAG
  GAGGATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGACCAAGAT
  GTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCTCAGCAA
  AAGTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCCTTGAG
  GCAACTTTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTATGCCA
  AATACCCAGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAGGAG
  AAAATGGATTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGTAT
  GGAGACCTCCTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGAT
  GATGCCCGGATGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCA
  GAAGATGCAAAGGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGA
  GCCCAGAAGCGCCAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACC
  TACACAACCATTCGGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTG
  GGCTCCGGGGAGGGCCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAAT
  GATGTTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGAT
  TCACTGACTCCCTTACCAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGC
  TCCAAAGATTTAGCATACCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAG
  CTAATCTATCACACATTTGGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGG
  AGATTTAATGAAATTCAGTTTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTT
  CAGCTATTAAAAAAATTTATTAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTT
  GCCATCGTCATGGGACTAAGTAACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAGC
  AAGTTCAAGAAGTTCTATGCGGAGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGG
  CTGACAGTAGCTAAGCTGGAACCTCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTT
  ACTCATGAGGGGAACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAAT
  ACGGCCAGAACAGTGAGATACTACAGGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAAC
  CATCAGGATGTCCGGAGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATG
  TCACACAGATTAGAGCCTCGTCGACCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239570
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRF
  LDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALS
  HLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALV
  NDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQ
  KYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQE
  KMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQIS
  EDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKL
  GSGEGLIIVKMSSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMS
  SKDLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRV
  QLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYR
  LTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKN
  HQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282901
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282901.2
• RefSeq Size: 3725 bp
• RefSeq ORF: 2376 bp
• Locus ID: 11069
• Cytogenetics: 2q31.1
• Protein Pathways: Leukocyte transendothelial migration
• MW: 91.4 kDa