MRTFA (NM_001282662) Human Tagged ORF Clone

CAT#: RG239590

MKL1 (GFP-tagged) - Human megakaryoblastic leukemia (translocation) 1 (MKL1), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: MRTFA
• Synonyms: BSAC; MAL; MKL; MKL1; MRTF-A
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239590 representing NM_001282662.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCGCCTTTGAAAAGTCCAGCCGCATTTCATGAGCAGAGAAGGAGCTTGGAGCGGGCCAGGACAGAG
  GACTATCTCAAACGGAAGATTCGTTCCCGGCCGGAGAGATCGGAGCTGGTCAGGATGCACATTTTGGAA
  GAGACCTCGGCTGAGCCATCCCTCCAGGCCAAGCAGCTGAAGCTGAAGAGAGCCAGACTAGCCGATGAC
  CTCAATGAGAAGATTGCACAGAGGCCTGGCCCCATGGAGCTGGTGGAGAAGAACATCCTTCCTGTTGAG
  TCCAGCCTGAAGGAAGCCATCATTGTGGGCCAGGTGAACTATCCCAAAGTAGCAGACAGCTCTTCCTTC
  GATGAGGACAGCAGCGATGCCTTATCCCCCGAGCAGCCTGCCAGCCATGAGTCCCAGGGTTCTGTGCCG
  TCACCCCTGGAGGCCCGAGTCAGCGAACCACTGCTCAGTGCCACCTCTGCATCCCCCACCCAGGTTGTG
  TCTCAACTTCCGATGGGCCGGGATTCCAGAGAAATGCTTTTCCTGGCAGAGCAGCCTCCTCTGCCTCCC
  CCACCTCTGCTGCCTCCCAGCCTCACCAATGGAACCACTATCCCCACTGCCAAGTCCACCCCCACACTC
  ATTAAGCAAAGCCAACCCAAGTCTGCCAGTGAGAAGTCACAGCGCAGCAAGAAGGCCAAGGAGCTGAAG
  CCAAAGGTGAAGAAGCTCAAGTACCACCAGTACATCCCCCCGGACCAGAAGCAGGACAGGGGGGCACCC
  CCCATGGACTCATCCTACGCCAAGATCCTGCAGCAGCAGCAGCTCTTCCTCCAGCTGCAGATCCTCAAC
  CAGCAGCAGCAGCAGCACCACAACTACCAGGCCATCCTGCCTGCCCCGCCAAAGTCAGCAGGCGAGGCC
  CTGGGAAGCAGCGGGACCCCCCCAGTACGCAGCCTCTCCACTACCAATAGCAGCTCCAGCTCGGGCGCC
  CCTGGGCCCTGTGGGCTGGCACGTCAGAACAGCACCTCACTGACTGGCAAGCCGGGAGCCCTGCCGGCC
  AACCTGGACGACATGAAGGTGGCAGAGCTGAAGCAGGAGCTGAAGTTGCGATCACTGCCTGTCTCGGGC
  ACCAAAACTGAGCTGATTGAGCGCCTTCGAGCCTATCAAGACCAAATCAGCCCTGTGCCAGGAGCCCCC
  AAGGCCCCTGCCGCCACCTCTATCCTGCACAAGGCTGGCGAGGTGGTGGTAGCCTTCCCAGCGGCCCGG
  CTGAGCACGGGGCCAGCCCTGGTGGCAGCAGGCCTGGCTCCAGCTGAGGTGGTGGTGGCCACGGTGGCC
  AGCAGTGGGGTGGTGAAGTTTGGCAGCACGGGCTCCACGCCCCCCGTGTCTCCCACCCCCTCGGAGCGC
  TCACTGCTCAGCACGGGCGATGAAAACTCCACCCCCGGGGACACCTTTGGTGAGATGGTGACATCACCT
  CTGACGCAGCTGACCCTGCAGGCCTCGCCACTGCAGATCCTCGTGAAGGAGGAGGGCCCCCGGGCCGGG
  TCCTGTTGCCTGAGCCCTGGGGGGCGGGCGGAGCTAGAGGGGCGCGACAAGGACCAGATGCTGCAGGAG
  AAAGACAAGCAGATCGAGGCGCTGACGCGCATGCTCCGGCAGAAGCAGCAGCTGGTGGAGCGGCTCAAG
  CTGCAGCTGGAGCAGGAGAAGCGAGCCCAGCAGCCCGCCCCCGCCCCCGCCCCCCTCGGCACCCCCGTG
  AAGCAGGAGAACAGCTTCTCCAGCTGCCAGCTGAGCCAGCAGCCCCTGGGCCCCGCTCACCCATTCAAC
  CCCAGCCTGGCGGCCCCAGCCACCAACCACATAGACCCTTGTGCTGTGGCCCCGGGGCCCCCGTCCGTG
  GTGGTGAAGCAGGAAGCCTTGCAGCCTGAGCCCGAGCCGGTCCCCGCCCCCCAGTTGCTTCTGGGGCCT
  CAGGGCCCCAGCCTCATCAAGGGGGTTGCACCTCCCACCCTCATCACCGACTCCACAGGGACCCACCTT
  GTCCTCACCGTGACCAATAAGAATGCAGACAGCCCTGGCCTGTCCAGTGGGAGCCCCCAGCAGCCCTCG
  TCCCAGCCTGGCTCTCCAGCGCCTGCCCCCTCTGCCCAGATGGACCTGGAGCACCCACTGCAGCCCCTC
  TTTGGGACCCCCACTTCTCTGCTGAAGAAGGAACCACCTGGCTATGAGGAAGCCATGAGCCAGCAGCCC
  AAACAGCAGGAAAATGGTTCCTCAAGCCAGCAGATGGACGACCTGTTTGACATTCTCATTCAGAGCGGA
  GGTAACCCCAGAAATTTCAGCAGATTTCAAGGAGCCGCCATCCCTGCCAGGGAAGGAGAAGCCATCCCC
  GAAGACAGTCTGTGGGTCCCCCCTGGCAGCACAGCCATCACCTTCTGC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239590
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADD
  LNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVP
  SPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTL
  IKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILN
  QQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPA
  NLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAAR
  LSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSP
  LTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLK
  LQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSV
  VVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPS
  SQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSG
  GNPRNFSRFQGAAIPAREGEAIPEDSLWVPPGSTAITFC
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282662
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001282662.1, NP_001269591.1
• RefSeq Size: 4560 bp
• RefSeq ORF: 2697 bp
• Locus ID: 57591
• Cytogenetics: 22q13.1-q13.2
• Protein Families: Transcription Factors
• MW: 85.5 kDa
• Gene Summary: The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]