SMARCA6 (HELLS) (NM_001289069) Human Tagged ORF Clone

CAT#: RG239603

HELLS (GFP-tagged) - Human helicase, lymphoid-specific (HELLS), transcript variant 4

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HELLS
• Synonyms: ICF4; LSH; Nbla10143; PASG; SMARCA6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239603 representing NM_001289069.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGCGGAACGGCCCGCGGGCAGCGGCGGCTCGGAGGCTCCAGCAATGGTTGAACAACTGGACACT
  GCTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGG
  AAGATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCGACAGAAATTCGGTACCGTAGACTTCAA
  CATTTGCTTGAAAAAAGCAATATATACTCCAAATTTTTATTGACGAAAATGGAACAGCAACAATTAGAG
  GAACAGAAGAAGAAAGAAAAATTGGAGAGAAAAAAGGAGTCTTTAAAAGTTAAAAAGGGTAAAAATTCA
  ATTGATGCAAGTGAAGAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGATGAATCATACAATATT
  TCAGAGGTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAGGAGAATGAGGATGAA
  AACTCCTCCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGAATAGTATAATTAAA
  GATAGATTGTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGACCCAGTCCGGAAGTGTAATGGT
  CAGCCAGTACCTTTTCAACAACCAAAGCACTTCACTGGAGGAGTGATGCGATGGTACCAAGTAGAAGGC
  ATGGAATGGCTTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGGGATTGGGT
  AAGACAGTTCAGTGCATTGCTACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACCTTTTCTTGTC
  TGTGGCCCTTTGTCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGATATCCCTACA
  ATGTTATATCATGGAACCCAGGAGGAACGTCAAAAATTGCATTGCTATTGGAAATACTTAATAGTAGAT
  GAAGGACACAGGATTAAGAATATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCAATGCTGATAAC
  AAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCTAAACTTTTTG
  TTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTTTCTGAAACT
  GCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTATTGCATATGCTGCACCAGATTTTAACACCT
  TTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTTCCTCCTAAACGAGAAGTAGTCGTTTAT
  GCTCCACTTTCAAAGAAGCAGGAGATCTTTTATACAGCCATTGTGAACCGTACAATTGCAAACATGTTT
  GGATCCAGTGAGAAAGAAACAATTGAGTTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAAAATCA
  ATAAATTACAGCAAAATAGATGATTTCCCTAATGAATTGGAAAAACTGATCAGTCAAATACAGCCAGAG
  GTGGACCGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTGCAG
  AATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATATTTGATTGAATATCCTATAGACCCTGTT
  ACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCTGGGAAGTTCTTGATTTTGGATCGAATG
  CTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTTTTTTCACAAATGACAAGCATGTTGGACATT
  TTGATGGATTACTGCCATCTCAGAGATTTCAACTTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAG
  AGAGAAAAAAACATGCACAGCTTCAACACGGATCCAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCT
  GGTGGCCTGGGCATTAATCTGACTGCAGCAGATACAGTTATCATTTATGATAGTGATTGGAACCCCCAG
  TCGGATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAGACAAAGCCAGTTGTTGTTTATCGCCTT
  GTTACAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGGAAACTGGAAAAGTTG
  ATCATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTAAATCTGTCTAAGAATTTCTTAGATCCT
  AAGGAATTAATGGAATTATTAAAATCTAGAGATTATGAAAGGGAAATAAAAGGATCAAGAGAGAAGGTC
  ATTAGTGATAAAGATCTAGAGTTGTTGTTAGATCGAAGTGATCTTATTGATCAAATGAATGCTTCAGGA
  CCAATTAAAGAGAAGATGGGGATATTCAAGATATTAGAAAATTCTGAAGATTCCAGTCCTGAATGTTTG
  TTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239603
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQ
  HLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNI
  SEVMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNG
  QPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLV
  CGPLSTLPNWMAEFKRFTPDIPTMLYHGTQEERQKLHCYWKYLIVDEGHRIKNMKCRLIRELKRFNADN
  KLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESWFDITSLSETAEDIIAKEREQNVLHMLHQILTP
  FLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIANMFGSSEKETIELSPTGRPKRRTRKS
  INYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPYLIEYPIDPV
  TQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSRLDGSMSYSE
  REKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQTKPVVVYRL
  VTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYEREIKGSREKV
  ISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289069
• ORF Size: 2418 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289069.2
• RefSeq Size: 3067 bp
• RefSeq ORF: 2421 bp
• Locus ID: 3070
• Cytogenetics: 10q23.33
• MW: 93.8 kDa
• Gene Summary: 'This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]'