LONP2 (NM_001300948) Human Tagged ORF Clone

CAT#: RG239606

LONP2 (GFP-tagged) - Human lon peptidase 2, peroxisomal (LONP2), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: LONP2
• Synonyms: LONP; LONPL; PLON; PSLON
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239606 representing NM_001300948.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCATCAGTGAGCCCCATCCAGATCCCCAGTCGCCTCCCGCTGCTGCTCACCCACGAGGGCGTCCTG
  CTGCCCGGCTCCACCATGCGCACCAGCGTGGACTCGGCCCGCAACCTGCAGCTGGTGCGGAGCCGCCTT
  CTGAAGGGCACGTCGCTGCAAAGCACCATCCTGGGCGTCATCCCCAACACGCCTGACCCCGCCAGCGAC
  GCGCAGGACCTGCCGCCGCTGCACAGGATTGGCACAGCTGCACTGGCCGTTCAGGTTGTGGGCAGTAAC
  TGGCCCAAGCCCCACTACACTCTGTTGATTACAGGCCTATGCCGTTTCCAGATTGTACAGGTCTTAAAA
  GAGAAGCCATATCCCATTGCTGAAGTGGAGCAGTTGGACCGACTTGAGGAGTTTCCCAACACCTGTAAA
  ATGAGGGAGGAGCTAGGAGAACTATCAGAGCAGTTTTACAAATATGCAGTACAAATTTTAGATGCTGTG
  AGCCTAGAGGAGCGGTTCAAGATGACTATACCACTGCTTGTCAGACAAATTGAAGGCCTGAAATTGCTT
  CAAAAAACCAGAAAACCCAAGCAAGATGATGATAAGAGGGTTATAGCAATACGCCCTATTAGGAGAATT
  ACACATATCTCAGGTACTTTAGAAGATGAAGATGAAGATGAAGATAATGATGACATTGTCATGCTAGAG
  AAAAAAATACGAACATCTAGTATGCCAGAGCAGGCCCATAAAGTCTGTGTCAAAGAGATAAAGAGACTC
  AAAAAAATGCCTCAGTCAATGCCAGAATATGCTCTGACTAGAAATTATTTGGAACTTATGGTAGAACTT
  CCTTGGAACAAAAGTACAACTGACCGCCTGGACATTAGGGCAGCCCGGATTCTTCTGGATAATGACCAT
  TACGCCATGGAAAAATTGAAGAAAAGAGTACTGGAATACTTGGCTGTCAGACAGCTCAAAAATAACCTG
  AAGGGCCCAATCCTATGCTTTGTTGGCCCTCCTGGAGTTGGTAAAACAAGTGTGGGAAGATCAGTGGCC
  AAGACTCTAGGTCGAGAGTTCCACAGGATTGCACTTGGAGGAGTATGTGATCAGTCTGACATTCGAGGA
  CACAGGCGCACCTATGTTGGCAGCATGCCTGGTCGCATCATCAACGGCTTGAAGACTGTGGGAGTGAAC
  AACCCAGTGTTCCTATTAGATGAGGTTGACAAACTGGGAAAAAGTCTACAGGGTGATCCAGCAGCAGCT
  CTGCTTGAGGTGTTGGATCCTGAACAAAACCATAACTTCACAGATCATTATCTAAATGTGGCCTTTGAC
  CTTTCTCAAGTTCTTTTTATAGCTACTGCCAACACCACTGCTACCATTCCAGCTGCCTTGTTGGACAGA
  ATGGAGATCATTCAGGTTCCAGGTTATACACAGGAGGAGAAGATAGAGATTGCCCATAGGCACTTGATC
  CCCAAGCAGCTGGAACAACATGGGCTGACTCCACAGCAGATTCAGATACCCCAGGTCACCACTCTTGAC
  ATCATCACCAGGTATACCAGAGAGGCAGGGGTTCGTTCTCTGGATAGAAAACTTGGGGCCATTTGCCGA
  GCTGTGGCCGTGAAGGTGGCAGAAGGACAGCATAAGGAAGCCAAGTTGGACCGTTCTGATGTGACTGAG
  AGAGAAGGTTGCAGAGAACACATCTTAGAAGATGAAAAACCTGAATCTATCAGTGACACTACTGACTTG
  GCTCTACCACCTGAAATGCCGATTTTGATTGATTTCCATGCTCTGAAAGACATCCTTGGGCCCCCGATG
  TATGAAATGGAGGTATCTCAGCGTTTGAGTCAGCCAGGAGTAGCAATAGGTTTGGCTTGGACTCCCTTA
  GGTGGAGAAATCATGTTCGTGGAGGCGAGTCGAATGGATGGCGAGGGCCAGTTAACTCTGACCGGCCAG
  CTCGGGGACGTGATGAAGGAGTCCGCCCACCTCGCTATCAGCTGGCTCCGCAGCAACGCAAAGAAGTAC
  CAGCTGACCAATGCTTTTGGAAGTTTTGATCTTCTTGACAACACAGACATCCATCTGCACTTCCCAGCT
  GGAGCTGTCACAAAAGATGGACCATCTGCTGGAGTTACCATAGTAACCTGTCTCGCCTCACTTTTTAGT
  GGGCGGCTGGTACGTTCAGATGTAGCCATGACTGGAGAAATTACACTGAGAGGTCTTGTTCTTCCAGTG
  GGTGGAATTAAAGACAAAGTGCTGGCGGCACACAGAGCGGGACTGAAGCAAGTCATTATTCCTCGGAGA
  AATGAAAAAGACCTTGAGGGAATCCCAGGCAACGTACGACAGGATTTAAGTTTTGTCACAGCAAGCTGC
  CTGGATGAGGTTCTTAATGCAGCTTTTGATGGTGGCTTTACTGTCAAGACCAGACCTGGTCTGTTAAAT
  AGCAAACTG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239606
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSSVSPIQIPSRLPLLLTHEGVLLPGSTMRTSVDSARNLQLVRSRLLKGTSLQSTILGVIPNTPDPASD
  AQDLPPLHRIGTAALAVQVVGSNWPKPHYTLLITGLCRFQIVQVLKEKPYPIAEVEQLDRLEEFPNTCK
  MREELGELSEQFYKYAVQILDAVSLEERFKMTIPLLVRQIEGLKLLQKTRKPKQDDDKRVIAIRPIRRI
  THISGTLEDEDEDEDNDDIVMLEKKIRTSSMPEQAHKVCVKEIKRLKKMPQSMPEYALTRNYLELMVEL
  PWNKSTTDRLDIRAARILLDNDHYAMEKLKKRVLEYLAVRQLKNNLKGPILCFVGPPGVGKTSVGRSVA
  KTLGREFHRIALGGVCDQSDIRGHRRTYVGSMPGRIINGLKTVGVNNPVFLLDEVDKLGKSLQGDPAAA
  LLEVLDPEQNHNFTDHYLNVAFDLSQVLFIATANTTATIPAALLDRMEIIQVPGYTQEEKIEIAHRHLI
  PKQLEQHGLTPQQIQIPQVTTLDIITRYTREAGVRSLDRKLGAICRAVAVKVAEGQHKEAKLDRSDVTE
  REGCREHILEDEKPESISDTTDLALPPEMPILIDFHALKDILGPPMYEMEVSQRLSQPGVAIGLAWTPL
  GGEIMFVEASRMDGEGQLTLTGQLGDVMKESAHLAISWLRSNAKKYQLTNAFGSFDLLDNTDIHLHFPA
  GAVTKDGPSAGVTIVTCLASLFSGRLVRSDVAMTGEITLRGLVLPVGGIKDKVLAAHRAGLKQVIIPRR
  NEKDLEGIPGNVRQDLSFVTASCLDEVLNAAFDGGFTVKTRPGLLNSKL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001300948
• ORF Size: 2424 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001300948.3
• RefSeq Size: 4826 bp
• RefSeq ORF: 2427 bp
• Locus ID: 83752
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome, Protease
• MW: 90.1 kDa
• Gene Summary: In human, peroxisomes function primarily to catalyze fatty acid beta-oxidation and, as a by-product, produce hydrogen peroxide and superoxide. The protein encoded by this gene is an ATP-dependent protease that likely plays a role in maintaining overall peroxisome homeostasis as well as proteolytically degrading peroxisomal proteins damaged by oxidation. The protein has an N-terminal Lon N substrate recognition domain, an ATPase domain, a proteolytic domain, and, in some isoforms, a C-terminal peroxisome targeting sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]