CDC27 (NM_001293089) Human Tagged ORF Clone

CAT#: RG239636

CDC27 (GFP-tagged) - Human cell division cycle 27 (CDC27), transcript variant 3

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CDC27
• Synonyms: ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239636 representing NM_001293089.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACGGTGCTGCAGGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGAT
  GCGGTTTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGCAACC
  TGTTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACTACACCG
  CAATGCAAATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTA
  TCTGGTGGAGTGTTTAATAAGCAGAAAAGCCATGATGATATTGTTACTGAGTTTGGTGATTCAGCTTGC
  TTTACTCTTTCATTGTTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTAC
  CAAAAGAGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAAAAG
  CCAGATCCTGACCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGC
  ACAACACAAGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAACACCCCAG
  GACACAATTGAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCC
  TCAGTGTCTTATATTGATTCAGCTGTAATTTCACCTGATACTGTCCCACTGGGAACAGGAACTTCCATA
  TTATCTAAACAGGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGT
  CCATTAACCCCAAGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAA
  AACTACACTAATACACCTCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGTCTGTTGCC
  AGAATCGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGCCGAGAGGTAACTCCAATT
  CTTGCACAAACACAAAGTTCTGGTCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATTACA
  TCTCCCCCAAACGCACTGCCTCGAAGAAGTTCACGACTCTTTACTAGTGACAGCTCCACAACCAAGGAG
  AATAGCAAAAAATTAAAAATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAAT
  AAAGGAGGAATAACTCAACCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATT
  TCAGAAGGGAAAATATCCACAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGCAGGT
  TTGATGAGCCTTCTTCGTGAAATGGGGAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCT
  ATAAATATTTTGAGCCATCTACCTTCTCACCACTACAATACTGGTTGGGTACTGTGCCAAATTGGAAGG
  GCCTATTTTGAACTTTCAGAGTACATGCAAGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAAT
  TATAGAGTTGAAGGCATGGAGATCTACTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCA
  GTTCTGTCAAAAGACTTAACAGACATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGT
  TTCAGTCTGCAACGGGAACATGATATTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAAT
  TACGCTTATGCCTATACTCTATTAGGGCATGAGTTTGTCTTAACTGAAGAATTGGACAAAGCATTAGCT
  TGTTTTCGAAATGCTATCAGAGTCAATCCTAGACATTATAATGCATGGTATGGTTTAGGAATGATTTAT
  TACAAGCAAGAAAAATTCAGCCTTGCAGAAATGCATTTCCAAAAAGCGCTTGATATCAACCCTCAAAGT
  TCAGTTTTACTTTGCCACATTGGAGTAGTTCAACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACC
  CTAAACAAAGCCATTGTCATTGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTT
  GCAAATGAAAAATATAAGTCTGCTTTACAAGAACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCC
  CTCGTTTACTTCTTAATAGGAAAGGTTTACAAGAAGTTAGGTCAAACGCACCTCGCCCTGATGAATTTC
  TCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAACCAGATTAAAGAGGCAATTGATAAGCGTTAT
  CTTCCAGATGATGAGGAGCCAATAACCCAAGAAGAACAGATCATGGGAACAGATGAATCCCAGGAGAGC
  AGCATGACAGATGCGGATGACACACAACTTCATGCAGCTGAAAGTGATGAATTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239636
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTP
  QCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECY
  QKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQ
  DTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALS
  PLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPI
  LAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTN
  KGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEA
  INILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALS
  VLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALA
  CFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDT
  LNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNF
  SWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293089
• ORF Size: 2469 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001293089.3
• RefSeq Size: 5850 bp
• RefSeq ORF: 2472 bp
• Locus ID: 996
• Cytogenetics: 17q21.32
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 92.2 kDa
• Gene Summary: 'The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]'