CRNKL1 (NM_001278625) Human Tagged ORF Clone

CAT#: RG239653

CRNKL1 (GFP-tagged) - Human crooked neck pre-mRNA splicing factor 1 (CRNKL1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239653 representing NM_001278625.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGACAAAAACAGG
  CGCCACGGCTCGACAGAGCCACTCGTCCTCGCCTGGTCATCCCAGTTCCGGAGGCTGACCTGGGGATGC
  GCCCTGGACGCCCTGCACCGCAGTCCTTGCGTCGCTGCTAGTCAGCACGGCGTTACCCACCTCATCCGC
  AGCTCCAGGACGCCGCACTCCACGCGCTGCCGCAAGGAGGACGCGCAGCCTGGTCACCATGGAAACGGT
  GCCGCCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTCAGTCCTGCAAGTTCCGCTTCCGGTTCCGAGA
  TCCTGTTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGCTCTCAGAGCCGATTTCTAGCGTCCGTGCCG
  GGGACAGGTGTCCAGAGGTCGACTGCTGCAGACATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATT
  CCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCGGCTGAGGTACAGATAACTGCTGAACAACTCTTAAGA
  GAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCACCTCCACCTCAACAGAAGATCACAGATGAAGAAGAA
  TTAAATGATTATAAACTAAGGAAAAGGAAGACTTTTGAAGATAATATAAGAAAAAACAGGACTGTGATT
  AGTAACTGGATAAAATACGCACAATGGGAAGAAAGCCTAAAGGAGATTCAAAGGGCTCGATCCATATAC
  GAGCGTGCTTTAGATGTAGACTACCGAAATATTACACTCTGGCTGAAATACGCAGAAATGGAAATGAAG
  AATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAACAACGCTGCCTCGAGTTAATCAG
  TTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAG
  CGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTGAGATACAAA
  GAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATC
  AAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAGAGAGCTGTG
  GAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCCAAGTTTGAAGAAAATCAG
  AAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTTCAAAACAAGATGCCCAA
  GAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGGTATTGAAGATATCATT
  GTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGGTTT
  GATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATT
  GCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTATGCA
  CTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCCTCTTTGGAACTA
  ATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAAATACGACAGAAG
  AATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAACAAATTATTTAAA
  GTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTG
  GAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATT
  GACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGG
  AAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGGCGGTTG
  CTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAAAAGAA
  GGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACTGTGAAGAAAAG
  GAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGCTTCAGATAAG
  GAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCT
  GATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAA
  CTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGAT
  GAGGACGTCGATGAGAGTGAATCC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239653
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTATVENLTFQKDTLGNAVDKNRRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIR
  SSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVP
  GTGVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEE
  LNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMK
  NRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYK
  EVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQ
  KEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWF
  DYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLEL
  IPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFL
  EFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRL
  LQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDK
  ERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
  EDVDESES
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278625
• ORF Size: 2508 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001278625.1, NP_001265554.1
• RefSeq Size: 4377 bp
• RefSeq ORF: 2511 bp
• Locus ID: 51340
• Cytogenetics: 20p11.23
• Protein Pathways: Spliceosome
• MW: 99.6 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]