AASDH (NM_001286671) Human Tagged ORF Clone

CAT#: RG239693

AASDH (GFP-tagged) - Human aminoadipate-semialdehyde dehydrogenase (AASDH), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AASDH
• Synonyms: ACSF4; LYS2; NRPS998; NRPS1098
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239693 representing NM_001286671.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTCTTCAGGAATTGGTGCATAAGGCTGCCTCCTGTTATATGGACAGAGTAGCTGTATGTTTTGAT
  GAATGCAACAACCAGCTTCCAGTTTACTACACCTACAAGACTGTGGTTAATGCTGCTTCTGAATTATCA
  AATTTTCTGCTGTTACACTGTGACTTTCAAGGAATTCGGGAAATTGGTCTCTACTGCCAACCTGGGATA
  GACTTACCCTCTTGGATTTTAGGAATTCTCCAAGTCCCGGCTGCTTATGTACCTATCGAGCCAGATTCA
  CCACCGTCATTATCAACTCATTTTATGAAAAAATGTAATCTAAAGTATATCCTTGTTGAAAAAAAACAA
  ATTAATAAATTTAAATCTTTTCATGAAACATTATTGAACTATGATACATTTACAGTGGAACATAATGAC
  CTAGTGCTCTTCAGACTTCACTGGAAAAATACTGAGGTGAACTTGATGCTAAATGATGGAAAAGAGAAA
  TATGAAAAAGAAAAAATAAAAAGCATAAGTTCTGAGCATGTCAATGAAGAAAAAGCAGAAGAACACATG
  GATCTGAGGCTAAAGCATTGCTTAGCCTATGTTCTACATACATCAGGGACTACAGGGATACCGAAGATT
  GTCAGAGTGCCTCATAAGTGTATAGTACCAAATATCCAGCATTTTCGGGTACTTTTTGACATCACACAA
  GAAGATGTTTTGTTTCTGGCTTCACCTCTGACCTTCGATCCTTCTGTTGTGGAAATATTTCTTGCTCTA
  TCAAGTGGTGCCTCTCTGCTTATTGTACCAACTTCCGTCAAGTTGCTCCCATCAAAATTAGCCAGCGTT
  CTCTTTTCCCATCATAGAGTGACTGTTTTGCAGGCAACACCAACATTGCTTAGAAGATTTGGATCTCAG
  CTTATCAAGTCAACTGTTTTGTCAGCCACTACTTCTCTTCGAGTATTAGCCCTTGGTGGTGAAGCGTTT
  CCATCATTGACAGTTCTCAGAAGCTGGAGAGGAGAAGGCAATAAAACACAAATATTTAATGTTTATGGT
  ATCACAGAGGTATCAAGTTGGGCGACCATTTATAGGATTCCAGAGAAGACTCTTAACTCTACTCTCAAA
  TGTGAATTGCCTGTACAACTGGGATTTCCACTTCTTGGAACAGTAGTTGAAGTCAGAGATACTAATGGC
  TTCACAATTCAGGAAGGCAGTGGCCAAGTATTTTTAGGTGGCAGAAACAGAGTGTGTTTTCTTGATGAT
  GAAGTGACAGTACCACTTGGCACAATGCGAGCTACAGGAGACTTTGTGACTGTGAAAGATGGAGAGATT
  TTTTTTTTGGGACGAAAAGACAGTCAGATCAAACGTCATGGCAAACGTCTTAACATTGAACTTGTGCAA
  CAGGTTGCTGAAGAGCTTCAGCAAGTGGAGTCTTGTGCAGTTACATGGTATAATCAGGAAAAATTAATT
  CTCTTCATGGTGTCTAAAGATGCTTCAGTAAAAGAATACATCTTTAAAGAACTGCAGAAATATCTTCCA
  AGTCATGCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTACCATTTACATCCCACGGCAAAATTGAT
  GTTTCTGAGTTAAACAAGATATATTTAAACTACATAAACTTGAAGTCTGAGAATAAGCTCAGTGGGAAA
  GAGGACCTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCTGAATCTCCCAGAAGATCTTTTGAGG
  GTTCCTGATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTAAAGTCCATCCGGCTCCTCAGTGAG
  ATTGAAAAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATTATTCTCAGCAGTTCCATTTTAGAG
  ATTTATAATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTGACATTCAGGAAGAGTTGTGCCACA
  AAAAGGAAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACATCTTTACATCAGAAAGCCATCATG
  ACTTTCACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGCAGAGGGAGTCAAATTTTGTCTCTG
  AATTCCACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCCTTCTGACTCAGTTTCACAG
  ACCAACATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAGTCAAAAGATCCATCCTGT
  GTTGCAAAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAGAAAATGGAGTTACATGTGAGGTGG
  AGGTCAGACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTAATACCCACTTTTGATAAGTCATCT
  ACAACTGTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTTGACTTTTACTCTGGGAAGGTAAAA
  TGGGAACAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTATCTAAGTGTGGAAACTTTATTGTG
  GTGGGTTTGGCAGTTCTCTACCAGTGGACCAATCTTTTCATCCCCGTGTACCTCACCATCAGAGCAAAA
  AATATTTTTTGGTTCCCA
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239693
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MTLQELVHKAASCYMDRVAVCFDECNNQLPVYYTYKTVVNAASELSNFLLLHCDFQGIREIGLYCQPGI
  DLPSWILGILQVPAAYVPIEPDSPPSLSTHFMKKCNLKYILVEKKQINKFKSFHETLLNYDTFTVEHND
  LVLFRLHWKNTEVNLMLNDGKEKYEKEKIKSISSEHVNEEKAEEHMDLRLKHCLAYVLHTSGTTGIPKI
  VRVPHKCIVPNIQHFRVLFDITQEDVLFLASPLTFDPSVVEIFLALSSGASLLIVPTSVKLLPSKLASV
  LFSHHRVTVLQATPTLLRRFGSQLIKSTVLSATTSLRVLALGGEAFPSLTVLRSWRGEGNKTQIFNVYG
  ITEVSSWATIYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTNGFTIQEGSGQVFLGGRNRVCFLDD
  EVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEELQQVESCAVTWYNQEKLI
  LFMVSKDASVKEYIFKELQKYLPSHAVPDELVLIDSLPFTSHGKIDVSELNKIYLNYINLKSENKLSGK
  EDLWEKLQYLWKSTLNLPEDLLRVPDESLFLNSGGDSLKSIRLLSEIEKLVGTSVPGLLEIILSSSILE
  IYNHILQTVVPDEDVTFRKSCATKRKLSDINQEEASGTSLHQKAIMTFTCHNEINAFVVLSRGSQILSL
  NSTRFLTKLGHCSSACPSDSVSQTNIQNLKGLNSPVLIGKSKDPSCVAKVSEEGKPAIGTQKMELHVRW
  RSDTGKCVDASPLVVIPTFDKSSTTVYIGSHSHRMKAVDFYSGKVKWEQILGDRIESSACVSKCGNFIV
  VGLAVLYQWTNLFIPVYLTIRAKNIFWFP
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286671
• ORF Size: 2571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001286671.1, NP_001273600.1
• RefSeq Size: 3195 bp
• RefSeq ORF: 2574 bp
• Locus ID: 132949
• Cytogenetics: 4q12
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways
• MW: 96.7 kDa
• Gene Summary: This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016]