Dynamin 1 (DNM1) (NM_001288739) Human Tagged ORF Clone

CAT#: RG239706

DNM1 (GFP-tagged) - Human dynamin 1 (DNM1), transcript variant 5

Specifications

Product Data

• Tag: TurboGFP
• Symbol: DNM1
• Synonyms: DNM; EIEE31
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239706 representing NM_001288739.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCAACCGCGGCATGGAAGATCTCATCCCGCTGGTCAACCGGCTGCAAGACGCCTTCTCTGCCATC
  GGCCAGAACGCGGACCTCGACCTGCCGCAGATCGCTGTGGTGGGCGGCCAGAGCGCCGGCAAGAGCTCG
  GTGCTCGAGAATTTCGTAGGCAGGGACTTCTTGCCTCGAGGATCTGGCATTGTCACCCGACGTCCCCTG
  GTCTTGCAGCTGGTCAATGCAACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGAAAGAAATTCACC
  GACTTCGAGGAGGTGCGCCTTGAGATCGAGGCCGAGACCGACAGGGTCACCGGCACCAACAAGGGCATC
  TCGCCGGTGCCTATCAACCTCCGCGTCTACTCGCCGCACGTGCTGAACCTGACCCTGGTGGACCTGCCC
  GGAATGACCAAGGTCCCGGTGGGGGACCAACCTCCCGACATCGAGTTCCAGATCCGAGACATGCTTATG
  CAGTTTGTCACCAAGGAGAACTGCCTCATCCTGGCCGTGTCCCCCGCCAACTCTGACCTGGCCAATTCT
  GACGCCCTCAAGGTCGCCAAGGAGGTGGACCCCCAGGGCCAGCGCACCATCGGGGTCATCACCAAGCTG
  GACCTGATGGACGAGGGCACAGATGCCCGTGATGTGCTGGAGAACAAGCTGCTCCCCCTGCGCAGAGGC
  TACATTGGAGTGGTGAACCGGAGCCAGAAGGACATTGATGGCAAGAAGGACATTACCGCCGCCTTGGCT
  GCTGAACGAAAGTTCTTCCTCTCCCATCCATCTTATCGCCACTTGGCTGACCGTATGGGCACGCCCTAC
  CTGCAGAAGGTCCTCAATCAGCAACTGACGAACCACATCCGGGACACACTGCCGGGGCTGCGGAACAAG
  CTGCAGAGCCAGCTACTGTCCATTGAGAAGGAGGTGGAGGAATACAAGAACTTCCGCCCTGATGACCCA
  GCTCGCAAGACCAAGGCCCTGCTGCAGATGGTCCAGCAGTTCGCCGTAGACTTTGAGAAGCGCATTGAG
  GGCTCAGGAGATCAGATCGACACCTACGAACTGTCAGGGGGAGCCCGCATTAACCGAATCTTCCACGAG
  CGCTTCCCTTTCGAGCTGGTCAAGATGGAGTTTGATGAGAAGGAACTCCGAAGGGAGATCAGCTATGCT
  ATCAAGAATATCCATGGCATTAGGACGGGCCTCTTCACACCTGACCTCGCTTTTGAAGCCACAGTGAAA
  AAGCAGGTGCAGAAGCTCAAAGAGCCCAGTATCAAGTGTGTGGATATGGTAGTCAGTGAGCTCACAGCC
  ACCATCAGAAAGTGTAGCGAAAAGCTCCAGCAGTACCCGCGGCTACGGGAGGAGATGGAGCGCATCGTG
  ACCACCCACATCCGGGAGCGCGAGGGCCGCACTAAGGAGCAGGTCATGCTTCTCATCGATATCGAGCTG
  GCTTACATGAACACCAACCATGAGGACTTCATAGGCTTTGCCAATGCTCAGCAGAGGAGCAACCAGATG
  AACAAGAAGAAGACTTCAGGGAACCAGGATGAGATTCTGGTCATCCGCAAGGGCTGGCTGACTATCAAT
  AATATTGGCATCATGAAAGGGGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGG
  TACAAGGATGATGAGGAGAAAGAGAAGAAATACATGCTGTCTGTGGACAACCTCAAGCTGCGGGACGTG
  GAGAAGGGCTTTATGTCGAGCAAGCATATCTTTGCCCTCTTTAACACGGAGCAGAGGAATGTCTACAAG
  GATTATCGGCAGCTGGAGCTAGCCTGTGAGACACAGGAGGAGGTGGACAGCTGGAAGGCCTCCTTCCTG
  AGGGCTGGCGTGTACCCTGAGCGTGTTGGGGACAAAGAGAAAGCCAGCGAGACCGAGGAGAATGGCTCC
  GACAGCTTCATGCATTCCATGGACCCACAGCTGGAACGGCAAGTGGAGACCATCCGGAATCTTGTGGAC
  TCATACATGGCCATTGTCAACAAGACCGTGAGGGACCTCATGCCCAAGACCATCATGCACCTCATGATT
  AACAATACCAAGGAGTTCATCTTCTCGGAGCTGCTGGCCAACCTGTACTCGTGTGGGGACCAGAACACG
  CTGATGGAGGAGTCGGCGGAGCAGGCACAGCGGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAG
  GAGGCGCTCAGCATCATCGGCGACATCAACACGACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGAC
  GACTCCTGGCTGCAGGTGCAGAGCGTACCGGCCGGACGCAGGTCGCCCACGTCCAGCCCCACGCCGCAG
  CGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCGGGTCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCT
  GGGTCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCTTTCGGCCCT
  CCCCCTCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCCGGGGTCCCCAGCCGATCGGGTCAGGCAAGT
  CCATCCCGTCCTGAGAGCCCCAGGCCCCCCTTCGACCTC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239706
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPL
  VLQLVNATTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLP
  GMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKVAKEVDPQGQRTIGVITKL
  DLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPY
  LQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVEEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIE
  GSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDLAFEATVK
  KQVQKLKEPSIKCVDMVVSELTATIRKCSEKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIEL
  AYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSW
  YKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFL
  RAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMI
  NNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVD
  DSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGP
  PPQVPSRPNRAPPGVPSRSGQASPSRPESPRPPFDL
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288739
• ORF Size: 2592 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001288739.2
• RefSeq Size: 3260 bp
• RefSeq ORF: 2595 bp
• Locus ID: 1759
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• MW: 97.7 kDa
• Gene Summary: 'This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'