TAF4B (NM_001293725) Human Tagged ORF Clone

CAT#: RG239710

TAF4B (GFP-tagged) - Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B), transcript variant 1

Specifications

Product Data

• Tag: TurboGFP
• Symbol: TAF4B
• Synonyms: SPGF13; TAF2C2; TAFII105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239710 representing NM_001293725.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTG
  ACCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACT
  AAGGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTG
  GTGACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAG
  ATAGTCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGA
  ACCGTTTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCC
  GAGACCACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACA
  GTGCCGAACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATA
  GGAACTACTGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTC
  GTCACAGTTACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCA
  TCCACTCCTTCAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCG
  ACAATGCTAGAAAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGA
  TCACAGTCCCCAGAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAA
  GCAGAAGAATTTACTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTT
  CTTAAGAAAAGCGTGGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTT
  CAGCAGACTTCTAGTGACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACA
  ACTACAGTGTCCTCAAGCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTG
  TCAGTGCAAACTTTGAACCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCT
  GTGGGCCCAACTGCTGCAACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTT
  GTGACATCTGTGGCAAACACAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACA
  GCAGTAACACTGTCCCTTCCAGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCT
  GTGAAACCTGTTGTTTCTTCTGCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATC
  AAACTTGCCCAGCCGGGCCCTGTCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAA
  CTATTCTCATTGTTTCAGGTAGTTCAGCAGCCTTCAGGAGGCAATGAAAAACAAGTGACCACAATTTCA
  CATTCCTCAACATTGACCATTCAGAAATGTGGACAGAAGACGATGCCAGTGAACACCATAATACCTACT
  AGTCAGTTTCCTCCAGCTTCCATTCTAAAGCAAATTACTCTGCCTGGAAATAAAATTCTGTCACTTCAA
  GCATCTCCTACTCAGAAAAATAGAATAAAAGAGAATGTAACATCATGCTTCCGAGATGAGGATGACATC
  AATGATGTGACTTCTATGGCAGGGGTCAACCTTAATGAAGAAAATGCCTGCATCTTAGCAACAAACTCT
  GAATTGGTTGGCACACTCATTCAGTCATGTAAAGATGAACCATTTCTTTTTATTGGAGCTCTACAAAAG
  AGAATTTTAGACATTGGTAAAAAGCATGACATTACAGAACTTAACTCTGATGCTGTGAACTTGATCTCC
  CAAGCAACACAGGAACGACTACGAGGCCTTCTAGAAAAACTGACTGCAATTGCTCAGCATCGAATGACT
  ACTTACAAGGCAAGTGAAAATTACATCCTGTGTAGTGATACCAGGTCACAGCTCAAATTTCTTGAAAAG
  CTGGATCAATTGGAGAAGCAGAGAAAGGATTTGGAAGAAAGAGAAATGTTACTTAAGGCAGCCAAGAGT
  CGTTCTAATAAAGAAGATCCAGAACAGCTGAGATTAAAGCAGAAAGCCAAAGAGTTACAGCAATTGGAA
  CTTGCACAGATACAGCATAGAGACGCTAATCTCACAGCTCTTGCAGCTATTGGACCAAGGAAGAAGAGA
  CCACTAGAATCTGGAATTGAGGGCTTAAAAGACAACCTTCTTGCTTCTGGGACATCCAGCCTGACAGCC
  ACCAAACAGTTGCATCGTCCAAGAATCACGAGAATCTGCCTCAGGGACTTGATATTTTGTATGGAACAG
  GAACGGGAGATGAAGTATTCTCGAGCTCTATACCTGGCCCTTCTGAAG
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239710
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPAGLTEPAGAAPPAAVSASGTVTMAPAGALPVRVESTPVALGAVTKAPVSVCVEPTASQPLRSPVGTL
  VTKVAPVSAPPKVSSGPRLPAPQIVAVKAPNTTTIQFPANLQLPPGTVLIKSNSGPLMLVSPQQTVTRA
  ETTSNITSRPAVPANPQTVKICTVPNSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSV
  VTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSG
  SQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCV
  QQTSSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHS
  VGPTAATGGTTAGTGLLQTSKPLVTSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPS
  VKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVKQLFSLFQVVQQPSGGNEKQVTTIS
  HSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDI
  NDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLIS
  QATQERLRGLLEKLTAIAQHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKS
  RSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRKKRPLESGIEGLKDNLLASGTSSLTA
  TKQLHRPRITRICLRDLIFCMEQEREMKYSRALYLALLK
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293725
• ORF Size: 2601 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001293725.2
• RefSeq Size: 4354 bp
• RefSeq ORF: 2604 bp
• Locus ID: 6875
• Cytogenetics: 18q11.2
• Protein Pathways: Basal transcription factors, Huntington's disease
• MW: 92.2 kDa
• Gene Summary: 'TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]'