TAF4B (NM_001293725) Human Tagged ORF Clone
CAT#: RG239710
- TrueORF®
TAF4B (GFP-tagged) - Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B), transcript variant 1
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Specifications
Product Data | |
Tag | TurboGFP |
Symbol | TAF4B |
Synonyms | SPGF13; TAF2C2; TAFII105 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG239710 representing NM_001293725.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTG ACCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACT AAGGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTG GTGACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAG ATAGTCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGA ACCGTTTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCC GAGACCACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACA GTGCCGAACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATA GGAACTACTGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTC GTCACAGTTACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCA TCCACTCCTTCAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCG ACAATGCTAGAAAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGA TCACAGTCCCCAGAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAA GCAGAAGAATTTACTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTT CTTAAGAAAAGCGTGGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTT CAGCAGACTTCTAGTGACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACA ACTACAGTGTCCTCAAGCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTG TCAGTGCAAACTTTGAACCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCT GTGGGCCCAACTGCTGCAACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTT GTGACATCTGTGGCAAACACAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACA GCAGTAACACTGTCCCTTCCAGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCT GTGAAACCTGTTGTTTCTTCTGCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATC AAACTTGCCCAGCCGGGCCCTGTCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAA CTATTCTCATTGTTTCAGGTAGTTCAGCAGCCTTCAGGAGGCAATGAAAAACAAGTGACCACAATTTCA CATTCCTCAACATTGACCATTCAGAAATGTGGACAGAAGACGATGCCAGTGAACACCATAATACCTACT AGTCAGTTTCCTCCAGCTTCCATTCTAAAGCAAATTACTCTGCCTGGAAATAAAATTCTGTCACTTCAA GCATCTCCTACTCAGAAAAATAGAATAAAAGAGAATGTAACATCATGCTTCCGAGATGAGGATGACATC AATGATGTGACTTCTATGGCAGGGGTCAACCTTAATGAAGAAAATGCCTGCATCTTAGCAACAAACTCT GAATTGGTTGGCACACTCATTCAGTCATGTAAAGATGAACCATTTCTTTTTATTGGAGCTCTACAAAAG AGAATTTTAGACATTGGTAAAAAGCATGACATTACAGAACTTAACTCTGATGCTGTGAACTTGATCTCC CAAGCAACACAGGAACGACTACGAGGCCTTCTAGAAAAACTGACTGCAATTGCTCAGCATCGAATGACT ACTTACAAGGCAAGTGAAAATTACATCCTGTGTAGTGATACCAGGTCACAGCTCAAATTTCTTGAAAAG CTGGATCAATTGGAGAAGCAGAGAAAGGATTTGGAAGAAAGAGAAATGTTACTTAAGGCAGCCAAGAGT CGTTCTAATAAAGAAGATCCAGAACAGCTGAGATTAAAGCAGAAAGCCAAAGAGTTACAGCAATTGGAA CTTGCACAGATACAGCATAGAGACGCTAATCTCACAGCTCTTGCAGCTATTGGACCAAGGAAGAAGAGA CCACTAGAATCTGGAATTGAGGGCTTAAAAGACAACCTTCTTGCTTCTGGGACATCCAGCCTGACAGCC ACCAAACAGTTGCATCGTCCAAGAATCACGAGAATCTGCCTCAGGGACTTGATATTTTGTATGGAACAG GAACGGGAGATGAAGTATTCTCGAGCTCTATACCTGGCCCTTCTGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG239710
Blue=ORF Red=Cloning site Green=Tag(s) MPAGLTEPAGAAPPAAVSASGTVTMAPAGALPVRVESTPVALGAVTKAPVSVCVEPTASQPLRSPVGTL VTKVAPVSAPPKVSSGPRLPAPQIVAVKAPNTTTIQFPANLQLPPGTVLIKSNSGPLMLVSPQQTVTRA ETTSNITSRPAVPANPQTVKICTVPNSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSV VTVTPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSG SQSPEMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCV QQTSSDMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHS VGPTAATGGTTAGTGLLQTSKPLVTSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPS VKPVVSSAGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVKQLFSLFQVVQQPSGGNEKQVTTIS HSSTLTIQKCGQKTMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDI NDVTSMAGVNLNEENACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLIS QATQERLRGLLEKLTAIAQHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKS RSNKEDPEQLRLKQKAKELQQLELAQIQHRDANLTALAAIGPRKKRPLESGIEGLKDNLLASGTSSLTA TKQLHRPRITRICLRDLIFCMEQEREMKYSRALYLALLK TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001293725 |
ORF Size | 2601 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reference Data | |
RefSeq | NM_001293725.2 |
RefSeq Size | 4354 bp |
RefSeq ORF | 2604 bp |
Locus ID | 6875 |
Cytogenetics | 18q11.2 |
Protein Pathways | Basal transcription factors, Huntington's disease |
MW | 92.2 kDa |
Gene Summary | 'TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]' |
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