SMARCA6 (HELLS) (NM_001289067) Human Tagged ORF Clone

CAT#: RG239733

HELLS (GFP-tagged) - Human helicase, lymphoid-specific (HELLS), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: HELLS
• Synonyms: ICF4; LSH; Nbla10143; PASG; SMARCA6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239733 representing NM_001289067.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCAGCGGAACGGCCCGCGGGCAGCGGCGGCTCGGAGGCTCCAGCAATGGTTGAACAACTGGACACT
  GCTGTGATTACCCCGGCCATGCTAGAAGAGGAAGAACAGCTTGAAGCTGCTGGACTAGAGAGAGAGCGG
  AAGATGCTGGAAAAGGCTCGCATGTCTTGGGATAGAGAGTCGACAGAAATTCGGTACCGTAGACTTCAA
  CATTTGCTTGAAAAAAGCAATATATACTCCAAATTTTTATTGACGAAAATGGAACAGCAACAATTAGAG
  GAACAGAAGAAGAAAGAAAAATTGGAGAGAAAAAAGGAGTCTTTAAAAGTTAAAAAGGGTAAAAATTCA
  ATTGATGCAAGTGAAGAGAAGCCAGTTATGAGGAAAAAAAGAGGAAGAGAAGATGAATCATACAATATT
  TCAGAGGTCATGTCAAAAGAGGAAATTTTGTCTGTGGCTAAAAAAAATAAAAAGGAGAATGAGGATGAA
  AACTCCTCCTCTACTAATCTCTGTGTGGAAGATCTTCAGAAAAATAAAGATTCGAATAGTATAATTAAA
  GATAGATTGTCTGAAACGGTTAGGCAGAATACTAAATTCTTTTTTGACCCAGTCCGGAAGTGTAATGGT
  CAGCCAGTACCTTTTCAACAACCAAAGCACTTCACTGGAGGAGTGATGCGATGGTACCAAGTAGAAGGC
  ATGGAATGGCTTAGGATGCTTTGGGAAAATGGAATTAATGGCATTTTAGCAGATGAAATGGGATTGGGT
  AAGACAGTTCAGTGCATTGCTACTATTGCATTGATGATTCAGAGAGGAGTACCAGGACCTTTTCTTGTC
  TGTGGCCCTTTGTCTACACTTCCTAACTGGATGGCTGAATTCAAAAGATTTACACCAGATATCCCTACA
  ATGTTATATCATGGAACCCAGGAGGAACGTCAAAAATTGGTAAGAAATATTTACAAACGGAAAGGGACT
  TTGCAGATTCATCCTGTGGTAATCACGTCATTTGAAATAGCCATGAGAGACCGAAATGCGTTACAGCAT
  TGCTATTGGAAATACTTAATAGTAGATGAAGGACACAGGATTAAGAATATGAAGTGCCGTCTAATCAGG
  GAGTTAAAACGATTCAATGCTGATAACAAACTTCTTTTGACTGGTACTCCCTTGCAAAACAATTTATCA
  GAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGACTTGAAAAGCTTTGAGTCTTGG
  TTTGACATCACTAGTCTTTCTGAAACTGCTGAAGATATTATTGCTAAAGAAAGAGAACAGAATGTATTG
  CATATGCTGCACCAGGAAAGAATTCCTAGAATGTATGTGTCAGAGTTTACAGATGAGGGCTTCTTTGAA
  GGCAAATTGCCCTCCAGAAAAGATTTATATGATATACGTCCATATGAAAATACTTATTTACTCAAGCTT
  CATACCAATTTTGAGATTTTAACACCTTTCTTATTGAGAAGACTGAAGTCTGATGTTGCTCTTGAAGTT
  CCTCCTAAACGAGAAGTAGTCGTTTATGCTCCACTTTCAAAGAAGCAGGAGATCTTTTATACAGCCATT
  GTGAACCGTACAATTGCAAACATGTTTGGATCCAGTGAGAAAGAAACAATTGAGTTAAGTCCTACTGGT
  CGACCAAAACGACGAACTAGAAAATCAATAAATTACAGCAAAATAGATGATTTCCCTAATGAATTGGAA
  AAACTGATCAGTCAAATACAGCCAGAGGTGGACCGAGAAAGAGCTGTTGTGGAAGTGAATATCCCTGTA
  GAATCTGAAGTTAATCTGAAGCTGCAGAATATAATGATGCTACTTCGTAAATGTTGTAATCATCCATAT
  TTGATTGAATATCCTATAGACCCTGTTACACAAGAATTTAAGATCGATGAAGAATTGGTAACAAATTCT
  GGGAAGTTCTTGATTTTGGATCGAATGCTGCCAGAACTAAAAAAAAGAGGTCACAAGGTGCTGCTTTTT
  TCACAAATGACAAGCATGTTGGACATTTTGATGGATTACTGCCATCTCAGAGATTTCAACTTCAGCAGG
  CTTGATGGGTCCATGTCTTACTCAGAGAGAGAAAAAAACATGCACAGCTTCAACACGGATCCAGAGGTG
  TTTATCTTCTTAGTGAGTACACGAGCTGGTGGCCTGGGCATTAATCTGACTGCAGCAGATACAGTTATC
  ATTTATGATAGTGATTGGAACCCCCAGTCGGATCTTCAGGCCCAGGATAGATGTCATAGAATTGGTCAG
  ACAAAGCCAGTTGTTGTTTATCGCCTTGTTACAGCAAATACTATCGATCAGAAAATTGTGGAAAGAGCA
  GCTGCTAAAAGGAAACTGGAAAAGTTGATCATCCATAAAAATCATTTCAAAGGTGGTCAGTCTGGATTA
  AATCTGTCTAAGAATTTCTTAGATCCTAAGGAATTAATGGAATTATTAAAATCTAGAGATTATGAAAGG
  GAAATAAAAGGATCAAGAGAGAAGGTCATTAGTGATAAAGATCTAGAGTTGTTGTTAGATCGAAGTGAT
  CTTATTGATCAAATGAATGCTTCAGGACCAATTAAAGAGAAGATGGGGATATTCAAGATATTAGAAAAT
  TCTGAAGATTCCAGTCCTGAATGTTTGTTT
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239733
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MPAERPAGSGGSEAPAMVEQLDTAVITPAMLEEEEQLEAAGLERERKMLEKARMSWDRESTEIRYRRLQ
  HLLEKSNIYSKFLLTKMEQQQLEEQKKKEKLERKKESLKVKKGKNSIDASEEKPVMRKKRGREDESYNI
  SEVMSKEEILSVAKKNKKENEDENSSSTNLCVEDLQKNKDSNSIIKDRLSETVRQNTKFFFDPVRKCNG
  QPVPFQQPKHFTGGVMRWYQVEGMEWLRMLWENGINGILADEMGLGKTVQCIATIALMIQRGVPGPFLV
  CGPLSTLPNWMAEFKRFTPDIPTMLYHGTQEERQKLVRNIYKRKGTLQIHPVVITSFEIAMRDRNALQH
  CYWKYLIVDEGHRIKNMKCRLIRELKRFNADNKLLLTGTPLQNNLSELWSLLNFLLPDVFDDLKSFESW
  FDITSLSETAEDIIAKEREQNVLHMLHQERIPRMYVSEFTDEGFFEGKLPSRKDLYDIRPYENTYLLKL
  HTNFEILTPFLLRRLKSDVALEVPPKREVVVYAPLSKKQEIFYTAIVNRTIANMFGSSEKETIELSPTG
  RPKRRTRKSINYSKIDDFPNELEKLISQIQPEVDRERAVVEVNIPVESEVNLKLQNIMMLLRKCCNHPY
  LIEYPIDPVTQEFKIDEELVTNSGKFLILDRMLPELKKRGHKVLLFSQMTSMLDILMDYCHLRDFNFSR
  LDGSMSYSEREKNMHSFNTDPEVFIFLVSTRAGGLGINLTAADTVIIYDSDWNPQSDLQAQDRCHRIGQ
  TKPVVVYRLVTANTIDQKIVERAAAKRKLEKLIIHKNHFKGGQSGLNLSKNFLDPKELMELLKSRDYER
  EIKGSREKVISDKDLELLLDRSDLIDQMNASGPIKEKMGIFKILENSEDSSPECLF
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289067
• ORF Size: 2652 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001289067.1, NP_001275996.1
• RefSeq Size: 3301 bp
• RefSeq ORF: 2655 bp
• Locus ID: 3070
• Cytogenetics: 10q23.33
• MW: 103.2 kDa
• Gene Summary: 'This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014]'