Angiomotin like 1 (AMOTL1) (NM_001301007) Human Tagged ORF Clone

CAT#: RG239759

AMOTL1 (GFP-tagged) - Human angiomotin like 1 (AMOTL1), transcript variant 2

Specifications

Product Data

• Tag: TurboGFP
• Symbol: AMOTL1
• Synonyms: JEAP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG239759 representing NM_001301007.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGTTGAAGATCCTCTTTGTAAC
  TTCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGA
  ACAGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTG
  GCCATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAA
  AACCTCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACAC
  CAGGTGGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGAACTG
  CCCACTTACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAG
  GGGGCGGTGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCC
  ACTGTGAACCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCAC
  GTCCGCTCGCTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCC
  GGCTCGGGGAATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTG
  CTGGACCCTCGGGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAG
  ACCCAGGAGCACGGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTAC
  CCTGCTCCTCAGCCTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTA
  ACGAGCCGCCCATGCCAACTTCCGTTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACC
  TCTTCCGCCAGCGGGCCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCA
  CAGCCCCCGCCTGCCGCCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCC
  CAGCAAATGGTGGAGATATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGAC
  AATGCCGACAAGCTCCACAAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTGGTC
  AAGTCTACCACCAAGCGAGAATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGCGAGATTAGAAGA
  CTTCATGATTTCAACAGAGACCTCCGAGATCGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAA
  TACGAAGGGCATGAAGACAAAGCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAA
  AAGGAGAAATTAGAAATGGAGTTAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAG
  ATCCTGGACCAGGCTTTGAGCAACGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAG
  CAAGCATATGTTGAGAAAGTTGAGAAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAG
  CGAGAACAGATGGAGCGGAGACTGCGGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACCCAGCAG
  AAACATGGAAATGGCCAGCCAGCCAACATGCCGGAATACAATGCCCCAGCCCTCCTGGAACTTGTGCGG
  GAGAAGGAGGAGCGGATCCTGGCCCTGGAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAG
  AGCACCATCCGACACTTTGCCATGAATGCCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATC
  AACCACTCACGGAATGGCAGCTACGGAGAGAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAG
  GTGGTGCAGGCCAACAGAAGGTGTCAGGACATGGAATACACTATTAAAAATCTCCATGCCAAAATCATA
  GAGAAAGATGCTATGATTAAGGTCCTGCAGCAGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCC
  AGCCTACGTCCTGCCCGCTCCGTTCCATCCATAGCAGCAGCTACTGGGACACACTCTCGCCAGACCTCT
  CTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAGGAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTG
  CTGGGGAAGGAGCACCATGAGCATGCCTCTGCCCCACTGCTGCCACCCCCACCCACCTCAGCACTGTCC
  TCCATAGCCTCCACTACGGCAGCCAGCAGTGCCCACGCCAAGACAGGCAGCAAGGACAGCAGCACACAG
  ACTGACAAGAGTGCCGAGCTCTTCTGGCCCAGCATGGCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACG
  ACCCCTGCTCACAGCCCCGTCCTGAAACACCCAGCGGCCAAAGGGACCGCAGAGAAACTGGAGAACTCT
  CCTGGCCATGGGAAGTCGCCTGACCACAGAGGCCGGGTCAGCAGCTTGCTGCACAAGCCCGAGTTCCCT
  GATGGAGAGATGATGGAAGTCCTCATC
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
  
  Protein Sequence

  >Peptide sequence encoded by RG239759
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MWRAKLRRGTCEPAVKVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLL
  AIQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEEL
  PTYEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGH
  VRSLSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSK
  TQEHGLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQT
  SSASGPLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYD
  NADKLHKFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSRE
  YEGHEDKAAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREK
  QAYVEKVEKLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVR
  EKEERILALEADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEE
  VVQANRRCQDMEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTS
  LTSSQLAEEKKEEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQ
  TDKSAELFWPSMASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFP
  DGEMMEVLI
  TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
  MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
  SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
  FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301007
• ORF Size: 2718 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_001301007.2
• RefSeq Size: 8849 bp
• RefSeq ORF: 2721 bp
• Locus ID: 154810
• Cytogenetics: 11q21
• Protein Pathways: Tight junction
• MW: 101.6 kDa
• Gene Summary: The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]